(Linear) (Six-base) MAPSORT of: KIEE5488.seq  Check: 8625  from: 1  to: 1026



KIEE5488.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     769    1026 
   Size:        769     257
 
AleI CACnn'nnGTG

Cuts at:      0     776    1026 
   Size:        776     250
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     188     220    1026 
   Size:        188      32     806

  Fragments arranged by size: 

                 806     188      32
 
AvaI C'yCGr_G

Cuts at:      0     308    1026 
   Size:        308     718
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      11     285    1026 
   Size:         11     274     741

  Fragments arranged by size: 

                 741     274      11
 
BbvCI CC'TCA_GC

Cuts at:      0     718    1026 
   Size:        718     308
 
BclI T'GATC_A

Cuts at:      0     701    1026 
   Size:        701     325
 
BlpI GC'TnA_GC

Cuts at:      0      62    1026 
   Size:         62     964
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     105    1026 
   Size:        105     921
 
Bpu10I CC'TnA_GC

Cuts at:      0     253     718    1026 
   Size:        253     465     308

  Fragments arranged by size: 

                 465     308     253
 
BsaBI GATnn'nnATC

Cuts at:      0     124    1026 
   Size:        124     902
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     106     136     188     218    1026 
   Size:        106      30      52      30     808

  Fragments arranged by size: 

                 808     106      52      30      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     591     747    1026 
   Size:        591     156     279

  Fragments arranged by size: 

                 591     279     156
 
BsiHKAI G_wGCw'C

Cuts at:      0     902    1026 
   Size:        902     124
 
BsmI GAATG_Cn'

Cuts at:      0      89    1026 
   Size:         89     937
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     162    1026 
   Size:        162     864
 
Bsp1286I G_dGCh'C

Cuts at:      0     902    1026 
   Size:        902     124
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     769    1026 
   Size:        769     257
 
BsrDI GCAATG_nn'

Cuts at:      0      56     332     722    1026 
   Size:         56     276     390     304

  Fragments arranged by size: 

                 390     304     276      56
 
BsrGI T'GTAC_A

Cuts at:      0     268    1026 
   Size:        268     758
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     832    1026 
   Size:        832     194
 
BstYI r'GATC_y

Cuts at:      0     119    1026 
   Size:        119     907
 
BtgI C'CryG_G

Cuts at:      0     392    1026 
   Size:        392     634
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     811    1026 
   Size:        811     215
 
EaeI y'GGCC_r

Cuts at:      0     389     413     834     869    1026 
   Size:        389      24     421      35     157

  Fragments arranged by size: 

                 421     389     157      35      24
 
EarI CTCTTCn'nnn_

Cuts at:      0     731    1026 
   Size:        731     295
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     759    1026 
   Size:        759     267
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     701     733    1026 
   Size:        701      32     293

  Fragments arranged by size: 

                 701     293      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     621    1026 
   Size:        621     405
 
MscI TGG'CCA

Cuts at:      0     391     415     836     871    1026 
   Size:        391      24     421      35     155

  Fragments arranged by size: 

                 421     391     155      35      24
 
MslI CAynn'nnrTG

Cuts at:      0     776     830     865    1026 
   Size:        776      54      35     161

  Fragments arranged by size: 

                 776     161      54      35
 
NcoI C'CATG_G

Cuts at:      0     392    1026 
   Size:        392     634
 
NspI r_CATG'y

Cuts at:      0     486    1026 
   Size:        486     540
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     188     220    1026 
   Size:        188      32     806

  Fragments arranged by size: 

                 806     188      32
 
PstI C_TGCA'G

Cuts at:      0     550    1026 
   Size:        550     476
 
ScaI AGT'ACT

Cuts at:      0     115    1026 
   Size:        115     911
 
SfcI C'TryA_G

Cuts at:      0     546     659    1026 
   Size:        546     113     367

  Fragments arranged by size: 

                 546     367     113
 
StuI AGG'CCT

Cuts at:      0      98    1026 
   Size:         98     928
 
StyI C'CwwG_G

Cuts at:      0     285     392     633    1026 
   Size:        285     107     241     393

  Fragments arranged by size: 

                 393     285     241     107
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      30     124    1026 
   Size:         30      94     902

  Fragments arranged by size: 

                 902      94      30
 
TatI w'GTAC_w

Cuts at:      0     113     218     268    1026 
   Size:        113     105      50     758

  Fragments arranged by size: 

                 758     113     105      50
 
XmnI GAAnn'nnTTC

Cuts at:      0     751    1026 
   Size:        751     275
 

 Enzymes that do cut:

     AarI     AleI     AloI     AvaI     BbsI    BbvCI     BclI     BlpI
     BmrI   Bpu10I    BsaBI    BsaXI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspMI    BsrDI    BsrGI    BstXI    BstYI     BtgI    BtgZI
     EaeI     EarI     EciI    Hin4I     MmeI     MscI     MslI     NcoI
     NspI     PpiI     PstI     ScaI     SfcI     StuI     StyI    TaqII
     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseRI
    BseYI    BsiEI    BsiWI    BspEI    BspHI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI