Restriction map for
pFN21AE5539
(Linear) (Six-base) MAPSORT of: KIEE5539.seq Check: 1517 from: 1 to: 1119
KIEE5539.orf
With 182 enzymes: SgfI *
January 29, 2014 12:04 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 740 786 1119
Size: 740 46 333
Fragments arranged by size:
740 333 46
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 219 250 771 1119
Size: 219 31 521 348
Fragments arranged by size:
521 348 219 31
ApaI G_GGCC'C
Cuts at: 0 426 1119
Size: 426 693
ApoI r'AATT_y
Cuts at: 0 313 451 514 1119
Size: 313 138 63 605
Fragments arranged by size:
605 313 138 63
AvaI C'yCGr_G
Cuts at: 0 1114 1119
Size: 1114 5
BamHI G'GATC_C
Cuts at: 0 726 1119
Size: 726 393
BanII G_rGCy'C
Cuts at: 0 426 835 953 1119
Size: 426 409 118 166
Fragments arranged by size:
426 409 166 118
BbsI GAAGACnn'nnnn_
Cuts at: 0 702 1119
Size: 702 417
BciVI GTATCCnnnnn_n'
Cuts at: 0 282 596 924 1119
Size: 282 314 328 195
Fragments arranged by size:
328 314 282 195
BfrBI ATG'CAT
Cuts at: 0 532 1119
Size: 532 587
BglII A'GATC_T
Cuts at: 0 155 1119
Size: 155 964
Bme1580I G_kGCm'C
Cuts at: 0 426 1119
Size: 426 693
BmrI ACTGGGnnnn_n'
Cuts at: 0 384 1119
Size: 384 735
Bpu10I CC'TnA_GC
Cuts at: 0 185 1119
Size: 185 934
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 1119
Size: 117 1002
BsaI GGTCTCn'nnnn_
Cuts at: 0 727 1119
Size: 727 392
BsaHI Gr'CG_yC
Cuts at: 0 707 1119
Size: 707 412
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 34 49 64 568 598 1119
Size: 19 15 15 15 504 30 521
Fragments arranged by size:
521 504 30 19 15 15 15
BseYI C'CCAG_C
Cuts at: 0 179 464 1119
Size: 179 285 655
Fragments arranged by size:
655 285 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 869 1119
Size: 523 346 250
Fragments arranged by size:
523 346 250
BsiHKAI G_wGCw'C
Cuts at: 0 191 329 1119
Size: 191 138 790
Fragments arranged by size:
790 191 138
BsmI GAATG_Cn'
Cuts at: 0 532 1119
Size: 532 587
Bsp1286I G_dGCh'C
Cuts at: 0 191 329 426 835 953 1119
Size: 191 138 97 409 118 166
Fragments arranged by size:
409 191 166 138 118 97
BsrDI GCAATG_nn'
Cuts at: 0 587 1119
Size: 587 532
BsrGI T'GTAC_A
Cuts at: 0 479 1031 1119
Size: 479 552 88
Fragments arranged by size:
552 479 88
BstEII G'GTnAC_C
Cuts at: 0 810 1119
Size: 810 309
BstYI r'GATC_y
Cuts at: 0 155 726 1119
Size: 155 571 393
Fragments arranged by size:
571 393 155
BtgI C'CryG_G
Cuts at: 0 662 980 1119
Size: 662 318 139
Fragments arranged by size:
662 318 139
DraI TTT'AAA
Cuts at: 0 612 1008 1119
Size: 612 396 111
Fragments arranged by size:
612 396 111
EaeI y'GGCC_r
Cuts at: 0 308 860 872 1119
Size: 308 552 12 247
Fragments arranged by size:
552 308 247 12
EarI CTCTTCn'nnn_
Cuts at: 0 694 1119
Size: 694 425
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 342 1119
Size: 342 777
EcoNI CCTnn'n_nnAGG
Cuts at: 0 699 964 1061 1119
Size: 699 265 97 58
Fragments arranged by size:
699 265 97 58
EcoO109I rG'GnC_Cy
Cuts at: 0 127 137 422 974 1119
Size: 127 10 285 552 145
Fragments arranged by size:
552 285 145 127 10
EcoRI G'AATT_C
Cuts at: 0 313 1119
Size: 313 806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 27 1119
Size: 27 1092
HaeII r_GCGC'y
Cuts at: 0 400 1119
Size: 400 719
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 726 758 772 804 1119
Size: 34 32 660 32 14 32 315
Fragments arranged by size:
660 315 34 32 32 32 14
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 616 1119
Size: 67 549 503
Fragments arranged by size:
549 503 67
MscI TGG'CCA
Cuts at: 0 874 1119
Size: 874 245
MslI CAynn'nnrTG
Cuts at: 0 332 743 1119
Size: 332 411 376
Fragments arranged by size:
411 376 332
MspA1I CmG'CkG
Cuts at: 0 234 1119
Size: 234 885
NcoI C'CATG_G
Cuts at: 0 662 1119
Size: 662 457
NsiI A_TGCA'T
Cuts at: 0 534 1119
Size: 534 585
PfoI T'CCnGG_A
Cuts at: 0 274 694 1088 1119
Size: 274 420 394 31
Fragments arranged by size:
420 394 274 31
PpuMI rG'GwC_Cy
Cuts at: 0 127 1119
Size: 127 992
PsiI TTA'TAA
Cuts at: 0 65 1119
Size: 65 1054
PspOMI G'GGCC_C
Cuts at: 0 422 1119
Size: 422 697
PstI C_TGCA'G
Cuts at: 0 406 1106 1119
Size: 406 700 13
Fragments arranged by size:
700 406 13
PvuII CAG'CTG
Cuts at: 0 234 1119
Size: 234 885
SanDI GG'GwC_CC
Cuts at: 0 127 1119
Size: 127 992
SexAI A'CCwGG_T
Cuts at: 0 262 719 814 1119
Size: 262 457 95 305
Fragments arranged by size:
457 305 262 95
SfcI C'TryA_G
Cuts at: 0 402 1102 1119
Size: 402 700 17
Fragments arranged by size:
700 402 17
SmlI C'TyrA_G
Cuts at: 0 132 1114 1119
Size: 132 982 5
Fragments arranged by size:
982 132 5
SspI AAT'ATT
Cuts at: 0 1012 1119
Size: 1012 107
StyI C'CwwG_G
Cuts at: 0 505 662 1057 1119
Size: 505 157 395 62
Fragments arranged by size:
505 395 157 62
TatI w'GTAC_w
Cuts at: 0 479 1031 1119
Size: 479 552 88
Fragments arranged by size:
552 479 88
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 503 1055 1119
Size: 503 552 64
Fragments arranged by size:
552 503 64
XhoI C'TCGA_G
Cuts at: 0 1114 1119
Size: 1114 5
Enzymes that do cut:
AhdI AlfI ApaI ApoI AvaI BamHI BanII BbsI
BciVI BfrBI BglII Bme1580I BmrI Bpu10I BpuEI BsaI
BsaHI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI
BsrGI BstEII BstYI BtgI DraI EaeI EarI EciI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I MmeI MscI
MslI MspA1I NcoI NsiI PfoI PpuMI PsiI PspOMI
PstI PvuII SanDI SexAI SfcI SmlI SspI StyI
TatI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BclI
BglI BlpI BmgBI BmtI BplI BpmI BsaAI BsaBI
BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EagI
EcoICRI Eco57MI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TaqII TstI Tth111I XbaI XmaI XmnI
ZraI