Restriction map for
pFN21AE5515
(Linear) (Six-base) MAPSORT of: KIEE5515.seq Check: 3836 from: 1 to: 1080
KIEE5515.orf
With 182 enzymes: SgfI *
January 29, 2014 12:04 ..
Acc65I G'GTAC_C
Cuts at: 0 367 1080
Size: 367 713
ApoI r'AATT_y
Cuts at: 0 948 970 1080
Size: 948 22 110
Fragments arranged by size:
948 110 22
AvaI C'yCGr_G
Cuts at: 0 911 1080
Size: 911 169
AvrII C'CTAG_G
Cuts at: 0 429 1080
Size: 429 651
BanI G'GyrC_C
Cuts at: 0 367 1080
Size: 367 713
BglII A'GATC_T
Cuts at: 0 1055 1080
Size: 1055 25
Bme1580I G_kGCm'C
Cuts at: 0 832 1080
Size: 832 248
BmrI ACTGGGnnnn_n'
Cuts at: 0 374 1080
Size: 374 706
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 754 854 1080
Size: 361 393 100 226
Fragments arranged by size:
393 361 226 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 615 1080
Size: 615 465
BsaWI w'CCGG_w
Cuts at: 0 50 1080
Size: 50 1030
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 410 440 718 748 1080
Size: 410 30 278 30 332
Fragments arranged by size:
410 332 278 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 20 971 1080
Size: 20 951 109
Fragments arranged by size:
951 109 20
BsiHKAI G_wGCw'C
Cuts at: 0 221 1080
Size: 221 859
BsmI GAATG_Cn'
Cuts at: 0 325 1080
Size: 325 755
Bsp1286I G_dGCh'C
Cuts at: 0 221 832 1080
Size: 221 611 248
Fragments arranged by size:
611 248 221
BspHI T'CATG_A
Cuts at: 0 264 1080
Size: 264 816
BsrDI GCAATG_nn'
Cuts at: 0 824 1080
Size: 824 256
BstAPI GCAn_nnn'nTGC
Cuts at: 0 14 1080
Size: 14 1066
BstEII G'GTnAC_C
Cuts at: 0 60 1080
Size: 60 1020
BstXI CCAn_nnnn'nTGG
Cuts at: 0 679 1030 1080
Size: 679 351 50
Fragments arranged by size:
679 351 50
BstYI r'GATC_y
Cuts at: 0 1055 1080
Size: 1055 25
BtsI GCAGTG_nn'
Cuts at: 0 221 1080
Size: 221 859
DrdI GACnn_nn'nnGTC
Cuts at: 0 961 1080
Size: 961 119
EaeI y'GGCC_r
Cuts at: 0 72 484 572 1080
Size: 72 412 88 508
Fragments arranged by size:
508 412 88 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 754 854 1080
Size: 361 393 100 226
Fragments arranged by size:
393 361 226 100
EcoRV GAT'ATC
Cuts at: 0 1020 1080
Size: 1020 60
HaeII r_GCGC'y
Cuts at: 0 101 1080
Size: 101 979
HincII GTy'rAC
Cuts at: 0 908 1080
Size: 908 172
KpnI G_GTAC'C
Cuts at: 0 371 1080
Size: 371 709
MfeI C'AATT_G
Cuts at: 0 356 1080
Size: 356 724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1000 1080
Size: 142 858 80
Fragments arranged by size:
858 142 80
MscI TGG'CCA
Cuts at: 0 74 574 1080
Size: 74 500 506
Fragments arranged by size:
506 500 74
MslI CAynn'nnrTG
Cuts at: 0 421 542 677 1028 1080
Size: 421 121 135 351 52
Fragments arranged by size:
421 351 135 121 52
MspA1I CmG'CkG
Cuts at: 0 322 616 1080
Size: 322 294 464
Fragments arranged by size:
464 322 294
NspI r_CATG'y
Cuts at: 0 153 1080
Size: 153 927
PshAI GACnn'nnGTC
Cuts at: 0 698 1080
Size: 698 382
PvuII CAG'CTG
Cuts at: 0 322 616 1080
Size: 322 294 464
Fragments arranged by size:
464 322 294
SfcI C'TryA_G
Cuts at: 0 865 1080
Size: 865 215
StuI AGG'CCT
Cuts at: 0 434 1080
Size: 434 646
StyI C'CwwG_G
Cuts at: 0 65 429 1080
Size: 65 364 651
Fragments arranged by size:
651 364 65
TatI w'GTAC_w
Cuts at: 0 618 1080
Size: 618 462
Enzymes that do cut:
Acc65I ApoI AvaI AvrII BanI BglII Bme1580I BmrI
BpmI BsaI BsaWI BsaXI BseRI BsiHKAI BsmI Bsp1286I
BspHI BsrDI BstAPI BstEII BstXI BstYI BtsI DrdI
EaeI Eco57MI EcoRV HaeII HincII KpnI MfeI MmeI
MscI MslI MspA1I NspI PshAI PvuII SfcI StuI
StyI TatI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI BaeI BamHI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BseYI
BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII EagI EarI EciI EcoICRI
EcoNI EcoO109I EcoRI FalI FseI FspI FspAI Hin4I
HindIII HpaI KasI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI