Restriction map for
pFN21AE5537
(Linear) (Six-base) MAPSORT of: KIEE5537.seq Check: 9484 from: 1 to: 1308
KIEE5537.orf
With 182 enzymes: SgfI *
January 29, 2014 12:04 ..
AccI GT'mk_AC
Cuts at: 0 1145 1308
Size: 1145 163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 384 550 1164 1308
Size: 54 330 166 614 144
Fragments arranged by size:
614 330 166 144 54
AlwNI CAG_nnn'CTG
Cuts at: 0 336 818 1308
Size: 336 482 490
Fragments arranged by size:
490 482 336
ApaI G_GGCC'C
Cuts at: 0 884 1308
Size: 884 424
ApoI r'AATT_y
Cuts at: 0 105 485 581 1308
Size: 105 380 96 727
Fragments arranged by size:
727 380 105 96
AvaI C'yCGr_G
Cuts at: 0 166 590 1308
Size: 166 424 718
Fragments arranged by size:
718 424 166
BanI G'GyrC_C
Cuts at: 0 554 1308
Size: 554 754
BanII G_rGCy'C
Cuts at: 0 707 884 1016 1193 1308
Size: 707 177 132 177 115
Fragments arranged by size:
707 177 177 132 115
BbsI GAAGACnn'nnnn_
Cuts at: 0 60 357 1308
Size: 60 297 951
Fragments arranged by size:
951 297 60
Bme1580I G_kGCm'C
Cuts at: 0 10 884 1308
Size: 10 874 424
Fragments arranged by size:
874 424 10
BmgBI CAC'GTC
Cuts at: 0 1259 1308
Size: 1259 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 1158 1211 1308
Size: 1158 53 97
Fragments arranged by size:
1158 97 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 196 307 400 445 563 1030 1308
Size: 35 161 111 93 45 118 467 278
Fragments arranged by size:
467 278 161 118 111 93 45 35
BsaBI GATnn'nnATC
Cuts at: 0 961 1308
Size: 961 347
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 868 898 1054 1084 1308
Size: 868 30 156 30 224
Fragments arranged by size:
868 224 156 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 442 1308
Size: 442 866
BseYI C'CCAG_C
Cuts at: 0 216 226 558 1308
Size: 216 10 332 750
Fragments arranged by size:
750 332 216 10
BsiEI CG_ry'CG
Cuts at: 0 1002 1308
Size: 1002 306
BsiHKAI G_wGCw'C
Cuts at: 0 707 1193 1289 1308
Size: 707 486 96 19
Fragments arranged by size:
707 486 96 19
BsmI GAATG_Cn'
Cuts at: 0 465 687 1308
Size: 465 222 621
Fragments arranged by size:
621 465 222
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1249 1308
Size: 1249 59
Bsp1286I G_dGCh'C
Cuts at: 0 10 707 884 1016 1193 1289 1308
Size: 10 697 177 132 177 96 19
Fragments arranged by size:
697 177 177 132 96 19 10
BspHI T'CATG_A
Cuts at: 0 314 1308
Size: 314 994
BsrFI r'CCGG_y
Cuts at: 0 374 613 1224 1308
Size: 374 239 611 84
Fragments arranged by size:
611 374 239 84
BstAPI GCAn_nnn'nTGC
Cuts at: 0 860 1308
Size: 860 448
BstXI CCAn_nnnn'nTGG
Cuts at: 0 566 1308
Size: 566 742
BstYI r'GATC_y
Cuts at: 0 171 804 1308
Size: 171 633 504
Fragments arranged by size:
633 504 171
BtgI C'CryG_G
Cuts at: 0 44 359 686 936 1080 1244 1308
Size: 44 315 327 250 144 164 64
Fragments arranged by size:
327 315 250 164 144 64 44
EaeI y'GGCC_r
Cuts at: 0 137 999 1127 1226 1308
Size: 137 862 128 99 82
Fragments arranged by size:
862 137 128 99 82
EagI C'GGCC_G
Cuts at: 0 999 1308
Size: 999 309
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 69 774 1308
Size: 69 705 534
Fragments arranged by size:
705 534 69
EcoICRI GAG'CTC
Cuts at: 0 705 1191 1308
Size: 705 486 117
Fragments arranged by size:
705 486 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 54 196 307 384 400 445 550
Size: 35 19 142 111 77 16 45 105
Cuts at: 550 563 1030 1164 1308
Size: 13 467 134 144
Fragments arranged by size:
467 144 142 134 111 105 77 45
35 19 16 13
EcoO109I rG'GnC_Cy
Cuts at: 0 180 881 1308
Size: 180 701 427
Fragments arranged by size:
701 427 180
EcoRI G'AATT_C
Cuts at: 0 581 1308
Size: 581 727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 219 1194 1308
Size: 219 975 114
Fragments arranged by size:
975 219 114
HaeII r_GCGC'y
Cuts at: 0 1270 1308
Size: 1270 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 770 802 1308
Size: 463 32 275 32 506
Fragments arranged by size:
506 463 275 32 32
HincII GTy'rAC
Cuts at: 0 1146 1308
Size: 1146 162
HindIII A'AGCT_T
Cuts at: 0 278 1308
Size: 278 1030
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 1308
Size: 875 433
MscI TGG'CCA
Cuts at: 0 139 1129 1308
Size: 139 990 179
Fragments arranged by size:
990 179 139
MslI CAynn'nnrTG
Cuts at: 0 331 799 1308
Size: 331 468 509
Fragments arranged by size:
509 468 331
NaeI GCC'GGC
Cuts at: 0 376 1226 1308
Size: 376 850 82
Fragments arranged by size:
850 376 82
NcoI C'CATG_G
Cuts at: 0 359 686 936 1080 1308
Size: 359 327 250 144 228
Fragments arranged by size:
359 327 250 228 144
NgoMIV G'CCGG_C
Cuts at: 0 374 1224 1308
Size: 374 850 84
Fragments arranged by size:
850 374 84
PasI CC'CwG_GG
Cuts at: 0 31 1308
Size: 31 1277
PflMI CCAn_nnn'nTGG
Cuts at: 0 198 423 1308
Size: 198 225 885
Fragments arranged by size:
885 225 198
PfoI T'CCnGG_A
Cuts at: 0 12 1193 1308
Size: 12 1181 115
Fragments arranged by size:
1181 115 12
PspOMI G'GGCC_C
Cuts at: 0 880 1308
Size: 880 428
SacI G_AGCT'C
Cuts at: 0 707 1193 1308
Size: 707 486 115
Fragments arranged by size:
707 486 115
SalI G'TCGA_C
Cuts at: 0 1144 1308
Size: 1144 164
SexAI A'CCwGG_T
Cuts at: 0 119 1308
Size: 119 1189
SmaI CCC'GGG
Cuts at: 0 592 1308
Size: 592 716
StuI AGG'CCT
Cuts at: 0 1066 1308
Size: 1066 242
StyI C'CwwG_G
Cuts at: 0 359 686 936 1049 1080 1308
Size: 359 327 250 113 31 228
Fragments arranged by size:
359 327 250 228 113 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 572 619 1308
Size: 572 47 689
Fragments arranged by size:
689 572 47
TatI w'GTAC_w
Cuts at: 0 1091 1308
Size: 1091 217
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 868 900 1223 1255 1308
Size: 868 32 323 32 53
Fragments arranged by size:
868 323 53 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 661 1308
Size: 661 647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 234 1056 1122 1308
Size: 234 822 66 186
Fragments arranged by size:
822 234 186 66
XmaI C'CCGG_G
Cuts at: 0 590 1308
Size: 590 718
XmnI GAAnn'nnTTC
Cuts at: 0 843 1308
Size: 843 465
Enzymes that do cut:
AccI AcuI AlwNI ApaI ApoI AvaI BanI BanII
BbsI Bme1580I BmgBI BmrI BpmI BsaBI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrFI
BstAPI BstXI BstYI BtgI EaeI EagI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
MmeI MscI MslI NaeI NcoI NgoMIV PasI PflMI
PfoI PspOMI SacI SalI SexAI SmaI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsgI BsiWI BspEI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EarI EcoNI EcoRV FseI FspI FspAI HpaI KasI
KpnI MfeI MluI MspA1I NarI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacII SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XhoI ZraI