Restriction map for
pFN21AE5508
(Linear) (Six-base) MAPSORT of: KIEE5508.seq Check: 4154 from: 1 to: 1311
KIEE5508.orf
With 182 enzymes: SgfI *
January 29, 2014 12:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 48 1085 1311
Size: 48 1037 226
Fragments arranged by size:
1037 226 48
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 1311
Size: 785 526
AlwNI CAG_nnn'CTG
Cuts at: 0 399 1311
Size: 399 912
ApaI G_GGCC'C
Cuts at: 0 67 992 1311
Size: 67 925 319
Fragments arranged by size:
925 319 67
ApoI r'AATT_y
Cuts at: 0 327 1311
Size: 327 984
AvaI C'yCGr_G
Cuts at: 0 332 1311
Size: 332 979
BanI G'GyrC_C
Cuts at: 0 823 1089 1170 1311
Size: 823 266 81 141
Fragments arranged by size:
823 266 141 81
BanII G_rGCy'C
Cuts at: 0 67 202 363 551 749 992 1311
Size: 67 135 161 188 198 243 319
Fragments arranged by size:
319 243 198 188 161 135 67
BbsI GAAGACnn'nnnn_
Cuts at: 0 1117 1311
Size: 1117 194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 424 458 1311
Size: 424 34 853
Fragments arranged by size:
853 424 34
BglII A'GATC_T
Cuts at: 0 911 1037 1311
Size: 911 126 274
Fragments arranged by size:
911 274 126
BlpI GC'TnA_GC
Cuts at: 0 643 1311
Size: 643 668
Bme1580I G_kGCm'C
Cuts at: 0 67 992 1175 1311
Size: 67 925 183 136
Fragments arranged by size:
925 183 136 67
BmgBI CAC'GTC
Cuts at: 0 945 1311
Size: 945 366
BmrI ACTGGGnnnn_n'
Cuts at: 0 409 645 1311
Size: 409 236 666
Fragments arranged by size:
666 409 236
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 367 1081 1311
Size: 367 714 230
Fragments arranged by size:
714 367 230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 763 1311
Size: 565 198 548
Fragments arranged by size:
565 548 198
Bpu10I CC'TnA_GC
Cuts at: 0 658 931 1311
Size: 658 273 380
Fragments arranged by size:
658 380 273
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 1291 1311
Size: 775 516 20
Fragments arranged by size:
775 516 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 472 1311
Size: 472 839
BsaAI yAC'GTr
Cuts at: 0 1218 1311
Size: 1218 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1207 1237 1311
Size: 1207 30 74
Fragments arranged by size:
1207 74 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1294 1311
Size: 1294 17
BseYI C'CCAG_C
Cuts at: 0 10 835 840 980 1311
Size: 10 825 5 140 331
Fragments arranged by size:
825 331 140 10 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1028 1311
Size: 1028 283
BsiHKAI G_wGCw'C
Cuts at: 0 551 749 1311
Size: 551 198 562
Fragments arranged by size:
562 551 198
BsmI GAATG_Cn'
Cuts at: 0 372 1311
Size: 372 939
Bsp1286I G_dGCh'C
Cuts at: 0 67 202 363 551 749 992 1175 1311
Size: 67 135 161 188 198 243 183 136
Fragments arranged by size:
243 198 188 183 161 136 135 67
BspHI T'CATG_A
Cuts at: 0 701 1311
Size: 701 610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 48 1085 1311
Size: 48 1037 226
Fragments arranged by size:
1037 226 48
BsrBI CCG'CTC
Cuts at: 0 84 1311
Size: 84 1227
BsrDI GCAATG_nn'
Cuts at: 0 828 1311
Size: 828 483
BsrGI T'GTAC_A
Cuts at: 0 1101 1311
Size: 1101 210
BstAPI GCAn_nnn'nTGC
Cuts at: 0 830 1311
Size: 830 481
BstXI CCAn_nnnn'nTGG
Cuts at: 0 992 1311
Size: 992 319
BstYI r'GATC_y
Cuts at: 0 564 683 911 1037 1311
Size: 564 119 228 126 274
Fragments arranged by size:
564 274 228 126 119
BtgI C'CryG_G
Cuts at: 0 263 568 1311
Size: 263 305 743
Fragments arranged by size:
743 305 263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1289 1311
Size: 1289 22
BtsI GCAGTG_nn'
Cuts at: 0 1124 1311
Size: 1124 187
EaeI y'GGCC_r
Cuts at: 0 192 1311
Size: 192 1119
EarI CTCTTCn'nnn_
Cuts at: 0 526 568 1028 1311
Size: 526 42 460 283
Fragments arranged by size:
526 460 283 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1276 1311
Size: 1276 35
EcoICRI GAG'CTC
Cuts at: 0 549 747 1311
Size: 549 198 564
Fragments arranged by size:
564 549 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 763 785 1311
Size: 565 198 22 526
Fragments arranged by size:
565 526 198 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 934 1081 1311
Size: 934 147 230
Fragments arranged by size:
934 230 147
EcoO109I rG'GnC_Cy
Cuts at: 0 64 336 852 988 989 1311
Size: 64 272 516 136 1 322
Fragments arranged by size:
516 322 272 136 64 1
EcoRI G'AATT_C
Cuts at: 0 327 1311
Size: 327 984
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 930 1311
Size: 930 381
HincII GTy'rAC
Cuts at: 0 380 1281 1311
Size: 380 901 30
Fragments arranged by size:
901 380 30
HindIII A'AGCT_T
Cuts at: 0 1162 1311
Size: 1162 149
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 413 539 1179 1311
Size: 141 272 126 640 132
Fragments arranged by size:
640 272 141 132 126
MscI TGG'CCA
Cuts at: 0 194 1311
Size: 194 1117
MspA1I CmG'CkG
Cuts at: 0 14 265 1311
Size: 14 251 1046
Fragments arranged by size:
1046 251 14
NcoI C'CATG_G
Cuts at: 0 568 1311
Size: 568 743
NruI TCG'CGA
Cuts at: 0 76 1311
Size: 76 1235
PasI CC'CwG_GG
Cuts at: 0 992 1311
Size: 992 319
PflMI CCAn_nnn'nTGG
Cuts at: 0 399 456 1311
Size: 399 57 855
Fragments arranged by size:
855 399 57
PfoI T'CCnGG_A
Cuts at: 0 424 740 1311
Size: 424 316 571
Fragments arranged by size:
571 424 316
PmlI CAC'GTG
Cuts at: 0 1218 1311
Size: 1218 93
PpuMI rG'GwC_Cy
Cuts at: 0 336 852 1311
Size: 336 516 459
Fragments arranged by size:
516 459 336
PspOMI G'GGCC_C
Cuts at: 0 63 988 1311
Size: 63 925 323
Fragments arranged by size:
925 323 63
PvuII CAG'CTG
Cuts at: 0 14 1311
Size: 14 1297
SacI G_AGCT'C
Cuts at: 0 551 749 1311
Size: 551 198 562
Fragments arranged by size:
562 551 198
SacII CC_GC'GG
Cuts at: 0 266 1311
Size: 266 1045
SapI GCTCTTCn'nnn_
Cuts at: 0 568 1311
Size: 568 743
ScaI AGT'ACT
Cuts at: 0 676 1311
Size: 676 635
SexAI A'CCwGG_T
Cuts at: 0 1134 1311
Size: 1134 177
SmaI CCC'GGG
Cuts at: 0 334 1311
Size: 334 977
SmlI C'TyrA_G
Cuts at: 0 790 1306 1311
Size: 790 516 5
Fragments arranged by size:
790 516 5
StuI AGG'CCT
Cuts at: 0 805 1311
Size: 805 506
StyI C'CwwG_G
Cuts at: 0 568 1311
Size: 568 743
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 841 1311
Size: 841 470
TatI w'GTAC_w
Cuts at: 0 674 1101 1311
Size: 674 427 210
Fragments arranged by size:
674 427 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1207 1239 1311
Size: 1207 32 72
Fragments arranged by size:
1207 72 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 396 817 1311
Size: 396 421 494
Fragments arranged by size:
494 421 396
XmaI C'CCGG_G
Cuts at: 0 332 1311
Size: 332 979
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApoI AvaI BanI BanII
BbsI BcgI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrGI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HincII HindIII MmeI MscI MspA1I NcoI NruI PasI
PflMI PfoI PmlI PpuMI PspOMI PvuII SacI SacII
SapI ScaI SexAI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BglI BmtI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI
BspEI BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EcoRV FseI
FspI FspAI HaeII Hin4I HpaI KasI KpnI MfeI
MluI MslI NaeI NarI NdeI NgoMIV NheI NotI
NsiI NspI PacI PciI PmeI PpiI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SalI SanDI SbfI
SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI