Restriction map for
pFN21AE5564
(Linear) (Six-base) MAPSORT of: KIEE5564.seq Check: 6409 from: 1 to: 1272
KIEE5564.orf
With 182 enzymes: SgfI *
January 29, 2014 12:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 607 1272
Size: 607 665
AatII G_ACGT'C
Cuts at: 0 719 1272
Size: 719 553
AccI GT'mk_AC
Cuts at: 0 350 1272
Size: 350 922
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1272
Size: 703 569
AfeI AGC'GCT
Cuts at: 0 54 383 1272
Size: 54 329 889
Fragments arranged by size:
889 329 54
AhdI GACnn_n'nnGTC
Cuts at: 0 1144 1272
Size: 1144 128
AlwNI CAG_nnn'CTG
Cuts at: 0 219 876 1025 1272
Size: 219 657 149 247
Fragments arranged by size:
657 247 219 149
AvaI C'yCGr_G
Cuts at: 0 11 343 436 662 720 1272
Size: 11 332 93 226 58 552
Fragments arranged by size:
552 332 226 93 58 11
BanI G'GyrC_C
Cuts at: 0 432 1002 1272
Size: 432 570 270
Fragments arranged by size:
570 432 270
BanII G_rGCy'C
Cuts at: 0 12 18 767 854 1272
Size: 12 6 749 87 418
Fragments arranged by size:
749 418 87 12 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 933 975 1176 1272
Size: 933 42 201 96
Fragments arranged by size:
933 201 96 42
BbvCI CC'TCA_GC
Cuts at: 0 589 1272
Size: 589 683
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 26 1272
Size: 26 1246
BclI T'GATC_A
Cuts at: 0 1058 1272
Size: 1058 214
BglI GCCn_nnn'nGGC
Cuts at: 0 467 556 1272
Size: 467 89 716
Fragments arranged by size:
716 467 89
BlpI GC'TnA_GC
Cuts at: 0 892 1272
Size: 892 380
Bme1580I G_kGCm'C
Cuts at: 0 547 1007 1272
Size: 547 460 265
Fragments arranged by size:
547 460 265
BmrI ACTGGGnnnn_n'
Cuts at: 0 169 1272
Size: 169 1103
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 511 604 1272
Size: 511 93 668
Fragments arranged by size:
668 511 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 832 841 1254 1272
Size: 312 520 9 413 18
Fragments arranged by size:
520 413 312 18 9
Bpu10I CC'TnA_GC
Cuts at: 0 589 1272
Size: 589 683
BsaI GGTCTCn'nnnn_
Cuts at: 0 528 1272
Size: 528 744
BsaAI yAC'GTr
Cuts at: 0 90 1194 1272
Size: 90 1104 78
Fragments arranged by size:
1104 90 78
BsaBI GATnn'nnATC
Cuts at: 0 415 1272
Size: 415 857
BsaHI Gr'CG_yC
Cuts at: 0 482 635 716 1175 1272
Size: 482 153 81 459 97
Fragments arranged by size:
482 459 153 97 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1109 1139 1272
Size: 1109 30 133
Fragments arranged by size:
1109 133 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 454 844 925 1272
Size: 454 390 81 347
Fragments arranged by size:
454 390 347 81
BseYI C'CCAG_C
Cuts at: 0 219 465 507 1095 1272
Size: 219 246 42 588 177
Fragments arranged by size:
588 246 219 177 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1011 1216 1272
Size: 1011 205 56
Fragments arranged by size:
1011 205 56
BsiEI CG_ry'CG
Cuts at: 0 323 1148 1272
Size: 323 825 124
Fragments arranged by size:
825 323 124
BsiHKAI G_wGCw'C
Cuts at: 0 12 1272
Size: 12 1260
BsiWI C'GTAC_G
Cuts at: 0 530 1272
Size: 530 742
Bsp1286I G_dGCh'C
Cuts at: 0 12 18 547 767 854 1007 1272
Size: 12 6 529 220 87 153 265
Fragments arranged by size:
529 265 220 153 87 12 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 121 197 230 317 362 607 1188 1272
Size: 121 76 33 87 45 245 581 84
Fragments arranged by size:
581 245 121 87 84 76 45 33
BsrBI CCG'CTC
Cuts at: 0 444 1272
Size: 444 828
BsrDI GCAATG_nn'
Cuts at: 0 173 1272
Size: 173 1099
BsrFI r'CCGG_y
Cuts at: 0 62 228 461 1272
Size: 62 166 233 811
Fragments arranged by size:
811 233 166 62
BsrGI T'GTAC_A
Cuts at: 0 1113 1272
Size: 1113 159
BstAPI GCAn_nnn'nTGC
Cuts at: 0 396 1272
Size: 396 876
BstEII G'GTnAC_C
Cuts at: 0 864 1041 1272
Size: 864 177 231
Fragments arranged by size:
864 231 177
BstXI CCAn_nnnn'nTGG
Cuts at: 0 576 936 1272
Size: 576 360 336
Fragments arranged by size:
576 360 336
BstYI r'GATC_y
Cuts at: 0 725 1272
Size: 725 547
BtgI C'CryG_G
Cuts at: 0 449 794 962 1142 1272
Size: 449 345 168 180 130
Fragments arranged by size:
449 345 180 168 130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 675 1272
Size: 675 597
BtsI GCAGTG_nn'
Cuts at: 0 646 1120 1272
Size: 646 474 152
Fragments arranged by size:
646 474 152
EaeI y'GGCC_r
Cuts at: 0 95 320 548 566 959 1188 1272
Size: 95 225 228 18 393 229 84
Fragments arranged by size:
393 229 228 225 95 84 18
EagI C'GGCC_G
Cuts at: 0 320 1272
Size: 320 952
EarI CTCTTCn'nnn_
Cuts at: 0 124 364 772 1272
Size: 124 240 408 500
Fragments arranged by size:
500 408 240 124
EcoICRI GAG'CTC
Cuts at: 0 10 1272
Size: 10 1262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 703 832 841 1254 1272
Size: 312 391 129 9 413 18
Fragments arranged by size:
413 391 312 129 18 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 736 1272
Size: 736 536
EcoO109I rG'GnC_Cy
Cuts at: 0 515 873 1138 1272
Size: 515 358 265 134
Fragments arranged by size:
515 358 265 134
HaeII r_GCGC'y
Cuts at: 0 56 274 304 385 1272
Size: 56 218 30 81 887
Fragments arranged by size:
887 218 81 56 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 39 71 1130 1162 1272
Size: 39 32 1059 32 110
Fragments arranged by size:
1059 110 39 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 465 773 1272
Size: 465 308 499
Fragments arranged by size:
499 465 308
MscI TGG'CCA
Cuts at: 0 568 961 1272
Size: 568 393 311
Fragments arranged by size:
568 393 311
MslI CAynn'nnrTG
Cuts at: 0 775 793 949 1272
Size: 775 18 156 323
Fragments arranged by size:
775 323 156 18
MspA1I CmG'CkG
Cuts at: 0 216 451 810 891 1272
Size: 216 235 359 81 381
Fragments arranged by size:
381 359 235 216 81
NaeI GCC'GGC
Cuts at: 0 463 1272
Size: 463 809
NcoI C'CATG_G
Cuts at: 0 794 962 1272
Size: 794 168 310
Fragments arranged by size:
794 310 168
NgoMIV G'CCGG_C
Cuts at: 0 461 1272
Size: 461 811
NotI GC'GGCC_GC
Cuts at: 0 320 1272
Size: 320 952
NspI r_CATG'y
Cuts at: 0 1056 1272
Size: 1056 216
PasI CC'CwG_GG
Cuts at: 0 20 456 870 1272
Size: 20 436 414 402
Fragments arranged by size:
436 414 402 20
PflMI CCAn_nnn'nTGG
Cuts at: 0 876 968 1272
Size: 876 92 304
Fragments arranged by size:
876 304 92
PmlI CAC'GTG
Cuts at: 0 1194 1272
Size: 1194 78
PpuMI rG'GwC_Cy
Cuts at: 0 515 1138 1272
Size: 515 623 134
Fragments arranged by size:
623 515 134
PshAI GACnn'nnGTC
Cuts at: 0 520 1272
Size: 520 752
PstI C_TGCA'G
Cuts at: 0 215 1272
Size: 215 1057
PvuII CAG'CTG
Cuts at: 0 216 810 891 1272
Size: 216 594 81 381
Fragments arranged by size:
594 381 216 81
SacI G_AGCT'C
Cuts at: 0 12 1272
Size: 12 1260
SacII CC_GC'GG
Cuts at: 0 452 1272
Size: 452 820
SanDI GG'GwC_CC
Cuts at: 0 515 1138 1272
Size: 515 623 134
Fragments arranged by size:
623 515 134
SapI GCTCTTCn'nnn_
Cuts at: 0 364 772 1272
Size: 364 408 500
Fragments arranged by size:
500 408 364
SfcI C'TryA_G
Cuts at: 0 211 1272
Size: 211 1061
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 467 1272
Size: 467 805
SmaI CCC'GGG
Cuts at: 0 345 664 1272
Size: 345 319 608
Fragments arranged by size:
608 345 319
SphI G_CATG'C
Cuts at: 0 1056 1272
Size: 1056 216
StyI C'CwwG_G
Cuts at: 0 296 794 962 1272
Size: 296 498 168 310
Fragments arranged by size:
498 310 296 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 60 1272
Size: 60 1212
TatI w'GTAC_w
Cuts at: 0 1113 1272
Size: 1113 159
Tth111I GACn'n_nGTC
Cuts at: 0 1264 1272
Size: 1264 8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 792 1272
Size: 792 480
XmaI C'CCGG_G
Cuts at: 0 343 662 1272
Size: 343 319 610
Fragments arranged by size:
610 343 319
ZraI GAC'GTC
Cuts at: 0 717 1272
Size: 717 555
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AhdI AlwNI AvaI
BanI BanII BbsI BbvCI BcgI BclI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NotI NspI PasI
PflMI PmlI PpuMI PshAI PstI PvuII SacI SacII
SanDI SapI SfcI SfiI SmaI SphI StyI TaqII
TatI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AleI AlfI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BfrBI BglII BmgBI BmtI BpuEI
BsaWI BsmI BsmBI BspEI BspHI BssHII BssSI BstBI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NruI NsiI PacI PciI PfoI PmeI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SalI SbfI ScaI
SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI StuI SwaI TstI XbaI XhoI XmnI