Restriction map for
pFN21AE5510
(Linear) (Six-base) MAPSORT of: KIEE5510.seq Check: 333 from: 1 to: 1302
KIEE5510.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
AccI GT'mk_AC
Cuts at: 0 563 1302
Size: 563 739
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 700 1045 1302
Size: 208 492 345 257
Fragments arranged by size:
492 345 257 208
AhdI GACnn_n'nnGTC
Cuts at: 0 783 1302
Size: 783 519
AleI CACnn'nnGTG
Cuts at: 0 616 1302
Size: 616 686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1033 1302
Size: 1033 269
AlwNI CAG_nnn'CTG
Cuts at: 0 745 1302
Size: 745 557
ApaI G_GGCC'C
Cuts at: 0 223 1302
Size: 223 1079
ApoI r'AATT_y
Cuts at: 0 274 1302
Size: 274 1028
AvaI C'yCGr_G
Cuts at: 0 23 924 1302
Size: 23 901 378
Fragments arranged by size:
901 378 23
BanI G'GyrC_C
Cuts at: 0 553 1186 1302
Size: 553 633 116
Fragments arranged by size:
633 553 116
BanII G_rGCy'C
Cuts at: 0 223 469 1163 1302
Size: 223 246 694 139
Fragments arranged by size:
694 246 223 139
BbeI G_GCGC'C
Cuts at: 0 557 1190 1302
Size: 557 633 112
Fragments arranged by size:
633 557 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 688 884 1302
Size: 688 196 418
Fragments arranged by size:
688 418 196
BclI T'GATC_A
Cuts at: 0 455 1302
Size: 455 847
BglI GCCn_nnn'nGGC
Cuts at: 0 694 1302
Size: 694 608
BglII A'GATC_T
Cuts at: 0 405 1302
Size: 405 897
Bme1580I G_kGCm'C
Cuts at: 0 107 223 1302
Size: 107 116 1079
Fragments arranged by size:
1079 116 107
BmrI ACTGGGnnnn_n'
Cuts at: 0 101 430 824 909 1302
Size: 101 329 394 85 393
Fragments arranged by size:
394 393 329 101 85
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 673 691 1302
Size: 24 649 18 611
Fragments arranged by size:
649 611 24 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1302
Size: 559 743
BsaI GGTCTCn'nnnn_
Cuts at: 0 873 1302
Size: 873 429
BsaHI Gr'CG_yC
Cuts at: 0 554 1187 1302
Size: 554 633 115
Fragments arranged by size:
633 554 115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 451 481 1302
Size: 451 30 821
Fragments arranged by size:
821 451 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1255 1302
Size: 1255 47
BseYI C'CCAG_C
Cuts at: 0 412 1091 1302
Size: 412 679 211
Fragments arranged by size:
679 412 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 1149 1302
Size: 55 1094 153
Fragments arranged by size:
1094 153 55
BsmBI CGTCTCn'nnnn_
Cuts at: 0 143 1302
Size: 143 1159
Bsp1286I G_dGCh'C
Cuts at: 0 107 223 469 1163 1302
Size: 107 116 246 694 139
Fragments arranged by size:
694 246 139 116 107
BspHI T'CATG_A
Cuts at: 0 503 1229 1302
Size: 503 726 73
Fragments arranged by size:
726 503 73
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 77 1302
Size: 77 1225
BsrBI CCG'CTC
Cuts at: 0 1115 1199 1302
Size: 1115 84 103
Fragments arranged by size:
1115 103 84
BsrDI GCAATG_nn'
Cuts at: 0 765 1139 1302
Size: 765 374 163
Fragments arranged by size:
765 374 163
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1267 1302
Size: 1267 35
BstEII G'GTnAC_C
Cuts at: 0 703 1047 1302
Size: 703 344 255
Fragments arranged by size:
703 344 255
BstXI CCAn_nnnn'nTGG
Cuts at: 0 618 651 700 1117 1302
Size: 618 33 49 417 185
Fragments arranged by size:
618 417 185 49 33
BstYI r'GATC_y
Cuts at: 0 405 1302
Size: 405 897
BtgI C'CryG_G
Cuts at: 0 53 119 1302
Size: 53 66 1183
Fragments arranged by size:
1183 66 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 384 1302
Size: 384 918
ClaI AT'CG_AT
Cuts at: 0 1057 1302
Size: 1057 245
DrdI GACnn_nn'nnGTC
Cuts at: 0 961 1302
Size: 961 341
EaeI y'GGCC_r
Cuts at: 0 2 77 347 488 686 1211 1302
Size: 2 75 270 141 198 525 91
Fragments arranged by size:
525 270 198 141 91 75 2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 208 673 691 700 1045 1302
Size: 24 184 465 18 9 345 257
Fragments arranged by size:
465 345 257 184 24 18 9
EcoO109I rG'GnC_Cy
Cuts at: 0 220 381 666 1302
Size: 220 161 285 636
Fragments arranged by size:
636 285 220 161
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1281 1302
Size: 1281 21
FspI TGC'GCA
Cuts at: 0 547 1302
Size: 547 755
FspAI rTGC'GCAy
Cuts at: 0 547 1302
Size: 547 755
HaeII r_GCGC'y
Cuts at: 0 557 1190 1302
Size: 557 633 112
Fragments arranged by size:
633 557 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 769 801 1302
Size: 292 32 445 32 501
Fragments arranged by size:
501 445 292 32 32
HincII GTy'rAC
Cuts at: 0 270 1032 1302
Size: 270 762 270
Fragments arranged by size:
762 270 270
KasI G'GCGC_C
Cuts at: 0 553 1186 1302
Size: 553 633 116
Fragments arranged by size:
633 553 116
MscI TGG'CCA
Cuts at: 0 4 490 1213 1302
Size: 4 486 723 89
Fragments arranged by size:
723 486 89 4
MslI CAynn'nnrTG
Cuts at: 0 616 733 1302
Size: 616 117 569
Fragments arranged by size:
616 569 117
MspA1I CmG'CkG
Cuts at: 0 100 370 1302
Size: 100 270 932
Fragments arranged by size:
932 270 100
NarI GG'CG_CC
Cuts at: 0 554 1187 1302
Size: 554 633 115
Fragments arranged by size:
633 554 115
PflMI CCAn_nnn'nTGG
Cuts at: 0 945 1302
Size: 945 357
PfoI T'CCnGG_A
Cuts at: 0 650 1302
Size: 650 652
PpuMI rG'GwC_Cy
Cuts at: 0 381 1302
Size: 381 921
PspOMI G'GGCC_C
Cuts at: 0 219 1302
Size: 219 1083
PvuII CAG'CTG
Cuts at: 0 100 370 1302
Size: 100 270 932
Fragments arranged by size:
932 270 100
SanDI GG'GwC_CC
Cuts at: 0 381 1302
Size: 381 921
ScaI AGT'ACT
Cuts at: 0 213 1302
Size: 213 1089
SfoI GGC'GCC
Cuts at: 0 555 1188 1302
Size: 555 633 114
Fragments arranged by size:
633 555 114
SmaI CCC'GGG
Cuts at: 0 25 1302
Size: 25 1277
SmlI C'TyrA_G
Cuts at: 0 574 1302
Size: 574 728
StuI AGG'CCT
Cuts at: 0 1006 1302
Size: 1006 296
StyI C'CwwG_G
Cuts at: 0 80 344 1302
Size: 80 264 958
Fragments arranged by size:
958 264 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 313 610 657 1066 1302
Size: 313 297 47 409 236
Fragments arranged by size:
409 313 297 236 47
TatI w'GTAC_w
Cuts at: 0 211 1302
Size: 211 1091
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 632 664 1302
Size: 632 32 638
Fragments arranged by size:
638 632 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 60 330 1302
Size: 60 270 972
Fragments arranged by size:
972 270 60
XmaI C'CCGG_G
Cuts at: 0 23 1302
Size: 23 1279
Enzymes that do cut:
AccI AcuI AhdI AleI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BclI BglI BglII
Bme1580I BmrI BpmI BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BstAPI BstEII BstXI BstYI BtgI BtgZI ClaI DrdI
EaeI Eco57MI EcoO109I FalI FspI FspAI HaeII Hin4I
HincII KasI MscI MslI MspA1I NarI PflMI PfoI
PpuMI PspOMI PvuII SanDI ScaI SfoI SmaI SmlI
StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BfrBI BlpI BmgBI BmtI BplI
Bpu10I BsaAI BsaBI BsaWI BsiEI BsiHKAI BsiWI BsmI
BspEI BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I
BtsI CspCI DraI DraIII EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FseI HindIII HpaI KpnI MfeI
MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SapI SbfI SexAI SfcI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmnI ZraI