Restriction map for
pFN21AE5482
(Linear) (Six-base) MAPSORT of: KIEE5482.seq Check: 3370 from: 1 to: 1005
KIEE5482.orf
With 182 enzymes: SgfI *
December 5, 2014 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 802 1005
Size: 802 203
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 610 1005
Size: 108 502 395
Fragments arranged by size:
502 395 108
AflIII A'CryG_T
Cuts at: 0 487 728 1005
Size: 487 241 277
Fragments arranged by size:
487 277 241
AleI CACnn'nnGTG
Cuts at: 0 492 1005
Size: 492 513
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 478 1005
Size: 478 527
AlwNI CAG_nnn'CTG
Cuts at: 0 633 1005
Size: 633 372
ApoI r'AATT_y
Cuts at: 0 976 1005
Size: 976 29
AvaI C'yCGr_G
Cuts at: 0 587 1005
Size: 587 418
AvrII C'CTAG_G
Cuts at: 0 261 1005
Size: 261 744
BamHI G'GATC_C
Cuts at: 0 7 1005
Size: 7 998
BanII G_rGCy'C
Cuts at: 0 143 1005
Size: 143 862
BclI T'GATC_A
Cuts at: 0 320 1005
Size: 320 685
BmgBI CAC'GTC
Cuts at: 0 490 1005
Size: 490 515
Bpu10I CC'TnA_GC
Cuts at: 0 872 1005
Size: 872 133
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 382 1005
Size: 142 240 623
Fragments arranged by size:
623 240 142
BseYI C'CCAG_C
Cuts at: 0 371 1005
Size: 371 634
BsiHKAI G_wGCw'C
Cuts at: 0 143 1005
Size: 143 862
Bsp1286I G_dGCh'C
Cuts at: 0 143 1005
Size: 143 862
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 802 1005
Size: 802 203
BstYI r'GATC_y
Cuts at: 0 7 1005
Size: 7 998
BtgI C'CryG_G
Cuts at: 0 65 425 1005
Size: 65 360 580
Fragments arranged by size:
580 360 65
EaeI y'GGCC_r
Cuts at: 0 422 1005
Size: 422 583
EarI CTCTTCn'nnn_
Cuts at: 0 160 1005
Size: 160 845
EcoICRI GAG'CTC
Cuts at: 0 141 1005
Size: 141 864
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 610 1005
Size: 108 502 395
Fragments arranged by size:
502 395 108
EcoRV GAT'ATC
Cuts at: 0 668 1005
Size: 668 337
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 801 1005
Size: 801 204
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 216 248 924 956 1005
Size: 216 32 676 32 49
Fragments arranged by size:
676 216 49 32 32
HincII GTy'rAC
Cuts at: 0 597 1005
Size: 597 408
MslI CAynn'nnrTG
Cuts at: 0 220 247 492 894 916 1005
Size: 220 27 245 402 22 89
Fragments arranged by size:
402 245 220 89 27 22
NcoI C'CATG_G
Cuts at: 0 65 425 1005
Size: 65 360 580
Fragments arranged by size:
580 360 65
NspI r_CATG'y
Cuts at: 0 732 1005
Size: 732 273
PciI A'CATG_T
Cuts at: 0 728 1005
Size: 728 277
PstI C_TGCA'G
Cuts at: 0 799 1005
Size: 799 206
SacI G_AGCT'C
Cuts at: 0 143 1005
Size: 143 862
SfcI C'TryA_G
Cuts at: 0 795 1005
Size: 795 210
SspI AAT'ATT
Cuts at: 0 996 1005
Size: 996 9
StuI AGG'CCT
Cuts at: 0 784 1005
Size: 784 221
StyI C'CwwG_G
Cuts at: 0 65 261 311 425 1005
Size: 65 196 50 114 580
Fragments arranged by size:
580 196 114 65 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 58 1005
Size: 58 947
TatI w'GTAC_w
Cuts at: 0 496 1005
Size: 496 509
XbaI T'CTAG_A
Cuts at: 0 981 1005
Size: 981 24
XmnI GAAnn'nnTTC
Cuts at: 0 784 1005
Size: 784 221
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApoI AvaI
AvrII BamHI BanII BclI BmgBI Bpu10I BseRI BseYI
BsiHKAI Bsp1286I BspMI BstYI BtgI EaeI EarI EcoICRI
Eco57MI EcoRV FalI Hin4I HincII MslI NcoI NspI
PciI PstI SacI SfcI SspI StuI StyI TaqII
TatI XbaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI BaeI BanI
BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SwaI TstI Tth111I XcmI XhoI XmaI
ZraI