Restriction map for
pFN21AE5529
(Linear) (Six-base) MAPSORT of: KIEE5529.seq Check: 3869 from: 1 to: 1062
KIEE5529.orf
With 182 enzymes: SgfI *
January 29, 2014 12:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1062
Size: 676 386
AleI CACnn'nnGTG
Cuts at: 0 821 1062
Size: 821 241
AvaI C'yCGr_G
Cuts at: 0 353 1062
Size: 353 709
BbsI GAAGACnn'nnnn_
Cuts at: 0 61 101 976 1062
Size: 61 40 875 86
Fragments arranged by size:
875 86 61 40
BclI T'GATC_A
Cuts at: 0 638 851 1062
Size: 638 213 211
Fragments arranged by size:
638 213 211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 1062
Size: 436 626
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 1062
Size: 115 947
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 845 1062
Size: 845 217
BsiHKAI G_wGCw'C
Cuts at: 0 156 902 1062
Size: 156 746 160
Fragments arranged by size:
746 160 156
BsmI GAATG_Cn'
Cuts at: 0 133 1062
Size: 133 929
Bsp1286I G_dGCh'C
Cuts at: 0 156 902 1062
Size: 156 746 160
Fragments arranged by size:
746 160 156
BsrDI GCAATG_nn'
Cuts at: 0 194 1062
Size: 194 868
BssSI C'ACGA_G
Cuts at: 0 601 1062
Size: 601 461
BstYI r'GATC_y
Cuts at: 0 164 1062
Size: 164 898
BtgI C'CryG_G
Cuts at: 0 80 437 1062
Size: 80 357 625
Fragments arranged by size:
625 357 80
BtsI GCAGTG_nn'
Cuts at: 0 128 1062
Size: 128 934
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 312 347 1062
Size: 312 35 715
Fragments arranged by size:
715 312 35
DraI TTT'AAA
Cuts at: 0 369 1062
Size: 369 693
DrdI GACnn_nn'nnGTC
Cuts at: 0 505 1062
Size: 505 557
EaeI y'GGCC_r
Cuts at: 0 914 1062
Size: 914 148
EarI CTCTTCn'nnn_
Cuts at: 0 883 1062
Size: 883 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 973 1062
Size: 973 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 676 1062
Size: 436 240 386
Fragments arranged by size:
436 386 240
HindIII A'AGCT_T
Cuts at: 0 371 1062
Size: 371 691
MscI TGG'CCA
Cuts at: 0 916 1062
Size: 916 146
MslI CAynn'nnrTG
Cuts at: 0 385 821 1062
Size: 385 436 241
Fragments arranged by size:
436 385 241
MspA1I CmG'CkG
Cuts at: 0 289 1002 1062
Size: 289 713 60
Fragments arranged by size:
713 289 60
NcoI C'CATG_G
Cuts at: 0 80 437 1062
Size: 80 357 625
Fragments arranged by size:
625 357 80
NspI r_CATG'y
Cuts at: 0 403 1062
Size: 403 659
PasI CC'CwG_GG
Cuts at: 0 139 1062
Size: 139 923
PflMI CCAn_nnn'nTGG
Cuts at: 0 662 1062
Size: 662 400
PvuII CAG'CTG
Cuts at: 0 289 1002 1062
Size: 289 713 60
Fragments arranged by size:
713 289 60
SfcI C'TryA_G
Cuts at: 0 903 1062
Size: 903 159
SmaI CCC'GGG
Cuts at: 0 355 1062
Size: 355 707
SphI G_CATG'C
Cuts at: 0 403 1062
Size: 403 659
SspI AAT'ATT
Cuts at: 0 89 921 1062
Size: 89 832 141
Fragments arranged by size:
832 141 89
StyI C'CwwG_G
Cuts at: 0 80 437 661 892 1013 1062
Size: 80 357 224 231 121 49
Fragments arranged by size:
357 231 224 121 80 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 644 1062
Size: 644 418
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 592 624 1062
Size: 592 32 438
Fragments arranged by size:
592 438 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 201 1062
Size: 201 861
XmaI C'CCGG_G
Cuts at: 0 353 1062
Size: 353 709
Enzymes that do cut:
AcuI AleI AvaI BbsI BclI BpmI BseRI BsgI
BsiHKAI BsmI Bsp1286I BsrDI BssSI BstYI BtgI BtsI
CspCI DraI DrdI EaeI EarI EciI Eco57MI HindIII
MscI MslI MspA1I NcoI NspI PasI PflMI PvuII
SfcI SmaI SphI SspI StyI TaqII TstI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
DraIII EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI StuI SwaI TatI Tth111I XbaI
XhoI XmnI ZraI