(Linear) (Six-base) MAPSORT of: KIEE5546.seq Check: 3938 from: 1 to: 1167 KIEE5546.orf With 182 enzymes: SgfI * December 5, 2014 13:02 .. AflIII A'CryG_T Cuts at: 0 839 1167 Size: 839 328 AlwNI CAG_nnn'CTG Cuts at: 0 579 1143 1167 Size: 579 564 24 Fragments arranged by size: 579 564 24 ApaI G_GGCC'C Cuts at: 0 49 203 1167 Size: 49 154 964 Fragments arranged by size: 964 154 49 ApaLI G'TGCA_C Cuts at: 0 166 706 1167 Size: 166 540 461 Fragments arranged by size: 540 461 166 AscI GG'CGCG_CC Cuts at: 0 60 1167 Size: 60 1107 AvaI C'yCGr_G Cuts at: 0 48 1167 Size: 48 1119 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 551 584 1167 Size: 551 33 583 Fragments arranged by size: 583 551 33 BanI G'GyrC_C Cuts at: 0 267 658 1167 Size: 267 391 509 Fragments arranged by size: 509 391 267 BanII G_rGCy'C Cuts at: 0 49 203 626 864 887 974 1167 Size: 49 154 423 238 23 87 193 Fragments arranged by size: 423 238 193 154 87 49 23 BciVI GTATCCnnnnn_n' Cuts at: 0 579 1167 Size: 579 588 BglI GCCn_nnn'nGGC Cuts at: 0 53 1167 Size: 53 1114 Bme1580I G_kGCm'C Cuts at: 0 49 170 203 541 710 1167 Size: 49 121 33 338 169 457 Fragments arranged by size: 457 338 169 121 49 33 BmrI ACTGGGnnnn_n' Cuts at: 0 567 1167 Size: 567 600 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 516 1167 Size: 516 651 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 718 1132 1167 Size: 718 414 35 Fragments arranged by size: 718 414 35 BsaI GGTCTCn'nnnn_ Cuts at: 0 292 1167 Size: 292 875 BsaAI yAC'GTr Cuts at: 0 840 1167 Size: 840 327 BsaWI w'CCGG_w Cuts at: 0 5 1167 Size: 5 1162 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 97 127 1167 Size: 97 30 1040 Fragments arranged by size: 1040 97 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 193 235 1167 Size: 193 42 932 Fragments arranged by size: 932 193 42 BseYI C'CCAG_C Cuts at: 0 394 626 1167 Size: 394 232 541 Fragments arranged by size: 541 394 232 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 259 1167 Size: 259 908 BsiHKAI G_wGCw'C Cuts at: 0 170 710 887 1167 Size: 170 540 177 280 Fragments arranged by size: 540 280 177 170 BsiWI C'GTAC_G Cuts at: 0 866 1167 Size: 866 301 BsmI GAATG_Cn' Cuts at: 0 415 1167 Size: 415 752 Bsp1286I G_dGCh'C Cuts at: 0 49 170 203 541 626 710 864 887 Size: 49 121 33 338 85 84 154 23 Cuts at: 887 974 1167 Size: 87 193 Fragments arranged by size: 338 193 154 121 87 85 84 49 33 23 BspEI T'CCGG_A Cuts at: 0 5 1167 Size: 5 1162 BspHI T'CATG_A Cuts at: 0 794 1167 Size: 794 373 BsrDI GCAATG_nn' Cuts at: 0 345 1167 Size: 345 822 BsrFI r'CCGG_y Cuts at: 0 833 1167 Size: 833 334 BssHII G'CGCG_C Cuts at: 0 60 1167 Size: 60 1107 BssSI C'ACGA_G Cuts at: 0 445 1167 Size: 445 722 BstEII G'GTnAC_C Cuts at: 0 465 1167 Size: 465 702 BstXI CCAn_nnnn'nTGG Cuts at: 0 1059 1101 1167 Size: 1059 42 66 Fragments arranged by size: 1059 66 42 BtgI C'CryG_G Cuts at: 0 140 1167 Size: 140 1027 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 95 720 1167 Size: 95 625 447 Fragments arranged by size: 625 447 95 DrdI GACnn_nn'nnGTC Cuts at: 0 760 1033 1167 Size: 760 273 134 Fragments arranged by size: 760 273 134 EaeI y'GGCC_r Cuts at: 0 108 363 1167 Size: 108 255 804 Fragments arranged by size: 804 255 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 564 1167 Size: 564 603 EcoICRI GAG'CTC Cuts at: 0 885 1167 Size: 885 282 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 718 1132 1167 Size: 718 414 35 Fragments arranged by size: 718 414 35 EcoNI CCTnn'n_nnAGG Cuts at: 0 700 1167 Size: 700 467 EcoO109I rG'GnC_Cy Cuts at: 0 199 200 725 1167 Size: 199 1 525 442 Fragments arranged by size: 525 442 199 1 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 140 980 1040 1167 Size: 140 840 60 127 Fragments arranged by size: 840 140 127 60 MslI CAynn'nnrTG Cuts at: 0 173 338 649 1167 Size: 173 165 311 518 Fragments arranged by size: 518 311 173 165 MspA1I CmG'CkG Cuts at: 0 123 274 1041 1167 Size: 123 151 767 126 Fragments arranged by size: 767 151 126 123 NcoI C'CATG_G Cuts at: 0 140 1167 Size: 140 1027 NspI r_CATG'y Cuts at: 0 459 688 1167 Size: 459 229 479 Fragments arranged by size: 479 459 229 PasI CC'CwG_GG Cuts at: 0 1143 1167 Size: 1143 24 PflMI CCAn_nnn'nTGG Cuts at: 0 251 1167 Size: 251 916 PfoI T'CCnGG_A Cuts at: 0 695 1109 1167 Size: 695 414 58 Fragments arranged by size: 695 414 58 PspOMI G'GGCC_C Cuts at: 0 45 199 1167 Size: 45 154 968 Fragments arranged by size: 968 154 45 PstI C_TGCA'G Cuts at: 0 27 584 1167 Size: 27 557 583 Fragments arranged by size: 583 557 27 PvuII CAG'CTG Cuts at: 0 123 1167 Size: 123 1044 SacI G_AGCT'C Cuts at: 0 887 1167 Size: 887 280 SexAI A'CCwGG_T Cuts at: 0 908 1167 Size: 908 259 SfcI C'TryA_G Cuts at: 0 23 356 580 1167 Size: 23 333 224 587 Fragments arranged by size: 587 333 224 23 SmaI CCC'GGG Cuts at: 0 50 1167 Size: 50 1117 SphI G_CATG'C Cuts at: 0 459 1167 Size: 459 708 StyI C'CwwG_G Cuts at: 0 140 1094 1167 Size: 140 954 73 Fragments arranged by size: 954 140 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 133 243 1167 Size: 133 110 924 Fragments arranged by size: 924 133 110 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 898 930 1167 Size: 898 32 237 Fragments arranged by size: 898 237 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 252 444 610 1167 Size: 252 192 166 557 Fragments arranged by size: 557 252 192 166 XmaI C'CCGG_G Cuts at: 0 48 1167 Size: 48 1119 Enzymes that do cut: AflIII AlwNI ApaI ApaLI AscI AvaI BaeI BanI BanII BciVI BglI Bme1580I BmrI BplI BpmI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BsrDI BsrFI BssHII BssSI BstEII BstXI BtgI BtgZI DrdI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I MmeI MslI MspA1I NcoI NspI PasI PflMI PfoI PspOMI PstI PvuII SacI SexAI SfcI SmaI SphI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApoI AseI AsiSI AvrII BamHI BbeI BbsI BbvCI BcgI BclI BfrBI BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaBI BsaHI BsiEI BsmBI BspMI BsrBI BsrGI BstAPI BstBI BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EagI EarI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI XmnI ZraI