(Linear) (Six-base) MAPSORT of: KIEE5538.seq  Check: 4686  from: 1  to: 1095



KIEE5538.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:04  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     132     913    1095 
   Size:        132     781     182

  Fragments arranged by size: 

                 781     182     132
 
ApoI r'AATT_y

Cuts at:      0     474     684     987    1095 
   Size:        474     210     303     108

  Fragments arranged by size: 

                 474     303     210     108
 
AvrII C'CTAG_G

Cuts at:      0      27    1095 
   Size:         27    1068
 
BamHI G'GATC_C

Cuts at:      0     452    1095 
   Size:        452     643
 
BanI G'GyrC_C

Cuts at:      0     970    1095 
   Size:        970     125
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      15    1038    1095 
   Size:         15    1023      57

  Fragments arranged by size: 

                1023      57      15
 
Bme1580I G_kGCm'C

Cuts at:      0     973    1095 
   Size:        973     122
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     191     772    1095 
   Size:        191     581     323

  Fragments arranged by size: 

                 581     323     191
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     729    1095 
   Size:        729     366
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     902    1095 
   Size:         31     871     193

  Fragments arranged by size: 

                 871     193      31
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     136    1095 
   Size:        136     959
 
BseYI C'CCAG_C

Cuts at:      0     119     125    1095 
   Size:        119       6     970

  Fragments arranged by size: 

                 970     119       6
 
BsiHKAI G_wGCw'C

Cuts at:      0    1082    1095 
   Size:       1082      13
 
Bsp1286I G_dGCh'C

Cuts at:      0     973    1082    1095 
   Size:        973     109      13

  Fragments arranged by size: 

                 973     109      13
 
BspHI T'CATG_A

Cuts at:      0     797    1095 
   Size:        797     298
 
BsrDI GCAATG_nn'

Cuts at:      0     438    1095 
   Size:        438     657
 
BsrGI T'GTAC_A

Cuts at:      0     551    1095 
   Size:        551     544
 
BssSI C'ACGA_G

Cuts at:      0     490    1095 
   Size:        490     605
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     519    1095 
   Size:        519     576
 
BstYI r'GATC_y

Cuts at:      0     452    1095 
   Size:        452     643
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     793    1095 
   Size:        793     302
 
EarI CTCTTCn'nnn_

Cuts at:      0      99     730    1095 
   Size:         99     631     365

  Fragments arranged by size: 

                 631     365      99
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     902    1095 
   Size:         31     871     193

  Fragments arranged by size: 

                 871     193      31
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     151     468    1095 
   Size:        151     317     627

  Fragments arranged by size: 

                 627     317     151
 
EcoO109I rG'GnC_Cy

Cuts at:      0      64     485    1095 
   Size:         64     421     610

  Fragments arranged by size: 

                 610     421      64
 
EcoRI G'AATT_C

Cuts at:      0     474    1095 
   Size:        474     621
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     572     604     636    1095 
   Size:        572      32      32     459

  Fragments arranged by size: 

                 572     459      32      32
 
HincII GTy'rAC

Cuts at:      0     167     309    1095 
   Size:        167     142     786

  Fragments arranged by size: 

                 786     167     142
 
HindIII A'AGCT_T

Cuts at:      0     691    1095 
   Size:        691     404
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1046    1095 
   Size:       1046      49
 
MslI CAynn'nnrTG

Cuts at:      0     494     796    1095 
   Size:        494     302     299

  Fragments arranged by size: 

                 494     302     299
 
NspI r_CATG'y

Cuts at:      0     597    1095 
   Size:        597     498
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     132    1095 
   Size:        132     963
 
PfoI T'CCnGG_A

Cuts at:      0     455     715    1095 
   Size:        455     260     380

  Fragments arranged by size: 

                 455     380     260
 
PpuMI rG'GwC_Cy

Cuts at:      0      64     485    1095 
   Size:         64     421     610

  Fragments arranged by size: 

                 610     421      64
 
SapI GCTCTTCn'nnn_

Cuts at:      0     730    1095 
   Size:        730     365
 
SexAI A'CCwGG_T

Cuts at:      0     373    1095 
   Size:        373     722
 
StyI C'CwwG_G

Cuts at:      0      27     315    1016    1095 
   Size:         27     288     701      79

  Fragments arranged by size: 

                 701     288      79      27
 
TatI w'GTAC_w

Cuts at:      0     551    1095 
   Size:        551     544
 
Tth111I GACn'n_nGTC

Cuts at:      0     909    1095 
   Size:        909     186
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     963    1095 
   Size:        963     132
 
XmnI GAAnn'nnTTC

Cuts at:      0     724    1095 
   Size:        724     371
 

 Enzymes that do cut:

    AlwNI     ApoI    AvrII    BamHI     BanI     BbsI Bme1580I     BmrI
     BplI     BpmI    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI    BsrDI
    BsrGI    BssSI    BstXI    BstYI     DrdI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRI    Hin4I   HincII  HindIII     MmeI     MslI     NspI
    PflMI     PfoI    PpuMI     SapI    SexAI     StyI     TatI  Tth111I
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI     BaeI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrFI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EaeI
     EagI     EciI  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI     XbaI     XhoI
     XmaI     ZraI