(Linear) (Six-base) MAPSORT of: KIEE5491.seq  Check: 1918  from: 1  to: 1032



KIEE5491.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     434     970    1032 
   Size:         62     372     536      62

  Fragments arranged by size: 

                 536     372      62      62
 
AflII C'TTAA_G

Cuts at:      0     757    1032 
   Size:        757     275
 
AhdI GACnn_n'nnGTC

Cuts at:      0     598    1032 
   Size:        598     434
 
AlwNI CAG_nnn'CTG

Cuts at:      0     246    1032 
   Size:        246     786
 
ApoI r'AATT_y

Cuts at:      0      25     115    1032 
   Size:         25      90     917

  Fragments arranged by size: 

                 917      90      25
 
BanII G_rGCy'C

Cuts at:      0     134    1032 
   Size:        134     898
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     638    1032 
   Size:        638     394
 
BclI T'GATC_A

Cuts at:      0     482    1032 
   Size:        482     550
 
BlpI GC'TnA_GC

Cuts at:      0    1005    1032 
   Size:       1005      27
 
BmgBI CAC'GTC

Cuts at:      0     813    1032 
   Size:        813     219
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     677    1032 
   Size:        677     355
 
BsaBI GATnn'nnATC

Cuts at:      0     626    1032 
   Size:        626     406
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     707    1032 
   Size:        707     325
 
BseYI C'CCAG_C

Cuts at:      0    1009    1032 
   Size:       1009      23
 
BsmI GAATG_Cn'

Cuts at:      0     960    1032 
   Size:        960      72
 
Bsp1286I G_dGCh'C

Cuts at:      0     134    1032 
   Size:        134     898
 
BspHI T'CATG_A

Cuts at:      0     474    1032 
   Size:        474     558
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     218    1032 
   Size:        218     814
 
Bsu36I CC'TnA_GG

Cuts at:      0     146    1032 
   Size:        146     886
 
ClaI AT'CG_AT

Cuts at:      0     447    1032 
   Size:        447     585
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      65    1032 
   Size:         65     967
 
EaeI y'GGCC_r

Cuts at:      0     233     455    1032 
   Size:        233     222     577

  Fragments arranged by size: 

                 577     233     222
 
EarI CTCTTCn'nnn_

Cuts at:      0     476    1032 
   Size:        476     556
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     434     970    1032 
   Size:         62     372     536      62

  Fragments arranged by size: 

                 536     372      62      62
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     439    1032 
   Size:        439     593
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       3      35     325     357     584     616    1032 
   Size:          3      32     290      32     227      32     416

  Fragments arranged by size: 

                 416     290     227      32      32      32       3
 
HincII GTy'rAC

Cuts at:      0     333    1032 
   Size:        333     699
 
HindIII A'AGCT_T

Cuts at:      0     788    1002    1032 
   Size:        788     214      30

  Fragments arranged by size: 

                 788     214      30
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      81      94    1032 
   Size:         81      13     938

  Fragments arranged by size: 

                 938      81      13
 
MscI TGG'CCA

Cuts at:      0     235     457    1032 
   Size:        235     222     575

  Fragments arranged by size: 

                 575     235     222
 
PasI CC'CwG_GG

Cuts at:      0     246    1032 
   Size:        246     786
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     246    1032 
   Size:        246     786
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     491     523    1032 
   Size:        491      32     509

  Fragments arranged by size: 

                 509     491      32
 
PsiI TTA'TAA

Cuts at:      0     916    1032 
   Size:        916     116
 
SfcI C'TryA_G

Cuts at:      0     899    1032 
   Size:        899     133
 
SmlI C'TyrA_G

Cuts at:      0     757    1032 
   Size:        757     275
 
TatI w'GTAC_w

Cuts at:      0     260    1032 
   Size:        260     772
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     243    1032 
   Size:        243     789
 
XmnI GAAnn'nnTTC

Cuts at:      0     419    1032 
   Size:        419     613
 

 Enzymes that do cut:

     AcuI    AflII     AhdI    AlwNI     ApoI    BanII    BciVI     BclI
     BlpI    BmgBI     BmrI    BsaBI    BseRI    BseYI     BsmI Bsp1286I
    BspHI    BspMI   Bsu36I     ClaI     DrdI     EaeI     EarI  Eco57MI
     FalI    Hin4I   HincII  HindIII     MmeI     MscI     PasI    PflMI
     PpiI     PsiI     SfcI     SmlI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BfrBI     BglI    BglII Bme1580I     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PfoI     PmeI     PmlI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XhoI     XmaI     ZraI