Restriction map for
pFN21AE5512
(Linear) (Six-base) MAPSORT of: KIEE5512.seq Check: 3548 from: 1 to: 1080
KIEE5512.orf
With 182 enzymes: SgfI *
January 29, 2014 12:03 ..
AccI GT'mk_AC
Cuts at: 0 1018 1080
Size: 1018 62
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 320 1080
Size: 218 102 760
Fragments arranged by size:
760 218 102
AlwNI CAG_nnn'CTG
Cuts at: 0 21 1080
Size: 21 1059
ApoI r'AATT_y
Cuts at: 0 682 1080
Size: 682 398
BanI G'GyrC_C
Cuts at: 0 908 1080
Size: 908 172
BanII G_rGCy'C
Cuts at: 0 390 1080
Size: 390 690
BbsI GAAGACnn'nnnn_
Cuts at: 0 195 1080
Size: 195 885
BfrBI ATG'CAT
Cuts at: 0 624 1080
Size: 624 456
BglII A'GATC_T
Cuts at: 0 48 1080
Size: 48 1032
Bme1580I G_kGCm'C
Cuts at: 0 373 911 998 1080
Size: 373 538 87 82
Fragments arranged by size:
538 373 87 82
BmrI ACTGGGnnnn_n'
Cuts at: 0 81 661 1080
Size: 81 580 419
Fragments arranged by size:
580 419 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 901 1080
Size: 387 514 179
Fragments arranged by size:
514 387 179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 1080
Size: 29 1051
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1000 1030 1080
Size: 1000 30 50
Fragments arranged by size:
1000 50 30
BseYI C'CCAG_C
Cuts at: 0 904 1080
Size: 904 176
BsiHKAI G_wGCw'C
Cuts at: 0 935 1013 1080
Size: 935 78 67
Fragments arranged by size:
935 78 67
BsmI GAATG_Cn'
Cuts at: 0 624 1080
Size: 624 456
Bsp1286I G_dGCh'C
Cuts at: 0 373 390 911 935 998 1013 1080
Size: 373 17 521 24 63 15 67
Fragments arranged by size:
521 373 67 63 24 17 15
BsrFI r'CCGG_y
Cuts at: 0 870 1080
Size: 870 210
BstXI CCAn_nnnn'nTGG
Cuts at: 0 165 192 1080
Size: 165 27 888
Fragments arranged by size:
888 165 27
BstYI r'GATC_y
Cuts at: 0 48 1080
Size: 48 1032
DraI TTT'AAA
Cuts at: 0 681 1080
Size: 681 399
EarI CTCTTCn'nnn_
Cuts at: 0 778 934 1080
Size: 778 156 146
Fragments arranged by size:
778 156 146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 320 387 901 1080
Size: 218 102 67 514 179
Fragments arranged by size:
514 218 179 102 67
EcoO109I rG'GnC_Cy
Cuts at: 0 18 1080
Size: 18 1062
HaeII r_GCGC'y
Cuts at: 0 129 1080
Size: 129 951
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 377 409 721 753 1000 1032 1080
Size: 377 32 312 32 247 32 48
Fragments arranged by size:
377 312 247 48 32 32 32
HincII GTy'rAC
Cuts at: 0 447 1080
Size: 447 633
HindIII A'AGCT_T
Cuts at: 0 667 1080
Size: 667 413
MfeI C'AATT_G
Cuts at: 0 301 1080
Size: 301 779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 1080
Size: 357 723
MspA1I CmG'CkG
Cuts at: 0 64 882 1080
Size: 64 818 198
Fragments arranged by size:
818 198 64
NaeI GCC'GGC
Cuts at: 0 872 1080
Size: 872 208
NgoMIV G'CCGG_C
Cuts at: 0 870 1080
Size: 870 210
NsiI A_TGCA'T
Cuts at: 0 626 1080
Size: 626 454
PstI C_TGCA'G
Cuts at: 0 152 1080
Size: 152 928
PvuII CAG'CTG
Cuts at: 0 64 1080
Size: 64 1016
SapI GCTCTTCn'nnn_
Cuts at: 0 934 1080
Size: 934 146
SfcI C'TryA_G
Cuts at: 0 148 795 1080
Size: 148 647 285
Fragments arranged by size:
647 285 148
SmlI C'TyrA_G
Cuts at: 0 44 1080
Size: 44 1036
StuI AGG'CCT
Cuts at: 0 982 1080
Size: 982 98
StyI C'CwwG_G
Cuts at: 0 110 815 855 1080
Size: 110 705 40 225
Fragments arranged by size:
705 225 110 40
TatI w'GTAC_w
Cuts at: 0 89 101 1080
Size: 89 12 979
Fragments arranged by size:
979 89 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 207 239 550 582 1080
Size: 207 32 311 32 498
Fragments arranged by size:
498 311 207 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 268 1080
Size: 268 812
XbaI T'CTAG_A
Cuts at: 0 513 1080
Size: 513 567
Enzymes that do cut:
AccI AcuI AlwNI ApoI BanI BanII BbsI BfrBI
BglII Bme1580I BmrI BpmI BpuEI BsaXI BseYI BsiHKAI
BsmI Bsp1286I BsrFI BstXI BstYI DraI EarI Eco57MI
EcoO109I HaeII Hin4I HincII HindIII MfeI MmeI MspA1I
NaeI NgoMIV NsiI PstI PvuII SapI SfcI SmlI
StuI StyI TatI TstI Tth111I XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BglI BlpI BmgBI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HpaI KasI KpnI MluI MscI MslI NarI
NcoI NdeI NheI NotI NruI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII XcmI XhoI XmaI XmnI ZraI