Restriction map for
pFN21AE5549
(Linear) (Six-base) MAPSORT of: KIEE5549.seq Check: 8207 from: 1 to: 1200
KIEE5549.orf
With 182 enzymes: SgfI *
January 29, 2014 12:05 ..
AclI AA'CG_TT
Cuts at: 0 815 1200
Size: 815 385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 634 1200
Size: 158 476 566
Fragments arranged by size:
566 476 158
ApaI G_GGCC'C
Cuts at: 0 24 1200
Size: 24 1176
ApoI r'AATT_y
Cuts at: 0 163 399 1200
Size: 163 236 801
Fragments arranged by size:
801 236 163
AseI AT'TA_AT
Cuts at: 0 133 1200
Size: 133 1067
BanII G_rGCy'C
Cuts at: 0 24 51 420 1200
Size: 24 27 369 780
Fragments arranged by size:
780 369 27 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 652 1200
Size: 652 548
BfrBI ATG'CAT
Cuts at: 0 568 1200
Size: 568 632
BlpI GC'TnA_GC
Cuts at: 0 104 1200
Size: 104 1096
Bme1580I G_kGCm'C
Cuts at: 0 24 1200
Size: 24 1176
BmrI ACTGGGnnnn_n'
Cuts at: 0 900 1200
Size: 900 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 1200
Size: 625 575
BsaI GGTCTCn'nnnn_
Cuts at: 0 310 1200
Size: 310 890
BsaWI w'CCGG_w
Cuts at: 0 878 1200
Size: 878 322
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 1200
Size: 737 463
BsiEI CG_ry'CG
Cuts at: 0 18 1200
Size: 18 1182
BsiHKAI G_wGCw'C
Cuts at: 0 51 420 1200
Size: 51 369 780
Fragments arranged by size:
780 369 51
BsmI GAATG_Cn'
Cuts at: 0 159 681 1200
Size: 159 522 519
Fragments arranged by size:
522 519 159
Bsp1286I G_dGCh'C
Cuts at: 0 24 51 420 1200
Size: 24 27 369 780
Fragments arranged by size:
780 369 27 24
BsrDI GCAATG_nn'
Cuts at: 0 104 1200
Size: 104 1096
BtsI GCAGTG_nn'
Cuts at: 0 998 1200
Size: 998 202
DraIII CAC_nnn'GTG
Cuts at: 0 466 1200
Size: 466 734
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 466 1200
Size: 466 734
EcoICRI GAG'CTC
Cuts at: 0 49 418 1200
Size: 49 369 782
Fragments arranged by size:
782 369 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 625 634 1200
Size: 158 467 9 566
Fragments arranged by size:
566 467 158 9
EcoO109I rG'GnC_Cy
Cuts at: 0 21 38 1200
Size: 21 17 1162
Fragments arranged by size:
1162 21 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1057 1200
Size: 1057 143
HincII GTy'rAC
Cuts at: 0 737 1200
Size: 737 463
HindIII A'AGCT_T
Cuts at: 0 865 1154 1200
Size: 865 289 46
Fragments arranged by size:
865 289 46
MfeI C'AATT_G
Cuts at: 0 481 1200
Size: 481 719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1200
Size: 481 719
MspA1I CmG'CkG
Cuts at: 0 1006 1200
Size: 1006 194
NsiI A_TGCA'T
Cuts at: 0 570 1200
Size: 570 630
PflMI CCAn_nnn'nTGG
Cuts at: 0 466 1200
Size: 466 734
PspOMI G'GGCC_C
Cuts at: 0 20 1200
Size: 20 1180
PstI C_TGCA'G
Cuts at: 0 838 1005 1200
Size: 838 167 195
Fragments arranged by size:
838 195 167
PvuI CG_AT'CG
Cuts at: 0 18 1200
Size: 18 1182
PvuII CAG'CTG
Cuts at: 0 1006 1200
Size: 1006 194
SacI G_AGCT'C
Cuts at: 0 51 420 1200
Size: 51 369 780
Fragments arranged by size:
780 369 51
SfcI C'TryA_G
Cuts at: 0 834 1001 1200
Size: 834 167 199
Fragments arranged by size:
834 199 167
TatI w'GTAC_w
Cuts at: 0 573 1200
Size: 573 627
XmnI GAAnn'nnTTC
Cuts at: 0 245 797 1200
Size: 245 552 403
Fragments arranged by size:
552 403 245
Enzymes that do cut:
AclI AcuI ApaI ApoI AseI BanII BbsI BfrBI
BlpI Bme1580I BmrI BpmI BsaI BsaWI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BsrDI BtsI DraIII EciI EcoICRI
Eco57MI EcoO109I FalI HincII HindIII MfeI MmeI MspA1I
NsiI PflMI PspOMI PstI PvuI PvuII SacI SfcI
TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BglI BglII BmgBI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DrdI EaeI EagI
EarI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
Hin4I HpaI KasI KpnI MluI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI ZraI