Restriction map for
pFN21AE5480
(Linear) (Six-base) MAPSORT of: KIEE5480.seq Check: 9803 from: 1 to: 993
KIEE5480.orf
With 182 enzymes: SgfI *
January 29, 2014 12:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 993
Size: 686 307
AflIII A'CryG_T
Cuts at: 0 917 993
Size: 917 76
AleI CACnn'nnGTG
Cuts at: 0 23 652 993
Size: 23 629 341
Fragments arranged by size:
629 341 23
AlwNI CAG_nnn'CTG
Cuts at: 0 490 993
Size: 490 503
AvaI C'yCGr_G
Cuts at: 0 325 993
Size: 325 668
BanII G_rGCy'C
Cuts at: 0 56 993
Size: 56 937
BclI T'GATC_A
Cuts at: 0 644 993
Size: 644 349
BlpI GC'TnA_GC
Cuts at: 0 250 993
Size: 250 743
BmrI ACTGGGnnnn_n'
Cuts at: 0 831 993
Size: 831 162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 993
Size: 613 380
Bpu10I CC'TnA_GC
Cuts at: 0 685 993
Size: 685 308
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 993
Size: 249 744
BsaAI yAC'GTr
Cuts at: 0 886 993
Size: 886 107
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 610 993
Size: 610 383
BseYI C'CCAG_C
Cuts at: 0 449 993
Size: 449 544
Bsp1286I G_dGCh'C
Cuts at: 0 56 993
Size: 56 937
BspHI T'CATG_A
Cuts at: 0 410 993
Size: 410 583
BsrDI GCAATG_nn'
Cuts at: 0 135 993
Size: 135 858
BstEII G'GTnAC_C
Cuts at: 0 196 750 993
Size: 196 554 243
Fragments arranged by size:
554 243 196
BstXI CCAn_nnnn'nTGG
Cuts at: 0 591 993
Size: 591 402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 779 993
Size: 779 214
BtsI GCAGTG_nn'
Cuts at: 0 556 647 833 993
Size: 556 91 186 160
Fragments arranged by size:
556 186 160 91
EaeI y'GGCC_r
Cuts at: 0 377 790 993
Size: 377 413 203
Fragments arranged by size:
413 377 203
EarI CTCTTCn'nnn_
Cuts at: 0 763 993
Size: 763 230
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 553 993
Size: 553 440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 686 993
Size: 613 73 307
Fragments arranged by size:
613 307 73
EcoO109I rG'GnC_Cy
Cuts at: 0 329 993
Size: 329 664
HindIII A'AGCT_T
Cuts at: 0 905 993
Size: 905 88
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 993
Size: 55 938
MscI TGG'CCA
Cuts at: 0 379 993
Size: 379 614
MslI CAynn'nnrTG
Cuts at: 0 23 652 993
Size: 23 629 341
Fragments arranged by size:
629 341 23
NspI r_CATG'y
Cuts at: 0 296 921 993
Size: 296 625 72
Fragments arranged by size:
625 296 72
PciI A'CATG_T
Cuts at: 0 917 993
Size: 917 76
SfcI C'TryA_G
Cuts at: 0 169 993
Size: 169 824
SmlI C'TyrA_G
Cuts at: 0 264 993
Size: 264 729
SnaBI TAC'GTA
Cuts at: 0 886 993
Size: 886 107
StuI AGG'CCT
Cuts at: 0 718 993
Size: 718 275
StyI C'CwwG_G
Cuts at: 0 440 993
Size: 440 553
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 441 993
Size: 316 125 552
Fragments arranged by size:
552 316 125
TatI w'GTAC_w
Cuts at: 0 182 993
Size: 182 811
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 993
Size: 265 32 696
Fragments arranged by size:
696 265 32
Tth111I GACn'n_nGTC
Cuts at: 0 607 993
Size: 607 386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 489 993
Size: 489 504
XmnI GAAnn'nnTTC
Cuts at: 0 718 868 993
Size: 718 150 125
Fragments arranged by size:
718 150 125
Enzymes that do cut:
AcuI AflIII AleI AlwNI AvaI BanII BclI BlpI
BmrI BpmI Bpu10I BpuEI BsaAI BseRI BseYI Bsp1286I
BspHI BsrDI BstEII BstXI BtgZI BtsI EaeI EarI
EciI Eco57MI EcoO109I HindIII MmeI MscI MslI NspI
PciI SfcI SmlI SnaBI StuI StyI TaqII TatI
TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI BmtI
BplI BsaI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI
SphI SrfI SspI SwaI XbaI XhoI XmaI ZraI