Restriction map for
pFN21AE5504
(Linear) (Six-base) MAPSORT of: KIEE5504.seq Check: 9000 from: 1 to: 1260
KIEE5504.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
AatII G_ACGT'C
Cuts at: 0 1127 1260
Size: 1127 133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 695 1260
Size: 318 377 565
Fragments arranged by size:
565 377 318
AleI CACnn'nnGTG
Cuts at: 0 745 1260
Size: 745 515
AlwNI CAG_nnn'CTG
Cuts at: 0 1080 1260
Size: 1080 180
ApaI G_GGCC'C
Cuts at: 0 174 805 894 1106 1260
Size: 174 631 89 212 154
Fragments arranged by size:
631 212 174 154 89
AvaI C'yCGr_G
Cuts at: 0 1181 1255 1260
Size: 1181 74 5
Fragments arranged by size:
1181 74 5
BamHI G'GATC_C
Cuts at: 0 1112 1260
Size: 1112 148
BanII G_rGCy'C
Cuts at: 0 158 174 521 712 805 894 1106 1260
Size: 158 16 347 191 93 89 212 154
Fragments arranged by size:
347 212 191 158 154 93 89 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 522 639 1119 1143 1260
Size: 522 117 480 24 117
Fragments arranged by size:
522 480 117 117 24
BciVI GTATCCnnnnn_n'
Cuts at: 0 840 1260
Size: 840 420
BglI GCCn_nnn'nGGC
Cuts at: 0 1101 1260
Size: 1101 159
Bme1580I G_kGCm'C
Cuts at: 0 174 344 805 856 894 1106 1260
Size: 174 170 461 51 38 212 154
Fragments arranged by size:
461 212 174 170 154 51 38
BmgBI CAC'GTC
Cuts at: 0 857 1260
Size: 857 403
BmrI ACTGGGnnnn_n'
Cuts at: 0 437 1260
Size: 437 823
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1237 1260
Size: 1237 23
Bpu10I CC'TnA_GC
Cuts at: 0 448 1260
Size: 448 812
BsaBI GATnn'nnATC
Cuts at: 0 82 1260
Size: 82 1178
BsaHI Gr'CG_yC
Cuts at: 0 1049 1124 1260
Size: 1049 75 136
Fragments arranged by size:
1049 136 75
BsaWI w'CCGG_w
Cuts at: 0 1066 1260
Size: 1066 194
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 277 307 683 713 1260
Size: 277 30 376 30 547
Fragments arranged by size:
547 376 277 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 285 704 895 1260
Size: 285 419 191 365
Fragments arranged by size:
419 365 285 191
BseYI C'CCAG_C
Cuts at: 0 249 520 928 1260
Size: 249 271 408 332
Fragments arranged by size:
408 332 271 249
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 1260
Size: 247 1013
BsiEI CG_ry'CG
Cuts at: 0 1158 1260
Size: 1158 102
BsiHKAI G_wGCw'C
Cuts at: 0 158 952 1260
Size: 158 794 308
Fragments arranged by size:
794 308 158
Bsp1286I G_dGCh'C
Cuts at: 0 158 174 344 521 712 805 856 894
Size: 158 16 170 177 191 93 51 38
Cuts at: 894 952 1106 1260
Size: 58 154 154
Fragments arranged by size:
191 177 170 158 154 154 93 58
51 38 16
BsrBI CCG'CTC
Cuts at: 0 1216 1260
Size: 1216 44
BssHII G'CGCG_C
Cuts at: 0 1195 1197 1260
Size: 1195 2 63
Fragments arranged by size:
1195 63 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 27 1260
Size: 27 1233
BstXI CCAn_nnnn'nTGG
Cuts at: 0 397 550 1260
Size: 397 153 710
Fragments arranged by size:
710 397 153
BstYI r'GATC_y
Cuts at: 0 776 884 1112 1260
Size: 776 108 228 148
Fragments arranged by size:
776 228 148 108
BtgI C'CryG_G
Cuts at: 0 1158 1260
Size: 1158 102
EaeI y'GGCC_r
Cuts at: 0 1145 1260
Size: 1145 115
EarI CTCTTCn'nnn_
Cuts at: 0 95 671 1165 1260
Size: 95 576 494 95
Fragments arranged by size:
576 494 95 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1042 1082 1260
Size: 1042 40 178
Fragments arranged by size:
1042 178 40
EcoICRI GAG'CTC
Cuts at: 0 156 1260
Size: 156 1104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 695 1260
Size: 318 377 565
Fragments arranged by size:
565 377 318
EcoNI CCTnn'n_nnAGG
Cuts at: 0 451 686 1260
Size: 451 235 574
Fragments arranged by size:
574 451 235
EcoO109I rG'GnC_Cy
Cuts at: 0 116 170 171 309 669 871 891 1077
Size: 116 54 1 138 360 202 20 186
Cuts at: 1077 1102 1103 1260
Size: 25 1 157
Fragments arranged by size:
360 202 186 157 138 116 54 25
20 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 213 663 1260
Size: 213 450 597
Fragments arranged by size:
597 450 213
FspI TGC'GCA
Cuts at: 0 1241 1260
Size: 1241 19
HaeII r_GCGC'y
Cuts at: 0 1181 1260
Size: 1181 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 277 309 683 715 1260
Size: 277 32 374 32 545
Fragments arranged by size:
545 374 277 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 1260
Size: 126 1134
MslI CAynn'nnrTG
Cuts at: 0 548 745 1260
Size: 548 197 515
Fragments arranged by size:
548 515 197
MspA1I CmG'CkG
Cuts at: 0 15 1160 1260
Size: 15 1145 100
Fragments arranged by size:
1145 100 15
NspI r_CATG'y
Cuts at: 0 901 1260
Size: 901 359
PasI CC'CwG_GG
Cuts at: 0 181 805 1260
Size: 181 624 455
Fragments arranged by size:
624 455 181
PflMI CCAn_nnn'nTGG
Cuts at: 0 181 305 1080 1260
Size: 181 124 775 180
Fragments arranged by size:
775 181 180 124
PfoI T'CCnGG_A
Cuts at: 0 316 842 1260
Size: 316 526 418
Fragments arranged by size:
526 418 316
PpuMI rG'GwC_Cy
Cuts at: 0 116 309 669 871 1260
Size: 116 193 360 202 389
Fragments arranged by size:
389 360 202 193 116
PspOMI G'GGCC_C
Cuts at: 0 170 801 890 1102 1260
Size: 170 631 89 212 158
Fragments arranged by size:
631 212 170 158 89
PstI C_TGCA'G
Cuts at: 0 1101 1260
Size: 1101 159
SacI G_AGCT'C
Cuts at: 0 158 1260
Size: 158 1102
SacII CC_GC'GG
Cuts at: 0 1161 1260
Size: 1161 99
SanDI GG'GwC_CC
Cuts at: 0 309 669 1260
Size: 309 360 591
Fragments arranged by size:
591 360 309
SapI GCTCTTCn'nnn_
Cuts at: 0 671 1165 1260
Size: 671 494 95
Fragments arranged by size:
671 494 95
SbfI CC_TGCA'GG
Cuts at: 0 1101 1260
Size: 1101 159
SfcI C'TryA_G
Cuts at: 0 216 1097 1260
Size: 216 881 163
Fragments arranged by size:
881 216 163
SmaI CCC'GGG
Cuts at: 0 1183 1257 1260
Size: 1183 74 3
Fragments arranged by size:
1183 74 3
SphI G_CATG'C
Cuts at: 0 901 1260
Size: 901 359
StyI C'CwwG_G
Cuts at: 0 824 1260
Size: 824 436
TatI w'GTAC_w
Cuts at: 0 612 935 1260
Size: 612 323 325
Fragments arranged by size:
612 325 323
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 713 745 1104 1136 1260
Size: 713 32 359 32 124
Fragments arranged by size:
713 359 124 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 582 1260
Size: 582 678
XmaI C'CCGG_G
Cuts at: 0 1181 1255 1260
Size: 1181 74 5
Fragments arranged by size:
1181 74 5
XmnI GAAnn'nnTTC
Cuts at: 0 593 1260
Size: 593 667
ZraI GAC'GTC
Cuts at: 0 1125 1260
Size: 1125 135
Enzymes that do cut:
AatII AcuI AleI AlwNI ApaI AvaI BamHI BanII
BbsI BciVI BglI Bme1580I BmgBI BmrI BplI Bpu10I
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BsrBI BssHII BstAPI BstXI BstYI BtgI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I MmeI MslI MspA1I NspI PasI
PflMI PfoI PpuMI PspOMI PstI SacI SacII SanDI
SapI SbfI SfcI SmaI SphI StyI TatI TstI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbvCI BcgI BclI BfrBI
BglII BlpI BmtI BpmI BpuEI BsaI BsaAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EcoRI EcoRV FseI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SalI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XhoI