Restriction map for
pFN21AE5495
(Linear) (Six-base) MAPSORT of: KIEE5495.seq Check: 7389 from: 1 to: 1044
KIEE5495.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 823 1044
Size: 823 221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 132 1018 1044
Size: 48 84 886 26
Fragments arranged by size:
886 84 48 26
AleI CACnn'nnGTG
Cuts at: 0 943 1044
Size: 943 101
AlwNI CAG_nnn'CTG
Cuts at: 0 379 1044
Size: 379 665
ApoI r'AATT_y
Cuts at: 0 427 1044
Size: 427 617
AvaI C'yCGr_G
Cuts at: 0 608 1044
Size: 608 436
BamHI G'GATC_C
Cuts at: 0 604 1044
Size: 604 440
BanII G_rGCy'C
Cuts at: 0 553 996 1044
Size: 553 443 48
Fragments arranged by size:
553 443 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 915 1044
Size: 915 129
BclI T'GATC_A
Cuts at: 0 731 1044
Size: 731 313
BglI GCCn_nnn'nGGC
Cuts at: 0 448 1044
Size: 448 596
BglII A'GATC_T
Cuts at: 0 368 1044
Size: 368 676
Bme1580I G_kGCm'C
Cuts at: 0 332 1044
Size: 332 712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 1044
Size: 926 118
BsaAI yAC'GTr
Cuts at: 0 408 465 1044
Size: 408 57 579
Fragments arranged by size:
579 408 57
BsaHI Gr'CG_yC
Cuts at: 0 251 1044
Size: 251 793
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 558 588 743 773 1044
Size: 558 30 155 30 271
Fragments arranged by size:
558 271 155 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 706 751 810 1044
Size: 706 45 59 234
Fragments arranged by size:
706 234 59 45
BseYI C'CCAG_C
Cuts at: 0 880 1044
Size: 880 164
Bsp1286I G_dGCh'C
Cuts at: 0 332 553 996 1044
Size: 332 221 443 48
Fragments arranged by size:
443 332 221 48
BspHI T'CATG_A
Cuts at: 0 500 1044
Size: 500 544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 409 823 1029 1044
Size: 409 414 206 15
Fragments arranged by size:
414 409 206 15
BstYI r'GATC_y
Cuts at: 0 368 604 1044
Size: 368 236 440
Fragments arranged by size:
440 368 236
BtgI C'CryG_G
Cuts at: 0 446 600 771 1044
Size: 446 154 171 273
Fragments arranged by size:
446 273 171 154
EaeI y'GGCC_r
Cuts at: 0 20 86 104 467 1044
Size: 20 66 18 363 577
Fragments arranged by size:
577 363 66 20 18
EarI CTCTTCn'nnn_
Cuts at: 0 67 151 526 850 1044
Size: 67 84 375 324 194
Fragments arranged by size:
375 324 194 84 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 132 926 1018 1044
Size: 48 84 794 92 26
Fragments arranged by size:
794 92 84 48 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 346 1044
Size: 346 698
HincII GTy'rAC
Cuts at: 0 1010 1044
Size: 1010 34
HpaI GTT'AAC
Cuts at: 0 1010 1044
Size: 1010 34
MfeI C'AATT_G
Cuts at: 0 289 1044
Size: 289 755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 104 1044
Size: 20 84 940
Fragments arranged by size:
940 84 20
MscI TGG'CCA
Cuts at: 0 22 88 106 469 1044
Size: 22 66 18 363 575
Fragments arranged by size:
575 363 66 22 18
MslI CAynn'nnrTG
Cuts at: 0 16 52 100 190 268 304 358 583
Size: 16 36 48 90 78 36 54 225
Cuts at: 583 724 871 943 1044
Size: 141 147 72 101
Fragments arranged by size:
225 147 141 101 90 78 72 54
48 36 36 16
NcoI C'CATG_G
Cuts at: 0 446 1044
Size: 446 598
NspI r_CATG'y
Cuts at: 0 876 1044
Size: 876 168
PflMI CCAn_nnn'nTGG
Cuts at: 0 354 1044
Size: 354 690
PfoI T'CCnGG_A
Cuts at: 0 341 1044
Size: 341 703
PshAI GACnn'nnGTC
Cuts at: 0 835 1044
Size: 835 209
SexAI A'CCwGG_T
Cuts at: 0 347 1044
Size: 347 697
SfcI C'TryA_G
Cuts at: 0 963 1044
Size: 963 81
SmaI CCC'GGG
Cuts at: 0 610 1044
Size: 610 434
StuI AGG'CCT
Cuts at: 0 805 1044
Size: 805 239
StyI C'CwwG_G
Cuts at: 0 446 1044
Size: 446 598
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 346 1044
Size: 346 698
TatI w'GTAC_w
Cuts at: 0 272 692 1044
Size: 272 420 352
Fragments arranged by size:
420 352 272
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 558 590 1044
Size: 558 32 454
Fragments arranged by size:
558 454 32
Tth111I GACn'n_nGTC
Cuts at: 0 349 1044
Size: 349 695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 81 339 1044
Size: 81 258 705
Fragments arranged by size:
705 258 81
XmaI C'CCGG_G
Cuts at: 0 608 1044
Size: 608 436
Enzymes that do cut:
AarI AcuI AleI AlwNI ApoI AvaI BamHI BanII
BbsI BclI BglI BglII Bme1580I BpmI BsaAI BsaHI
BsaXI BseRI BseYI Bsp1286I BspHI BspMI BstYI BtgI
EaeI EarI Eco57MI EcoNI HincII HpaI MfeI MmeI
MscI MslI NcoI NspI PflMI PfoI PshAI SexAI
SfcI SmaI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbvCI BcgI BciVI BfrBI
BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HindIII KasI KpnI MluI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI
XmnI ZraI