(Linear) (Six-base) MAPSORT of: KIEE5513.seq  Check: 9991  from: 1  to: 1251



KIEE5513.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:03  ..

AclI AA'CG_TT

Cuts at:      0      92    1251 
   Size:         92    1159
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     490    1251 
   Size:        490     761
 
AlwNI CAG_nnn'CTG

Cuts at:      0     169     835    1251 
   Size:        169     666     416

  Fragments arranged by size: 

                 666     416     169
 
ApoI r'AATT_y

Cuts at:      0     756    1251 
   Size:        756     495
 
AvaI C'yCGr_G

Cuts at:      0    1047    1251 
   Size:       1047     204
 
BanI G'GyrC_C

Cuts at:      0    1027    1251 
   Size:       1027     224
 
BanII G_rGCy'C

Cuts at:      0    1084    1251 
   Size:       1084     167
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     414     448    1251 
   Size:        414      34     803

  Fragments arranged by size: 

                 803     414      34
 
BmgBI CAC'GTC

Cuts at:      0     595    1251 
   Size:        595     656
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1101    1251 
   Size:       1101     150
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     722    1251 
   Size:        722     529
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     838    1192    1251 
   Size:        838     354      59

  Fragments arranged by size: 

                 838     354      59
 
BsaBI GATnn'nnATC

Cuts at:      0     104    1251 
   Size:        104    1147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     295     325     901     931    1251 
   Size:        295      30     576      30     320

  Fragments arranged by size: 

                 576     320     295      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1210    1251 
   Size:       1210      41
 
BseYI C'CCAG_C

Cuts at:      0    1075    1251 
   Size:       1075     176
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1006    1251 
   Size:       1006     245
 
BsiHKAI G_wGCw'C

Cuts at:      0      62     653    1251 
   Size:         62     591     598

  Fragments arranged by size: 

                 598     591      62
 
BsmI GAATG_Cn'

Cuts at:      0     550     987    1251 
   Size:        550     437     264

  Fragments arranged by size: 

                 550     437     264
 
Bsp1286I G_dGCh'C

Cuts at:      0      62     653    1084    1251 
   Size:         62     591     431     167

  Fragments arranged by size: 

                 591     431     167      62
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     831    1198    1251 
   Size:        831     367      53

  Fragments arranged by size: 

                 831     367      53
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     510     855    1251 
   Size:        510     345     396

  Fragments arranged by size: 

                 510     396     345
 
BstYI r'GATC_y

Cuts at:      0     599     724    1178    1251 
   Size:        599     125     454      73

  Fragments arranged by size: 

                 599     454     125      73
 
BtsI GCAGTG_nn'

Cuts at:      0     432    1018    1251 
   Size:        432     586     233

  Fragments arranged by size: 

                 586     432     233
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     637    1251 
   Size:        637     614
 
EaeI y'GGCC_r

Cuts at:      0     131    1251 
   Size:        131    1120
 
EarI CTCTTCn'nnn_

Cuts at:      0     888    1251 
   Size:        888     363
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     490    1251 
   Size:        490     761
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     469    1251 
   Size:        469     782
 
EcoO109I rG'GnC_Cy

Cuts at:      0     852    1251 
   Size:        852     399
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     648    1251 
   Size:        648     603
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     584     616    1251 
   Size:        584      32     635

  Fragments arranged by size: 

                 635     584      32
 
HincII GTy'rAC

Cuts at:      0      90     201    1251 
   Size:         90     111    1050

  Fragments arranged by size: 

                1050     111      90
 
HpaI GTT'AAC

Cuts at:      0      90    1251 
   Size:         90    1161
 
MscI TGG'CCA

Cuts at:      0     133    1251 
   Size:        133    1118
 
MslI CAynn'nnrTG

Cuts at:      0      50      53     344     508    1251 
   Size:         50       3     291     164     743

  Fragments arranged by size: 

                 743     291     164      50       3
 
NdeI CA'TA_TG

Cuts at:      0     945    1041    1251 
   Size:        945      96     210

  Fragments arranged by size: 

                 945     210      96
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     536    1251 
   Size:        536     715
 
PfoI T'CCnGG_A

Cuts at:      0    1235    1251 
   Size:       1235      16
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      64      96     563     595    1251 
   Size:         64      32     467      32     656

  Fragments arranged by size: 

                 656     467      64      32      32
 
SmaI CCC'GGG

Cuts at:      0    1049    1251 
   Size:       1049     202
 
SspI AAT'ATT

Cuts at:      0     657    1251 
   Size:        657     594
 
StyI C'CwwG_G

Cuts at:      0     848    1104    1251 
   Size:        848     256     147

  Fragments arranged by size: 

                 848     256     147
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     730    1251 
   Size:        730     521
 
TatI w'GTAC_w

Cuts at:      0     193     353    1034    1251 
   Size:        193     160     681     217

  Fragments arranged by size: 

                 681     217     193     160
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     390     422     901     933    1251 
   Size:        390      32     479      32     318

  Fragments arranged by size: 

                 479     390     318      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1233    1251 
   Size:       1233      18
 
XmaI C'CCGG_G

Cuts at:      0    1047    1251 
   Size:       1047     204
 
XmnI GAAnn'nnTTC

Cuts at:      0      79    1251 
   Size:         79    1172
 

 Enzymes that do cut:

     AclI     AcuI    AlwNI     ApoI     AvaI     BanI    BanII     BcgI
    BmgBI     BmrI     BplI     BsaI    BsaBI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BstXI    BstYI     BtsI
     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I
   HincII     HpaI     MscI     MslI     NdeI    PflMI     PfoI     PpiI
     SmaI     SspI     StyI    TaqII     TatI     TstI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmtI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     EagI     EciI  EcoICRI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MfeI
     MluI     MmeI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI  Tth111I
     XbaI     XhoI     ZraI