(Linear) (Six-base) MAPSORT of: KIEE5554.seq Check: 1980 from: 1 to: 1287 KIEE5554.orf With 182 enzymes: SgfI * January 29, 2014 12:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 48 1287 Size: 48 1239 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 239 1287 Size: 239 1048 AhdI GACnn_n'nnGTC Cuts at: 0 652 1287 Size: 652 635 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 35 67 1287 Size: 35 32 1220 Fragments arranged by size: 1220 35 32 AlwNI CAG_nnn'CTG Cuts at: 0 63 73 877 1063 1287 Size: 63 10 804 186 224 Fragments arranged by size: 804 224 186 63 10 ApaI G_GGCC'C Cuts at: 0 375 752 844 1287 Size: 375 377 92 443 Fragments arranged by size: 443 377 375 92 ApoI r'AATT_y Cuts at: 0 917 1287 Size: 917 370 AvaI C'yCGr_G Cuts at: 0 21 985 1133 1287 Size: 21 964 148 154 Fragments arranged by size: 964 154 148 21 BanI G'GyrC_C Cuts at: 0 449 702 1287 Size: 449 253 585 Fragments arranged by size: 585 449 253 BanII G_rGCy'C Cuts at: 0 134 375 752 844 1287 Size: 134 241 377 92 443 Fragments arranged by size: 443 377 241 134 92 BbsI GAAGACnn'nnnn_ Cuts at: 0 338 1287 Size: 338 949 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 214 248 826 860 1287 Size: 214 34 578 34 427 Fragments arranged by size: 578 427 214 34 34 Bme1580I G_kGCm'C Cuts at: 0 375 752 844 1287 Size: 375 377 92 443 Fragments arranged by size: 443 377 375 92 BmrI ACTGGGnnnn_n' Cuts at: 0 403 865 917 1287 Size: 403 462 52 370 Fragments arranged by size: 462 403 370 52 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 93 1287 Size: 93 1194 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 642 1085 1287 Size: 642 443 202 Fragments arranged by size: 642 443 202 BsaI GGTCTCn'nnnn_ Cuts at: 0 289 1287 Size: 289 998 BsaHI Gr'CG_yC Cuts at: 0 827 992 1287 Size: 827 165 295 Fragments arranged by size: 827 295 165 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 674 704 1287 Size: 674 30 583 Fragments arranged by size: 674 583 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1064 1287 Size: 1064 223 BseYI C'CCAG_C Cuts at: 0 95 269 795 1159 1287 Size: 95 174 526 364 128 Fragments arranged by size: 526 364 174 128 95 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 811 1287 Size: 811 476 BsiHKAI G_wGCw'C Cuts at: 0 134 1287 Size: 134 1153 BsmI GAATG_Cn' Cuts at: 0 115 1287 Size: 115 1172 BsmBI CGTCTCn'nnnn_ Cuts at: 0 777 1287 Size: 777 510 Bsp1286I G_dGCh'C Cuts at: 0 134 375 752 844 1287 Size: 134 241 377 92 443 Fragments arranged by size: 443 377 241 134 92 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 48 1081 1287 Size: 48 1033 206 Fragments arranged by size: 1033 206 48 BsrBI CCG'CTC Cuts at: 0 771 1287 Size: 771 516 BsrDI GCAATG_nn' Cuts at: 0 596 813 1287 Size: 596 217 474 Fragments arranged by size: 596 474 217 BsrFI r'CCGG_y Cuts at: 0 676 889 1287 Size: 676 213 398 Fragments arranged by size: 676 398 213 BsrGI T'GTAC_A Cuts at: 0 431 1287 Size: 431 856 BssSI C'ACGA_G Cuts at: 0 1258 1287 Size: 1258 29 Bsu36I CC'TnA_GG Cuts at: 0 941 1287 Size: 941 346 BtgI C'CryG_G Cuts at: 0 822 1287 Size: 822 465 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 366 1287 Size: 366 921 BtsI GCAGTG_nn' Cuts at: 0 881 1287 Size: 881 406 DraIII CAC_nnn'GTG Cuts at: 0 197 709 1242 1287 Size: 197 512 533 45 Fragments arranged by size: 533 512 197 45 EarI CTCTTCn'nnn_ Cuts at: 0 436 510 1287 Size: 436 74 777 Fragments arranged by size: 777 436 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 663 1287 Size: 663 624 EcoICRI GAG'CTC Cuts at: 0 132 1287 Size: 132 1155 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 239 642 1085 1287 Size: 239 403 443 202 Fragments arranged by size: 443 403 239 202 EcoO109I rG'GnC_Cy Cuts at: 0 229 266 371 372 728 748 749 840 Size: 229 37 105 1 356 20 1 91 Cuts at: 840 841 1287 Size: 1 446 Fragments arranged by size: 446 356 229 105 91 37 20 1 1 1 EcoRI G'AATT_C Cuts at: 0 917 1287 Size: 917 370 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 55 1287 Size: 55 1232 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 457 489 638 670 1099 1131 1237 1269 Size: 457 32 149 32 429 32 106 32 Cuts at: 1269 1287 Size: 18 Fragments arranged by size: 457 429 149 106 32 32 32 32 18 HindIII A'AGCT_T Cuts at: 0 899 1287 Size: 899 388 MslI CAynn'nnrTG Cuts at: 0 763 806 1287 Size: 763 43 481 Fragments arranged by size: 763 481 43 MspA1I CmG'CkG Cuts at: 0 99 587 795 1287 Size: 99 488 208 492 Fragments arranged by size: 492 488 208 99 NaeI GCC'GGC Cuts at: 0 891 1287 Size: 891 396 NgoMIV G'CCGG_C Cuts at: 0 889 1287 Size: 889 398 NspI r_CATG'y Cuts at: 0 280 1287 Size: 280 1007 PasI CC'CwG_GG Cuts at: 0 743 1030 1287 Size: 743 287 257 Fragments arranged by size: 743 287 257 PflMI CCAn_nnn'nTGG Cuts at: 0 393 482 587 1242 1287 Size: 393 89 105 655 45 Fragments arranged by size: 655 393 105 89 45 PfoI T'CCnGG_A Cuts at: 0 927 1287 Size: 927 360 PpuMI rG'GwC_Cy Cuts at: 0 229 728 1287 Size: 229 499 559 Fragments arranged by size: 559 499 229 PshAI GACnn'nnGTC Cuts at: 0 359 1091 1142 1287 Size: 359 732 51 145 Fragments arranged by size: 732 359 145 51 PspOMI G'GGCC_C Cuts at: 0 371 748 840 1287 Size: 371 377 92 447 Fragments arranged by size: 447 377 371 92 PstI C_TGCA'G Cuts at: 0 59 237 1227 1287 Size: 59 178 990 60 Fragments arranged by size: 990 178 60 59 PvuII CAG'CTG Cuts at: 0 99 587 795 1287 Size: 99 488 208 492 Fragments arranged by size: 492 488 208 99 SacI G_AGCT'C Cuts at: 0 134 1287 Size: 134 1153 SanDI GG'GwC_CC Cuts at: 0 728 1287 Size: 728 559 SapI GCTCTTCn'nnn_ Cuts at: 0 436 510 1287 Size: 436 74 777 Fragments arranged by size: 777 436 74 SbfI CC_TGCA'GG Cuts at: 0 59 1287 Size: 59 1228 SexAI A'CCwGG_T Cuts at: 0 193 705 1287 Size: 193 512 582 Fragments arranged by size: 582 512 193 SfcI C'TryA_G Cuts at: 0 55 233 339 1223 1287 Size: 55 178 106 884 64 Fragments arranged by size: 884 178 106 64 55 SmaI CCC'GGG Cuts at: 0 23 1287 Size: 23 1264 SmlI C'TyrA_G Cuts at: 0 985 1133 1287 Size: 985 148 154 Fragments arranged by size: 985 154 148 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 135 1287 Size: 135 1152 TatI w'GTAC_w Cuts at: 0 431 1287 Size: 431 856 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 674 706 1287 Size: 674 32 581 Fragments arranged by size: 674 581 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 198 856 1287 Size: 198 658 431 Fragments arranged by size: 658 431 198 XhoI C'TCGA_G Cuts at: 0 985 1133 1287 Size: 985 148 154 Fragments arranged by size: 985 154 148 XmaI C'CCGG_G Cuts at: 0 21 1287 Size: 21 1266 Enzymes that do cut: AarI AcuI AhdI AloI AlwNI ApaI ApoI AvaI BanI BanII BbsI BcgI Bme1580I BmrI BplI BpmI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI Bsu36I BtgI BtgZI BtsI DraIII EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I HindIII MslI MspA1I NaeI NgoMIV NspI PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SapI SbfI SexAI SfcI SmaI SmlI TaqII TatI TstI XcmI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BspHI BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I ClaI CspCI DraI DrdI EaeI EagI EcoNI EcoRV FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MmeI MscI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI Tth111I XbaI XmnI ZraI