Restriction map for
pFN21AE5545
(Linear) (Six-base) MAPSORT of: KIEE5545.seq Check: 9617 from: 1 to: 1164
KIEE5545.orf
With 182 enzymes: SgfI *
January 29, 2014 12:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 407 1164
Size: 407 757
AflIII A'CryG_T
Cuts at: 0 326 1164
Size: 326 838
AlwNI CAG_nnn'CTG
Cuts at: 0 10 597 621 999 1164
Size: 10 587 24 378 165
Fragments arranged by size:
587 378 165 24 10
ApaI G_GGCC'C
Cuts at: 0 58 1164
Size: 58 1106
AvaI C'yCGr_G
Cuts at: 0 44 130 406 547 905 984 1132 1164
Size: 44 86 276 141 358 79 148 32
Fragments arranged by size:
358 276 148 141 86 79 44 32
BanI G'GyrC_C
Cuts at: 0 1041 1164
Size: 1041 123
BanII G_rGCy'C
Cuts at: 0 58 130 164 635 1139 1164
Size: 58 72 34 471 504 25
Fragments arranged by size:
504 471 72 58 34 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 660 1164
Size: 660 504
BglI GCCn_nnn'nGGC
Cuts at: 0 187 1105 1164
Size: 187 918 59
Fragments arranged by size:
918 187 59
BglII A'GATC_T
Cuts at: 0 320 1164
Size: 320 844
BlpI GC'TnA_GC
Cuts at: 0 565 781 1164
Size: 565 216 383
Fragments arranged by size:
565 383 216
Bme1580I G_kGCm'C
Cuts at: 0 58 895 1046 1164
Size: 58 837 151 118
Fragments arranged by size:
837 151 118 58
BmrI ACTGGGnnnn_n'
Cuts at: 0 233 292 1164
Size: 233 59 872
Fragments arranged by size:
872 233 59
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 192 1134 1164
Size: 192 942 30
Fragments arranged by size:
942 192 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 619 1105 1164
Size: 49 570 486 59
Fragments arranged by size:
570 486 59 49
Bpu10I CC'TnA_GC
Cuts at: 0 757 1164
Size: 757 407
BsaI GGTCTCn'nnnn_
Cuts at: 0 565 1164
Size: 565 599
BsaAI yAC'GTr
Cuts at: 0 561 1164
Size: 561 603
BsaHI Gr'CG_yC
Cuts at: 0 254 1164
Size: 254 910
BsaWI w'CCGG_w
Cuts at: 0 422 1164
Size: 422 742
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 403 433 1121 1151 1164
Size: 403 30 688 30 13
Fragments arranged by size:
688 403 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 424 427 565 748 832 1164
Size: 424 3 138 183 84 332
Fragments arranged by size:
424 332 183 138 84 3
BseYI C'CCAG_C
Cuts at: 0 357 584 915 1164
Size: 357 227 331 249
Fragments arranged by size:
357 331 249 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 1164
Size: 385 779
BsiHKAI G_wGCw'C
Cuts at: 0 1139 1164
Size: 1139 25
Bsp1286I G_dGCh'C
Cuts at: 0 58 130 164 635 895 1046 1139 1164
Size: 58 72 34 471 260 151 93 25
Fragments arranged by size:
471 260 151 93 72 58 34 25
BspHI T'CATG_A
Cuts at: 0 314 1164
Size: 314 850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 407 1164
Size: 407 757
BsrBI CCG'CTC
Cuts at: 0 219 777 912 1164
Size: 219 558 135 252
Fragments arranged by size:
558 252 219 135
BsrDI GCAATG_nn'
Cuts at: 0 371 1164
Size: 371 793
BssHII G'CGCG_C
Cuts at: 0 639 1164
Size: 639 525
BssSI C'ACGA_G
Cuts at: 0 484 1065 1164
Size: 484 581 99
Fragments arranged by size:
581 484 99
BstAPI GCAn_nnn'nTGC
Cuts at: 0 10 467 1164
Size: 10 457 697
Fragments arranged by size:
697 457 10
BstBI TT'CG_AA
Cuts at: 0 698 1164
Size: 698 466
BstXI CCAn_nnnn'nTGG
Cuts at: 0 384 1164
Size: 384 780
BstYI r'GATC_y
Cuts at: 0 320 1164
Size: 320 844
Bsu36I CC'TnA_GG
Cuts at: 0 235 1164
Size: 235 929
BtgI C'CryG_G
Cuts at: 0 1100 1164
Size: 1100 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 47 1164
Size: 47 1117
EaeI y'GGCC_r
Cuts at: 0 302 1164
Size: 302 862
EcoICRI GAG'CTC
Cuts at: 0 1137 1164
Size: 1137 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 619 1105 1164
Size: 49 570 486 59
Fragments arranged by size:
570 486 59 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 238 886 1164
Size: 238 648 278
Fragments arranged by size:
648 278 238
EcoO109I rG'GnC_Cy
Cuts at: 0 54 55 135 479 543 945 1164
Size: 54 1 80 344 64 402 219
Fragments arranged by size:
402 344 219 80 64 54 1
FspI TGC'GCA
Cuts at: 0 835 1164
Size: 835 329
HaeII r_GCGC'y
Cuts at: 0 663 1164
Size: 663 501
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1126 1158 1164
Size: 1126 32 6
Fragments arranged by size:
1126 32 6
MslI CAynn'nnrTG
Cuts at: 0 1078 1164
Size: 1078 86
MspA1I CmG'CkG
Cuts at: 0 10 122 249 618 825 1102 1164
Size: 10 112 127 369 207 277 62
Fragments arranged by size:
369 277 207 127 112 62 10
PasI CC'CwG_GG
Cuts at: 0 139 1164
Size: 139 1025
PflMI CCAn_nnn'nTGG
Cuts at: 0 597 1164
Size: 597 567
PfoI T'CCnGG_A
Cuts at: 0 938 1164
Size: 938 226
PpuMI rG'GwC_Cy
Cuts at: 0 479 543 1164
Size: 479 64 621
Fragments arranged by size:
621 479 64
PspOMI G'GGCC_C
Cuts at: 0 54 1164
Size: 54 1110
PstI C_TGCA'G
Cuts at: 0 440 890 953 1164
Size: 440 450 63 211
Fragments arranged by size:
450 440 211 63
PvuII CAG'CTG
Cuts at: 0 618 1164
Size: 618 546
SacI G_AGCT'C
Cuts at: 0 1139 1164
Size: 1139 25
SacII CC_GC'GG
Cuts at: 0 1103 1164
Size: 1103 61
SanDI GG'GwC_CC
Cuts at: 0 479 1164
Size: 479 685
SbfI CC_TGCA'GG
Cuts at: 0 890 1164
Size: 890 274
SexAI A'CCwGG_T
Cuts at: 0 83 1164
Size: 83 1081
SfcI C'TryA_G
Cuts at: 0 436 886 949 1164
Size: 436 450 63 215
Fragments arranged by size:
450 436 215 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1105 1164
Size: 1105 59
SmaI CCC'GGG
Cuts at: 0 46 1164
Size: 46 1118
StyI C'CwwG_G
Cuts at: 0 578 797 1164
Size: 578 219 367
Fragments arranged by size:
578 367 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 349 1164
Size: 349 815
TatI w'GTAC_w
Cuts at: 0 203 839 1164
Size: 203 636 325
Fragments arranged by size:
636 325 203
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 297 1164
Size: 297 867
XmaI C'CCGG_G
Cuts at: 0 44 1164
Size: 44 1120
Enzymes that do cut:
AarI AflIII AlwNI ApaI AvaI BanI BanII BbsI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI EaeI
EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I MslI
MspA1I PasI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SacII SanDI SbfI SexAI SfcI SfiI SmaI
StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BmgBI BmtI BpuEI BsaBI BsiEI BsiWI
BsmI BsmBI BspEI BsrFI BsrGI BstEII BstZ17I BtsI
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SapI ScaI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI XmnI ZraI