Restriction map for
pFN21AE5505
(Linear) (Six-base) MAPSORT of: KIEE5505.seq Check: 1480 from: 1 to: 1260
KIEE5505.orf
With 182 enzymes: SgfI *
January 29, 2014 12:03 ..
AccI GT'mk_AC
Cuts at: 0 710 1260
Size: 710 550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 1260
Size: 887 373
AlwNI CAG_nnn'CTG
Cuts at: 0 371 955 1260
Size: 371 584 305
Fragments arranged by size:
584 371 305
ApaI G_GGCC'C
Cuts at: 0 349 1260
Size: 349 911
BanII G_rGCy'C
Cuts at: 0 349 1260
Size: 349 911
BbsI GAAGACnn'nnnn_
Cuts at: 0 283 456 596 1146 1260
Size: 283 173 140 550 114
Fragments arranged by size:
550 283 173 140 114
BglII A'GATC_T
Cuts at: 0 640 1260
Size: 640 620
Bme1580I G_kGCm'C
Cuts at: 0 349 1260
Size: 349 911
BmrI ACTGGGnnnn_n'
Cuts at: 0 409 1260
Size: 409 851
BsaBI GATnn'nnATC
Cuts at: 0 1009 1260
Size: 1009 251
BseYI C'CCAG_C
Cuts at: 0 221 1260
Size: 221 1039
Bsp1286I G_dGCh'C
Cuts at: 0 349 1260
Size: 349 911
BspHI T'CATG_A
Cuts at: 0 173 1260
Size: 173 1087
BsrDI GCAATG_nn'
Cuts at: 0 377 1260
Size: 377 883
BstAPI GCAn_nnn'nTGC
Cuts at: 0 371 1260
Size: 371 889
BstYI r'GATC_y
Cuts at: 0 640 1260
Size: 640 620
BstZ17I GTA'TAC
Cuts at: 0 711 1260
Size: 711 549
BtgI C'CryG_G
Cuts at: 0 185 194 544 1260
Size: 185 9 350 716
Fragments arranged by size:
716 350 185 9
DraIII CAC_nnn'GTG
Cuts at: 0 992 1260
Size: 992 268
EaeI y'GGCC_r
Cuts at: 0 2 1260
Size: 2 1258
EarI CTCTTCn'nnn_
Cuts at: 0 623 906 1198 1260
Size: 623 283 292 62
Fragments arranged by size:
623 292 283 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 1260
Size: 887 373
EcoO109I rG'GnC_Cy
Cuts at: 0 345 346 1128 1260
Size: 345 1 782 132
Fragments arranged by size:
782 345 132 1
HincII GTy'rAC
Cuts at: 0 201 255 747 1176 1260
Size: 201 54 492 429 84
Fragments arranged by size:
492 429 201 84 54
HpaI GTT'AAC
Cuts at: 0 747 1176 1260
Size: 747 429 84
Fragments arranged by size:
747 429 84
MscI TGG'CCA
Cuts at: 0 4 1260
Size: 4 1256
MslI CAynn'nnrTG
Cuts at: 0 184 805 862 1260
Size: 184 621 57 398
Fragments arranged by size:
621 398 184 57
MspA1I CmG'CkG
Cuts at: 0 1109 1260
Size: 1109 151
NcoI C'CATG_G
Cuts at: 0 185 194 544 1260
Size: 185 9 350 716
Fragments arranged by size:
716 350 185 9
NdeI CA'TA_TG
Cuts at: 0 661 1260
Size: 661 599
PpuMI rG'GwC_Cy
Cuts at: 0 1128 1260
Size: 1128 132
PspOMI G'GGCC_C
Cuts at: 0 345 1260
Size: 345 915
PstI C_TGCA'G
Cuts at: 0 376 1260
Size: 376 884
SfcI C'TryA_G
Cuts at: 0 372 1260
Size: 372 888
StyI C'CwwG_G
Cuts at: 0 185 194 447 544 1033 1260
Size: 185 9 253 97 489 227
Fragments arranged by size:
489 253 227 185 97 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 440 1130 1260
Size: 440 690 130
Fragments arranged by size:
690 440 130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 192 1050 1260
Size: 192 858 210
Fragments arranged by size:
858 210 192
XmnI GAAnn'nnTTC
Cuts at: 0 1153 1260
Size: 1153 107
Enzymes that do cut:
AccI AcuI AlwNI ApaI BanII BbsI BglII Bme1580I
BmrI BsaBI BseYI Bsp1286I BspHI BsrDI BstAPI BstYI
BstZ17I BtgI DraIII EaeI EarI Eco57MI EcoO109I HincII
HpaI MscI MslI MspA1I NcoI NdeI PpuMI PspOMI
PstI SfcI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HindIII KasI KpnI
MfeI MluI MmeI NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmaI ZraI