(Linear) (Six-base) MAPSORT of: KIEE5502.seq  Check: 943  from: 1  to: 1182



KIEE5502.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     818    1182 
   Size:        818     364
 
ApaLI G'TGCA_C

Cuts at:      0     152    1182 
   Size:        152    1030
 
ApoI r'AATT_y

Cuts at:      0     698    1023    1182 
   Size:        698     325     159

  Fragments arranged by size: 

                 698     325     159
 
AvaI C'yCGr_G

Cuts at:      0     740    1182 
   Size:        740     442
 
BclI T'GATC_A

Cuts at:      0    1155    1182 
   Size:       1155      27
 
BfrBI ATG'CAT

Cuts at:      0     537    1182 
   Size:        537     645
 
BglII A'GATC_T

Cuts at:      0     507    1182 
   Size:        507     675
 
Bme1580I G_kGCm'C

Cuts at:      0     156     424    1182 
   Size:        156     268     758

  Fragments arranged by size: 

                 758     268     156
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347    1182 
   Size:        347     835
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     476     662    1182 
   Size:        476     186     520

  Fragments arranged by size: 

                 520     476     186
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     442     823    1182 
   Size:        442     381     359

  Fragments arranged by size: 

                 442     381     359
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     260     987    1182 
   Size:        260     727     195

  Fragments arranged by size: 

                 727     260     195
 
BseYI C'CCAG_C

Cuts at:      0      70    1182 
   Size:         70    1112
 
BsiEI CG_ry'CG

Cuts at:      0      65    1182 
   Size:         65    1117
 
BsiHKAI G_wGCw'C

Cuts at:      0     156    1182 
   Size:        156    1026
 
Bsp1286I G_dGCh'C

Cuts at:      0     156     424    1182 
   Size:        156     268     758

  Fragments arranged by size: 

                 758     268     156
 
BspHI T'CATG_A

Cuts at:      0    1164    1182 
   Size:       1164      18
 
BssSI C'ACGA_G

Cuts at:      0    1049    1182 
   Size:       1049     133
 
BstYI r'GATC_y

Cuts at:      0     507    1182 
   Size:        507     675
 
BtsI GCAGTG_nn'

Cuts at:      0     392    1182 
   Size:        392     790
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     381     416    1182 
   Size:        381      35     766

  Fragments arranged by size: 

                 766     381      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     856    1182 
   Size:        856     326
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      61    1182 
   Size:         61    1121
 
EaeI y'GGCC_r

Cuts at:      0     297     389    1182 
   Size:        297      92     793

  Fragments arranged by size: 

                 793     297      92
 
EarI CTCTTCn'nnn_

Cuts at:      0     560     964    1182 
   Size:        560     404     218

  Fragments arranged by size: 

                 560     404     218
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347     818    1182 
   Size:        347     471     364

  Fragments arranged by size: 

                 471     364     347
 
EcoO109I rG'GnC_Cy

Cuts at:      0     125    1182 
   Size:        125    1057
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     644     676    1030    1062    1182 
   Size:        644      32     354      32     120

  Fragments arranged by size: 

                 644     354     120      32      32
 
MfeI C'AATT_G

Cuts at:      0      88     773    1182 
   Size:         88     685     409

  Fragments arranged by size: 

                 685     409      88
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      65      77    1109    1182 
   Size:         65      12    1032      73

  Fragments arranged by size: 

                1032      73      65      12
 
MscI TGG'CCA

Cuts at:      0     299     391    1182 
   Size:        299      92     791

  Fragments arranged by size: 

                 791     299      92
 
MslI CAynn'nnrTG

Cuts at:      0    1163    1182 
   Size:       1163      19
 
MspA1I CmG'CkG

Cuts at:      0      84     667     796    1182 
   Size:         84     583     129     386

  Fragments arranged by size: 

                 583     386     129      84
 
NdeI CA'TA_TG

Cuts at:      0     349    1182 
   Size:        349     833
 
NsiI A_TGCA'T

Cuts at:      0     539    1182 
   Size:        539     643
 
NspI r_CATG'y

Cuts at:      0     152    1117    1182 
   Size:        152     965      65

  Fragments arranged by size: 

                 965     152      65
 
PsiI TTA'TAA

Cuts at:      0     826    1182 
   Size:        826     356
 
PstI C_TGCA'G

Cuts at:      0     368    1182 
   Size:        368     814
 
SexAI A'CCwGG_T

Cuts at:      0     852    1182 
   Size:        852     330
 
SfcI C'TryA_G

Cuts at:      0     364    1182 
   Size:        364     818
 
SmlI C'TyrA_G

Cuts at:      0     491     677    1182 
   Size:        491     186     505

  Fragments arranged by size: 

                 505     491     186
 
StuI AGG'CCT

Cuts at:      0    1081    1182 
   Size:       1081     101
 
StyI C'CwwG_G

Cuts at:      0    1082    1182 
   Size:       1082     100
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      84    1182 
   Size:         84    1098
 
XbaI T'CTAG_A

Cuts at:      0     510    1182 
   Size:        510     672
 

 Enzymes that do cut:

     AcuI    ApaLI     ApoI     AvaI     BclI    BfrBI    BglII Bme1580I
     BpmI    BpuEI     BsaI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspHI    BssSI    BstYI     BtsI    CspCI   DraIII     DrdI     EaeI
     EarI  Eco57MI EcoO109I    Hin4I     MfeI     MmeI     MscI     MslI
   MspA1I     NdeI     NsiI     NspI     PsiI     PstI    SexAI     SfcI
     SmlI     StuI     StyI    TaqII     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BglI     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI     DraI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI