(Linear) (Six-base) MAPSORT of: KIEE5487.seq  Check: 1823  from: 1  to: 1020



KIEE5487.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     131     787     875    1020 
   Size:        118      13     656      88     145

  Fragments arranged by size: 

                 656     145     118      88      13
 
AflIII A'CryG_T

Cuts at:      0     808    1020 
   Size:        808     212
 
AhdI GACnn_n'nnGTC

Cuts at:      0      63    1020 
   Size:         63     957
 
AleI CACnn'nnGTG

Cuts at:      0     928    1020 
   Size:        928      92
 
BamHI G'GATC_C

Cuts at:      0     598    1020 
   Size:        598     422
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     156    1020 
   Size:        156     864
 
BclI T'GATC_A

Cuts at:      0     450    1020 
   Size:        450     570
 
BfrBI ATG'CAT

Cuts at:      0     774    1020 
   Size:        774     246
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     848    1020 
   Size:        848     172
 
Bpu10I CC'TnA_GC

Cuts at:      0     782    1020 
   Size:        782     238
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     864    1020 
   Size:        864     156
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     626    1020 
   Size:        626     394
 
BsmI GAATG_Cn'

Cuts at:      0     774    1020 
   Size:        774     246
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      44    1020 
   Size:         44     976
 
BsrDI GCAATG_nn'

Cuts at:      0     446    1020 
   Size:        446     574
 
BstYI r'GATC_y

Cuts at:      0     598    1020 
   Size:        598     422
 
BtsI GCAGTG_nn'

Cuts at:      0     524     995    1020 
   Size:        524     471      25

  Fragments arranged by size: 

                 524     471      25
 
EarI CTCTTCn'nnn_

Cuts at:      0     691    1020 
   Size:        691     329
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     131     787     848     875    1020 
   Size:        118      13     656      61      27     145

  Fragments arranged by size: 

                 656     145     118      61      27      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      49      81     825     857    1020 
   Size:         49      32     744      32     163

  Fragments arranged by size: 

                 744     163      49      32      32
 
MfeI C'AATT_G

Cuts at:      0     926    1020 
   Size:        926      94
 
MslI CAynn'nnrTG

Cuts at:      0     907     928    1020 
   Size:        907      21      92

  Fragments arranged by size: 

                 907      92      21
 
NsiI A_TGCA'T

Cuts at:      0     776    1020 
   Size:        776     244
 
NspI r_CATG'y

Cuts at:      0     498     556     619     812    1020 
   Size:        498      58      63     193     208

  Fragments arranged by size: 

                 498     208     193      63      58
 
PciI A'CATG_T

Cuts at:      0     808    1020 
   Size:        808     212
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     861     893    1020 
   Size:        861      32     127

  Fragments arranged by size: 

                 861     127      32
 
PshAI GACnn'nnGTC

Cuts at:      0     112    1020 
   Size:        112     908
 
PsiI TTA'TAA

Cuts at:      0      32    1020 
   Size:         32     988
 
SfcI C'TryA_G

Cuts at:      0     311     416     957    1020 
   Size:        311     105     541      63

  Fragments arranged by size: 

                 541     311     105      63
 
SspI AAT'ATT

Cuts at:      0     917    1020 
   Size:        917     103
 
StuI AGG'CCT

Cuts at:      0     799    1020 
   Size:        799     221
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     516    1020 
   Size:        516     504
 
XmnI GAAnn'nnTTC

Cuts at:      0     425    1020 
   Size:        425     595
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AleI    BamHI     BbsI     BclI    BfrBI
     BpmI   Bpu10I     BsaI    BseRI     BsmI    BspMI    BsrDI    BstYI
     BtsI     EarI  Eco57MI    Hin4I     MfeI     MslI     NsiI     NspI
     PciI     PpiI    PshAI     PsiI     SfcI     SspI     StuI    TaqII
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI    PflMI     PfoI     PmeI     PmlI    PpuMI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI