Restriction map for
pFN21AE5560
(Linear) (Six-base) MAPSORT of: KIEE5560.seq Check: 7279 from: 1 to: 1134
KIEE5560.orf
With 182 enzymes: SgfI *
January 29, 2014 12:05 ..
AccI GT'mk_AC
Cuts at: 0 156 1134
Size: 156 978
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 965 1134
Size: 530 435 169
Fragments arranged by size:
530 435 169
AhdI GACnn_n'nnGTC
Cuts at: 0 429 1134
Size: 429 705
AlwNI CAG_nnn'CTG
Cuts at: 0 565 622 849 870 943 987 1134
Size: 565 57 227 21 73 44 147
Fragments arranged by size:
565 227 147 73 57 44 21
ApoI r'AATT_y
Cuts at: 0 568 1009 1134
Size: 568 441 125
Fragments arranged by size:
568 441 125
BanII G_rGCy'C
Cuts at: 0 79 252 1134
Size: 79 173 882
Fragments arranged by size:
882 173 79
BbsI GAAGACnn'nnnn_
Cuts at: 0 419 1134
Size: 419 715
BbvCI CC'TCA_GC
Cuts at: 0 10 648 1134
Size: 10 638 486
Fragments arranged by size:
638 486 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 515 820 1134
Size: 321 194 305 314
Fragments arranged by size:
321 314 305 194
Bpu10I CC'TnA_GC
Cuts at: 0 10 648 1134
Size: 10 638 486
Fragments arranged by size:
638 486 10
BsaWI w'CCGG_w
Cuts at: 0 435 1134
Size: 435 699
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 513 543 1134
Size: 513 30 591
Fragments arranged by size:
591 513 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 572 1134
Size: 572 562
BseYI C'CCAG_C
Cuts at: 0 78 663 1134
Size: 78 585 471
Fragments arranged by size:
585 471 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 899 1134
Size: 899 235
BsiHKAI G_wGCw'C
Cuts at: 0 252 1134
Size: 252 882
BsmI GAATG_Cn'
Cuts at: 0 85 971 1134
Size: 85 886 163
Fragments arranged by size:
886 163 85
Bsp1286I G_dGCh'C
Cuts at: 0 79 252 1134
Size: 79 173 882
Fragments arranged by size:
882 173 79
BspEI T'CCGG_A
Cuts at: 0 435 1134
Size: 435 699
BstAPI GCAn_nnn'nTGC
Cuts at: 0 722 1134
Size: 722 412
BstYI r'GATC_y
Cuts at: 0 729 866 1134
Size: 729 137 268
Fragments arranged by size:
729 268 137
BtgI C'CryG_G
Cuts at: 0 607 739 1134
Size: 607 132 395
Fragments arranged by size:
607 395 132
BtsI GCAGTG_nn'
Cuts at: 0 426 1134
Size: 426 708
DraIII CAC_nnn'GTG
Cuts at: 0 939 957 1134
Size: 939 18 177
Fragments arranged by size:
939 177 18
EarI CTCTTCn'nnn_
Cuts at: 0 67 1134
Size: 67 1067
EcoICRI GAG'CTC
Cuts at: 0 250 1134
Size: 250 884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 515 530 820 965 1134
Size: 321 194 15 290 145 169
Fragments arranged by size:
321 290 194 169 145 15
EcoRI G'AATT_C
Cuts at: 0 568 1134
Size: 568 566
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 138 170 415 447 858 890 1134
Size: 138 32 245 32 411 32 244
Fragments arranged by size:
411 245 244 138 32 32 32
HincII GTy'rAC
Cuts at: 0 157 1134
Size: 157 977
HindIII A'AGCT_T
Cuts at: 0 105 926 1134
Size: 105 821 208
Fragments arranged by size:
821 208 105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 1134
Size: 610 524
MspA1I CmG'CkG
Cuts at: 0 9 82 849 1134
Size: 9 73 767 285
Fragments arranged by size:
767 285 73 9
PstI C_TGCA'G
Cuts at: 0 224 594 618 866 1134
Size: 224 370 24 248 268
Fragments arranged by size:
370 268 248 224 24
PvuII CAG'CTG
Cuts at: 0 9 849 1134
Size: 9 840 285
Fragments arranged by size:
840 285 9
SacI G_AGCT'C
Cuts at: 0 252 1134
Size: 252 882
SalI G'TCGA_C
Cuts at: 0 155 1134
Size: 155 979
SapI GCTCTTCn'nnn_
Cuts at: 0 67 1134
Size: 67 1067
SbfI CC_TGCA'GG
Cuts at: 0 224 1134
Size: 224 910
ScaI AGT'ACT
Cuts at: 0 1083 1134
Size: 1083 51
SexAI A'CCwGG_T
Cuts at: 0 291 1134
Size: 291 843
SfcI C'TryA_G
Cuts at: 0 220 590 614 862 1134
Size: 220 370 24 248 272
Fragments arranged by size:
370 272 248 220 24
SpeI A'CTAG_T
Cuts at: 0 42 1134
Size: 42 1092
TatI w'GTAC_w
Cuts at: 0 1081 1134
Size: 1081 53
Tth111I GACn'n_nGTC
Cuts at: 0 293 1134
Size: 293 841
Enzymes that do cut:
AccI AcuI AhdI AlwNI ApoI BanII BbsI BbvCI
BpmI Bpu10I BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BstAPI BstYI BtgI BtsI DraIII
EarI EcoICRI Eco57MI EcoRI Hin4I HincII HindIII MmeI
MspA1I PstI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SpeI TatI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmBI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EaeI EagI EciI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SanDI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SphI SrfI SspI StuI StyI SwaI TaqII
TstI XbaI XcmI XhoI XmaI XmnI ZraI