Restriction map for
pFN21AE5548
(Linear) (Six-base) MAPSORT of: KIEE5548.seq Check: 3978 from: 1 to: 1200
KIEE5548.orf
With 182 enzymes: SgfI *
January 29, 2014 12:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 911 1200
Size: 911 289
AflIII A'CryG_T
Cuts at: 0 527 1200
Size: 527 673
AhdI GACnn_n'nnGTC
Cuts at: 0 1191 1200
Size: 1191 9
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 809 841 1200
Size: 809 32 359
Fragments arranged by size:
809 359 32
AlwNI CAG_nnn'CTG
Cuts at: 0 111 1200
Size: 111 1089
AvaI C'yCGr_G
Cuts at: 0 347 1200
Size: 347 853
BanII G_rGCy'C
Cuts at: 0 563 1200
Size: 563 637
BbsI GAAGACnn'nnnn_
Cuts at: 0 407 862 1200
Size: 407 455 338
Fragments arranged by size:
455 407 338
BciVI GTATCCnnnnn_n'
Cuts at: 0 103 1200
Size: 103 1097
BclI T'GATC_A
Cuts at: 0 543 1200
Size: 543 657
BglII A'GATC_T
Cuts at: 0 446 1200
Size: 446 754
BlpI GC'TnA_GC
Cuts at: 0 935 1200
Size: 935 265
Bme1580I G_kGCm'C
Cuts at: 0 618 645 956 1200
Size: 618 27 311 244
Fragments arranged by size:
618 311 244 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 393 1200
Size: 393 807
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 254 1200
Size: 254 946
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1134 1200
Size: 1012 122 66
Fragments arranged by size:
1012 122 66
Bpu10I CC'TnA_GC
Cuts at: 0 125 1200
Size: 125 1075
BsaI GGTCTCn'nnnn_
Cuts at: 0 515 1200
Size: 515 685
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1200
Size: 329 871
BsiHKAI G_wGCw'C
Cuts at: 0 563 1162 1200
Size: 563 599 38
Fragments arranged by size:
599 563 38
BsmI GAATG_Cn'
Cuts at: 0 106 1200
Size: 106 1094
Bsp1286I G_dGCh'C
Cuts at: 0 563 618 645 956 1162 1200
Size: 563 55 27 311 206 38
Fragments arranged by size:
563 311 206 55 38 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 911 1200
Size: 911 289
BsrDI GCAATG_nn'
Cuts at: 0 612 1200
Size: 612 588
BsrGI T'GTAC_A
Cuts at: 0 941 1200
Size: 941 259
BssSI C'ACGA_G
Cuts at: 0 1082 1200
Size: 1082 118
BstEII G'GTnAC_C
Cuts at: 0 255 1200
Size: 255 945
BstXI CCAn_nnnn'nTGG
Cuts at: 0 601 630 1036 1200
Size: 601 29 406 164
Fragments arranged by size:
601 406 164 29
BstYI r'GATC_y
Cuts at: 0 411 446 1200
Size: 411 35 754
Fragments arranged by size:
754 411 35
Bsu36I CC'TnA_GG
Cuts at: 0 149 1125 1200
Size: 149 976 75
Fragments arranged by size:
976 149 75
BtgI C'CryG_G
Cuts at: 0 702 1200
Size: 702 498
BtsI GCAGTG_nn'
Cuts at: 0 645 1200
Size: 645 555
EaeI y'GGCC_r
Cuts at: 0 837 1200
Size: 837 363
EcoICRI GAG'CTC
Cuts at: 0 561 1200
Size: 561 639
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1134 1200
Size: 1012 122 66
Fragments arranged by size:
1012 122 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 148 1200
Size: 148 1052
EcoO109I rG'GnC_Cy
Cuts at: 0 694 1200
Size: 694 506
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 779 811 1177 1200
Size: 779 32 366 23
Fragments arranged by size:
779 366 32 23
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1200
Size: 674 526
MscI TGG'CCA
Cuts at: 0 839 1200
Size: 839 361
MslI CAynn'nnrTG
Cuts at: 0 526 599 1200
Size: 526 73 601
Fragments arranged by size:
601 526 73
MspA1I CmG'CkG
Cuts at: 0 313 476 744 939 1200
Size: 313 163 268 195 261
Fragments arranged by size:
313 268 261 195 163
NcoI C'CATG_G
Cuts at: 0 702 1200
Size: 702 498
NdeI CA'TA_TG
Cuts at: 0 354 1200
Size: 354 846
NspI r_CATG'y
Cuts at: 0 531 1200
Size: 531 669
PciI A'CATG_T
Cuts at: 0 527 1200
Size: 527 673
PflMI CCAn_nnn'nTGG
Cuts at: 0 383 633 708 835 1200
Size: 383 250 75 127 365
Fragments arranged by size:
383 365 250 127 75
PstI C_TGCA'G
Cuts at: 0 749 1200
Size: 749 451
PvuII CAG'CTG
Cuts at: 0 313 744 939 1200
Size: 313 431 195 261
Fragments arranged by size:
431 313 261 195
SacI G_AGCT'C
Cuts at: 0 563 1200
Size: 563 637
ScaI AGT'ACT
Cuts at: 0 382 848 1200
Size: 382 466 352
Fragments arranged by size:
466 382 352
SfcI C'TryA_G
Cuts at: 0 462 745 1200
Size: 462 283 455
Fragments arranged by size:
462 455 283
StyI C'CwwG_G
Cuts at: 0 119 702 991 1200
Size: 119 583 289 209
Fragments arranged by size:
583 289 209 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1081 1200
Size: 1081 119
TatI w'GTAC_w
Cuts at: 0 380 846 941 1095 1200
Size: 380 466 95 154 105
Fragments arranged by size:
466 380 154 105 95
XbaI T'CTAG_A
Cuts at: 0 1137 1200
Size: 1137 63
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 630 684 924 1200
Size: 630 54 240 276
Fragments arranged by size:
630 276 240 54
Enzymes that do cut:
AarI AflIII AhdI AloI AlwNI AvaI BanII BbsI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BsaI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI
EaeI EcoICRI Eco57MI EcoNI EcoO109I Hin4I MmeI MscI
MslI MspA1I NcoI NdeI NspI PciI PflMI PstI
PvuII SacI ScaI SfcI StyI TaqII TatI XbaI
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BssHII BstAPI BstBI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XhoI
XmaI XmnI ZraI