(Linear) (Six-base) MAPSORT of: KIEE5492.seq  Check: 2758  from: 1  to: 1035



KIEE5492.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     869    1035 
   Size:        869     166
 
AlwNI CAG_nnn'CTG

Cuts at:      0      15    1035 
   Size:         15    1020
 
ApoI r'AATT_y

Cuts at:      0      94    1035 
   Size:         94     941
 
AvrII C'CTAG_G

Cuts at:      0     865    1035 
   Size:        865     170
 
BamHI G'GATC_C

Cuts at:      0     120    1035 
   Size:        120     915
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     877    1035 
   Size:        877     158
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      28    1035 
   Size:         28    1007
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     977    1035 
   Size:        977      58
 
BglI GCCn_nnn'nGGC

Cuts at:      0     485    1035 
   Size:        485     550
 
BglII A'GATC_T

Cuts at:      0     362    1035 
   Size:        362     673
 
Bpu10I CC'TnA_GC

Cuts at:      0     608    1035 
   Size:        608     427
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     984    1035 
   Size:        984      51
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     794    1035 
   Size:        794     241
 
BsaAI yAC'GTr

Cuts at:      0     341    1035 
   Size:        341     694
 
BsaWI w'CCGG_w

Cuts at:      0     123    1035 
   Size:        123     912
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     552    1035 
   Size:        552     483
 
BsmI GAATG_Cn'

Cuts at:      0     531     749    1035 
   Size:        531     218     286

  Fragments arranged by size: 

                 531     286     218
 
BspEI T'CCGG_A

Cuts at:      0     123    1035 
   Size:        123     912
 
BspHI T'CATG_A

Cuts at:      0     467    1035 
   Size:        467     568
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     197     814    1035 
   Size:        197     617     221

  Fragments arranged by size: 

                 617     221     197
 
BsrBI CCG'CTC

Cuts at:      0     588    1035 
   Size:        588     447
 
BsrDI GCAATG_nn'

Cuts at:      0     419    1035 
   Size:        419     616
 
BsrGI T'GTAC_A

Cuts at:      0     836    1035 
   Size:        836     199
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      15    1035 
   Size:         15    1020
 
BstEII G'GTnAC_C

Cuts at:      0     171    1035 
   Size:        171     864
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     168    1035 
   Size:        168     867
 
BstYI r'GATC_y

Cuts at:      0     120     362    1035 
   Size:        120     242     673

  Fragments arranged by size: 

                 673     242     120
 
BtsI GCAGTG_nn'

Cuts at:      0     884    1035 
   Size:        884     151
 
EaeI y'GGCC_r

Cuts at:      0     434    1035 
   Size:        434     601
 
EarI CTCTTCn'nnn_

Cuts at:      0     595     998    1035 
   Size:        595     403      37

  Fragments arranged by size: 

                 595     403      37
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     869    1035 
   Size:        869     166
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      38      70     105     137     446     478     975    1007 
   Size:         38      32      35      32     309      32     497      32

Cuts at:   1007    1035 
   Size:         28

  Fragments arranged by size: 

                 497     309      38      35      32      32      32      32
                  28
 
HincII GTy'rAC

Cuts at:      0     512    1035 
   Size:        512     523
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     642    1035 
   Size:        642     393
 
MscI TGG'CCA

Cuts at:      0     436    1035 
   Size:        436     599
 
MslI CAynn'nnrTG

Cuts at:      0     235    1035 
   Size:        235     800
 
MspA1I CmG'CkG

Cuts at:      0      12    1035 
   Size:         12    1023
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     886     918    1035 
   Size:        886      32     117

  Fragments arranged by size: 

                 886     117      32
 
PstI C_TGCA'G

Cuts at:      0     134     879    1035 
   Size:        134     745     156

  Fragments arranged by size: 

                 745     156     134
 
PvuII CAG'CTG

Cuts at:      0      12    1035 
   Size:         12    1023
 
SapI GCTCTTCn'nnn_

Cuts at:      0     595    1035 
   Size:        595     440
 
SfcI C'TryA_G

Cuts at:      0     130     875     930    1035 
   Size:        130     745      55     105

  Fragments arranged by size: 

                 745     130     105      55
 
SmlI C'TyrA_G

Cuts at:      0     963    1035 
   Size:        963      72
 
SpeI A'CTAG_T

Cuts at:      0     514    1035 
   Size:        514     521
 
StyI C'CwwG_G

Cuts at:      0     865    1035 
   Size:        865     170
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     373    1035 
   Size:        373     662
 
TatI w'GTAC_w

Cuts at:      0     164     239     836    1035 
   Size:        164      75     597     199

  Fragments arranged by size: 

                 597     199     164      75
 
XbaI T'CTAG_A

Cuts at:      0    1029    1035 
   Size:       1029       6
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    AvrII    BamHI     BbsI     BcgI    BciVI
     BglI    BglII   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI     BsgI
     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BstAPI
   BstEII    BstXI    BstYI     BtsI     EaeI     EarI  Eco57MI    Hin4I
   HincII     MmeI     MscI     MslI   MspA1I     PsrI     PstI    PvuII
     SapI     SfcI     SmlI     SpeI     StyI    TaqII     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI     BaeI     BanI    BanII     BbeI    BbvCI
     BclI    BfrBI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI    BsaBI    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BsrFI   BssHII    BssSI    BstBI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XcmI
     XhoI     XmaI     XmnI     ZraI