Restriction map for
pFN21AE5501
(Linear) (Six-base) MAPSORT of: KIEE5501.seq Check: 4991 from: 1 to: 1257
KIEE5501.orf
With 182 enzymes: SgfI *
January 29, 2014 12:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 149 1257
Size: 149 1108
AatII G_ACGT'C
Cuts at: 0 194 1257
Size: 194 1063
AccI GT'mk_AC
Cuts at: 0 869 1257
Size: 869 388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1257
Size: 499 758
AhdI GACnn_n'nnGTC
Cuts at: 0 279 1257
Size: 279 978
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 295 327 1257
Size: 295 32 930
Fragments arranged by size:
930 295 32
AlwNI CAG_nnn'CTG
Cuts at: 0 285 767 970 1257
Size: 285 482 203 287
Fragments arranged by size:
482 287 285 203
ApaI G_GGCC'C
Cuts at: 0 166 1257
Size: 166 1091
AvaI C'yCGr_G
Cuts at: 0 326 539 1257
Size: 326 213 718
Fragments arranged by size:
718 326 213
BanII G_rGCy'C
Cuts at: 0 166 1142 1257
Size: 166 976 115
Fragments arranged by size:
976 166 115
BciVI GTATCCnnnnn_n'
Cuts at: 0 950 1257
Size: 950 307
BglI GCCn_nnn'nGGC
Cuts at: 0 97 1257
Size: 97 1160
BglII A'GATC_T
Cuts at: 0 227 380 1257
Size: 227 153 877
Fragments arranged by size:
877 227 153
Bme1580I G_kGCm'C
Cuts at: 0 166 203 1257
Size: 166 37 1054
Fragments arranged by size:
1054 166 37
BmgBI CAC'GTC
Cuts at: 0 276 1208 1257
Size: 276 932 49
Fragments arranged by size:
932 276 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 777 1160 1257
Size: 777 383 97
Fragments arranged by size:
777 383 97
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 154 256 394 670 1257
Size: 145 9 102 138 276 587
Fragments arranged by size:
587 276 145 138 102 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 1257
Size: 575 682
BsaI GGTCTCn'nnnn_
Cuts at: 0 253 1257
Size: 253 1004
BsaAI yAC'GTr
Cuts at: 0 474 1257
Size: 474 783
BsaHI Gr'CG_yC
Cuts at: 0 191 462 1257
Size: 191 271 795
Fragments arranged by size:
795 271 191
BsaWI w'CCGG_w
Cuts at: 0 320 790 1257
Size: 320 470 467
Fragments arranged by size:
470 467 320
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 163 193 295 325 844 874 907 937
Size: 163 30 102 30 519 30 33 30
Cuts at: 937 1257
Size: 320
Fragments arranged by size:
519 320 163 102 33 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 310 391 922 1257
Size: 310 81 531 335
Fragments arranged by size:
531 335 310 81
BseYI C'CCAG_C
Cuts at: 0 208 926 1257
Size: 208 718 331
Fragments arranged by size:
718 331 208
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 1257
Size: 298 959
BsiHKAI G_wGCw'C
Cuts at: 0 77 140 1142 1238 1257
Size: 77 63 1002 96 19
Fragments arranged by size:
1002 96 77 63 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1198 1257
Size: 1198 59
Bsp1286I G_dGCh'C
Cuts at: 0 77 140 166 203 1142 1238 1257
Size: 77 63 26 37 939 96 19
Fragments arranged by size:
939 96 77 63 37 26 19
BspEI T'CCGG_A
Cuts at: 0 790 1257
Size: 790 467
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 149 407 1257
Size: 149 258 850
Fragments arranged by size:
850 258 149
BssHII G'CGCG_C
Cuts at: 0 331 1257
Size: 331 926
BstAPI GCAn_nnn'nTGC
Cuts at: 0 72 285 1257
Size: 72 213 972
Fragments arranged by size:
972 213 72
BstEII G'GTnAC_C
Cuts at: 0 549 1257
Size: 549 708
BstXI CCAn_nnnn'nTGG
Cuts at: 0 515 562 1257
Size: 515 47 695
Fragments arranged by size:
695 515 47
BstYI r'GATC_y
Cuts at: 0 227 380 434 1257
Size: 227 153 54 823
Fragments arranged by size:
823 227 153 54
BtgI C'CryG_G
Cuts at: 0 243 825 885 1257
Size: 243 582 60 372
Fragments arranged by size:
582 372 243 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 424 1257
Size: 424 833
EcoICRI GAG'CTC
Cuts at: 0 1140 1257
Size: 1140 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 154 256 394 499 670 1257
Size: 145 9 102 138 105 171 587
Fragments arranged by size:
587 171 145 138 105 102 9
EcoO109I rG'GnC_Cy
Cuts at: 0 129 162 163 1257
Size: 129 33 1 1094
Fragments arranged by size:
1094 129 33 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 72 1143 1257
Size: 72 1071 114
Fragments arranged by size:
1071 114 72
HaeII r_GCGC'y
Cuts at: 0 1158 1219 1257
Size: 1158 61 38
Fragments arranged by size:
1158 61 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 844 876 1257
Size: 265 32 547 32 381
Fragments arranged by size:
547 381 265 32 32
MslI CAynn'nnrTG
Cuts at: 0 472 1257
Size: 472 785
MspA1I CmG'CkG
Cuts at: 0 147 827 1257
Size: 147 680 430
Fragments arranged by size:
680 430 147
NcoI C'CATG_G
Cuts at: 0 885 1257
Size: 885 372
NspI r_CATG'y
Cuts at: 0 705 1257
Size: 705 552
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 1257
Size: 372 885
PfoI T'CCnGG_A
Cuts at: 0 392 647 1142 1257
Size: 392 255 495 115
Fragments arranged by size:
495 392 255 115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 1257
Size: 295 32 930
Fragments arranged by size:
930 295 32
PpuMI rG'GwC_Cy
Cuts at: 0 129 1257
Size: 129 1128
PspOMI G'GGCC_C
Cuts at: 0 162 1257
Size: 162 1095
PstI C_TGCA'G
Cuts at: 0 146 1257
Size: 146 1111
PvuII CAG'CTG
Cuts at: 0 147 1257
Size: 147 1110
SacI G_AGCT'C
Cuts at: 0 1142 1257
Size: 1142 115
SacII CC_GC'GG
Cuts at: 0 828 1257
Size: 828 429
ScaI AGT'ACT
Cuts at: 0 1123 1257
Size: 1123 134
SfcI C'TryA_G
Cuts at: 0 86 142 1104 1257
Size: 86 56 962 153
Fragments arranged by size:
962 153 86 56
SmaI CCC'GGG
Cuts at: 0 328 541 1257
Size: 328 213 716
Fragments arranged by size:
716 328 213
SmlI C'TyrA_G
Cuts at: 0 590 1257
Size: 590 667
StuI AGG'CCT
Cuts at: 0 901 1257
Size: 901 356
StyI C'CwwG_G
Cuts at: 0 635 885 998 1199 1257
Size: 635 250 113 201 58
Fragments arranged by size:
635 250 201 113 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 568 707 1257
Size: 568 139 550
Fragments arranged by size:
568 550 139
TatI w'GTAC_w
Cuts at: 0 1121 1257
Size: 1121 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 163 195 907 939 1257
Size: 163 32 712 32 318
Fragments arranged by size:
712 318 163 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 717 1257
Size: 717 540
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 846 1257
Size: 846 411
XmaI C'CCGG_G
Cuts at: 0 326 539 1257
Size: 326 213 718
Fragments arranged by size:
718 326 213
ZraI GAC'GTC
Cuts at: 0 192 1257
Size: 192 1065
Enzymes that do cut:
AarI AatII AccI AcuI AhdI AloI AlwNI ApaI
AvaI BanII BciVI BglI BglII Bme1580I BmgBI BmrI
BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BssHII
BstAPI BstEII BstXI BstYI BtgI EciI EcoICRI Eco57MI
EcoO109I FalI HaeII Hin4I MslI MspA1I NcoI NspI
PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI
SacII ScaI SfcI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BbvCI BcgI BclI BfrBI BlpI
BmtI BplI Bpu10I BsaBI BsiEI BsiWI BsmI BspHI
BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmnI