(Linear) (Six-base) MAPSORT of: KIEE5566.seq  Check: 8893  from: 1  to: 1317



KIEE5566.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:05  ..

AccI GT'mk_AC

Cuts at:      0     876    1317 
   Size:        876     441
 
Acc65I G'GTAC_C

Cuts at:      0     224    1317 
   Size:        224    1093
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     482     721    1317 
   Size:        482     239     596

  Fragments arranged by size: 

                 596     482     239
 
AlwNI CAG_nnn'CTG

Cuts at:      0     341    1317 
   Size:        341     976
 
ApoI r'AATT_y

Cuts at:      0     933    1317 
   Size:        933     384
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     216     249    1317 
   Size:        216      33    1068

  Fragments arranged by size: 

                1068     216      33
 
BamHI G'GATC_C

Cuts at:      0     531    1317 
   Size:        531     786
 
BanI G'GyrC_C

Cuts at:      0     224    1317 
   Size:        224    1093
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     188    1317 
   Size:        188    1129
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      52      86    1232    1266    1317 
   Size:         52      34    1146      34      51

  Fragments arranged by size: 

                1146      52      51      34      34
 
BfrBI ATG'CAT

Cuts at:      0     909    1317 
   Size:        909     408
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286    1317 
   Size:        286    1031
 
BsaHI Gr'CG_yC

Cuts at:      0       4    1317 
   Size:          4    1313
 
BsaWI w'CCGG_w

Cuts at:      0      38    1317 
   Size:         38    1279
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      79    1317 
   Size:         79    1238
 
BseYI C'CCAG_C

Cuts at:      0     960    1294    1317 
   Size:        960     334      23

  Fragments arranged by size: 

                 960     334      23
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     365    1317 
   Size:         46     319     952

  Fragments arranged by size: 

                 952     319      46
 
BsiEI CG_ry'CG

Cuts at:      0      39      62    1317 
   Size:         39      23    1255

  Fragments arranged by size: 

                1255      39      23
 
BsmI GAATG_Cn'

Cuts at:      0     909    1317 
   Size:        909     408
 
BspHI T'CATG_A

Cuts at:      0    1121    1317 
   Size:       1121     196
 
BsrGI T'GTAC_A

Cuts at:      0     754    1317 
   Size:        754     563
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     377    1317 
   Size:        377     940
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     221    1317 
   Size:        221    1096
 
BstYI r'GATC_y

Cuts at:      0     240     531    1317 
   Size:        240     291     786

  Fragments arranged by size: 

                 786     291     240
 
BtsI GCAGTG_nn'

Cuts at:      0    1308    1317 
   Size:       1308       9
 
ClaI AT'CG_AT

Cuts at:      0     408    1317 
   Size:        408     909
 
DraI TTT'AAA

Cuts at:      0     309    1317 
   Size:        309    1008
 
EaeI y'GGCC_r

Cuts at:      0      59    1317 
   Size:         59    1258
 
EagI C'GGCC_G

Cuts at:      0      59    1317 
   Size:         59    1258
 
EarI CTCTTCn'nnn_

Cuts at:      0     521     661     740    1050    1317 
   Size:        521     140      79     310     267

  Fragments arranged by size: 

                 521     310     267     140      79
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     482     721    1317 
   Size:        482     239     596

  Fragments arranged by size: 

                 596     482     239
 
EcoO109I rG'GnC_Cy

Cuts at:      0     157     478    1317 
   Size:        157     321     839

  Fragments arranged by size: 

                 839     321     157
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     990    1022    1092    1124    1317 
   Size:        990      32      70      32     193

  Fragments arranged by size: 

                 990     193      70      32      32
 
HincII GTy'rAC

Cuts at:      0     750    1317 
   Size:        750     567
 
HpaI GTT'AAC

Cuts at:      0     750    1317 
   Size:        750     567
 
KpnI G_GTAC'C

Cuts at:      0     228    1317 
   Size:        228    1089
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     379    1317 
   Size:        379     938
 
MslI CAynn'nnrTG

Cuts at:      0     293    1317 
   Size:        293    1024
 
NsiI A_TGCA'T

Cuts at:      0     911    1317 
   Size:        911     406
 
NspI r_CATG'y

Cuts at:      0     189     202     690    1317 
   Size:        189      13     488     627

  Fragments arranged by size: 

                 627     488     189      13
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1085    1317 
   Size:       1085     232
 
PpuMI rG'GwC_Cy

Cuts at:      0     157     478    1317 
   Size:        157     321     839

  Fragments arranged by size: 

                 839     321     157
 
PsiI TTA'TAA

Cuts at:      0     575    1317 
   Size:        575     742
 
PstI C_TGCA'G

Cuts at:      0     154    1317 
   Size:        154    1163
 
SanDI GG'GwC_CC

Cuts at:      0     157    1317 
   Size:        157    1160
 
SfcI C'TryA_G

Cuts at:      0     150     785    1317 
   Size:        150     635     532

  Fragments arranged by size: 

                 635     532     150
 
SmlI C'TyrA_G

Cuts at:      0     265    1317 
   Size:        265    1052
 
SphI G_CATG'C

Cuts at:      0     690    1317 
   Size:        690     627
 
SspI AAT'ATT

Cuts at:      0     614    1317 
   Size:        614     703
 
TatI w'GTAC_w

Cuts at:      0     754    1317 
   Size:        754     563
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AlwNI     ApoI     BaeI    BamHI     BanI
     BbsI     BcgI    BfrBI    BpuEI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI     BsmI    BspHI    BsrGI   BstAPI    BstXI    BstYI
     BtsI     ClaI     DraI     EaeI     EagI     EarI  Eco57MI EcoO109I
    Hin4I   HincII     HpaI     KpnI     MmeI     MslI     NsiI     NspI
    PflMI    PpuMI     PsiI     PstI    SanDI     SfcI     SmlI     SphI
     SspI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BanII     BbeI    BbvCI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaXI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI    CspCI   DraIII
     DrdI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     KasI     MfeI     MluI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI