Restriction map for
pFN21AE5479
(Linear) (Six-base) MAPSORT of: KIEE5479.seq Check: 6179 from: 1 to: 993
KIEE5479.orf
With 182 enzymes: SgfI *
January 29, 2014 12:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 993
Size: 727 266
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 993
Size: 294 699
AflIII A'CryG_T
Cuts at: 0 218 993
Size: 218 775
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 953 985 993
Size: 953 32 8
Fragments arranged by size:
953 32 8
AlwNI CAG_nnn'CTG
Cuts at: 0 75 993
Size: 75 918
ApaLI G'TGCA_C
Cuts at: 0 324 993
Size: 324 669
ApoI r'AATT_y
Cuts at: 0 886 993
Size: 886 107
AvaI C'yCGr_G
Cuts at: 0 411 993
Size: 411 582
BanI G'GyrC_C
Cuts at: 0 28 993
Size: 28 965
BanII G_rGCy'C
Cuts at: 0 193 808 993
Size: 193 615 185
Fragments arranged by size:
615 193 185
BbsI GAAGACnn'nnnn_
Cuts at: 0 741 946 993
Size: 741 205 47
Fragments arranged by size:
741 205 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 261 295 993
Size: 261 34 698
Fragments arranged by size:
698 261 34
BclI T'GATC_A
Cuts at: 0 470 993
Size: 470 523
BglI GCCn_nnn'nGGC
Cuts at: 0 352 993
Size: 352 641
BglII A'GATC_T
Cuts at: 0 209 993
Size: 209 784
Bme1580I G_kGCm'C
Cuts at: 0 328 993
Size: 328 665
BmgBI CAC'GTC
Cuts at: 0 399 993
Size: 399 594
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 244 265 409 474 723 993
Size: 168 76 21 144 65 249 270
Fragments arranged by size:
270 249 168 144 76 65 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 993
Size: 538 455
BsaAI yAC'GTr
Cuts at: 0 219 369 993
Size: 219 150 624
Fragments arranged by size:
624 219 150
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 953 983 993
Size: 953 30 10
Fragments arranged by size:
953 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 247 993
Size: 76 171 746
Fragments arranged by size:
746 171 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 993
Size: 549 444
BsiEI CG_ry'CG
Cuts at: 0 363 993
Size: 363 630
BsiHKAI G_wGCw'C
Cuts at: 0 193 328 808 993
Size: 193 135 480 185
Fragments arranged by size:
480 193 185 135
BsmI GAATG_Cn'
Cuts at: 0 900 993
Size: 900 93
Bsp1286I G_dGCh'C
Cuts at: 0 193 328 808 993
Size: 193 135 480 185
Fragments arranged by size:
480 193 185 135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 83 727 993
Size: 83 644 266
Fragments arranged by size:
644 266 83
BsrFI r'CCGG_y
Cuts at: 0 694 993
Size: 694 299
BsrGI T'GTAC_A
Cuts at: 0 830 993
Size: 830 163
BstAPI GCAn_nnn'nTGC
Cuts at: 0 75 993
Size: 75 918
BstEII G'GTnAC_C
Cuts at: 0 741 993
Size: 741 252
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 993
Size: 243 750
BstYI r'GATC_y
Cuts at: 0 209 993
Size: 209 784
Bsu36I CC'TnA_GG
Cuts at: 0 874 993
Size: 874 119
BtgI C'CryG_G
Cuts at: 0 53 350 737 993
Size: 53 297 387 256
Fragments arranged by size:
387 297 256 53
BtsI GCAGTG_nn'
Cuts at: 0 578 993
Size: 578 415
DraIII CAC_nnn'GTG
Cuts at: 0 332 993
Size: 332 661
EaeI y'GGCC_r
Cuts at: 0 128 371 635 689 993
Size: 128 243 264 54 304
Fragments arranged by size:
304 264 243 128 54
EarI CTCTTCn'nnn_
Cuts at: 0 85 94 993
Size: 85 9 899
Fragments arranged by size:
899 85 9
EcoICRI GAG'CTC
Cuts at: 0 191 806 993
Size: 191 615 187
Fragments arranged by size:
615 191 187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 244 265 294 409 474 723 993
Size: 168 76 21 29 115 65 249 270
Fragments arranged by size:
270 249 168 115 76 65 29 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 915 993
Size: 915 78
FspI TGC'GCA
Cuts at: 0 656 679 993
Size: 656 23 314
Fragments arranged by size:
656 314 23
FspAI rTGC'GCAy
Cuts at: 0 656 993
Size: 656 337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 833 993
Size: 536 297 160
Fragments arranged by size:
536 297 160
MscI TGG'CCA
Cuts at: 0 130 373 637 993
Size: 130 243 264 356
Fragments arranged by size:
356 264 243 130
MslI CAynn'nnrTG
Cuts at: 0 40 466 619 993
Size: 40 426 153 374
Fragments arranged by size:
426 374 153 40
MspA1I CmG'CkG
Cuts at: 0 387 429 993
Size: 387 42 564
Fragments arranged by size:
564 387 42
NcoI C'CATG_G
Cuts at: 0 350 993
Size: 350 643
NdeI CA'TA_TG
Cuts at: 0 651 993
Size: 651 342
PasI CC'CwG_GG
Cuts at: 0 61 993
Size: 61 932
PflMI CCAn_nnn'nTGG
Cuts at: 0 432 993
Size: 432 561
PfoI T'CCnGG_A
Cuts at: 0 799 993
Size: 799 194
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 953 985 993
Size: 953 32 8
Fragments arranged by size:
953 32 8
PstI C_TGCA'G
Cuts at: 0 71 585 993
Size: 71 514 408
Fragments arranged by size:
514 408 71
PvuII CAG'CTG
Cuts at: 0 429 993
Size: 429 564
SacI G_AGCT'C
Cuts at: 0 193 808 993
Size: 193 615 185
Fragments arranged by size:
615 193 185
SapI GCTCTTCn'nnn_
Cuts at: 0 85 993
Size: 85 908
SfcI C'TryA_G
Cuts at: 0 67 581 993
Size: 67 514 412
Fragments arranged by size:
514 412 67
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 352 993
Size: 352 641
SmaI CCC'GGG
Cuts at: 0 413 993
Size: 413 580
SmlI C'TyrA_G
Cuts at: 0 553 993
Size: 553 440
StyI C'CwwG_G
Cuts at: 0 350 993
Size: 350 643
TatI w'GTAC_w
Cuts at: 0 176 830 993
Size: 176 654 163
Fragments arranged by size:
654 176 163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 555 585 993
Size: 555 30 408
Fragments arranged by size:
555 408 30
XmaI C'CCGG_G
Cuts at: 0 411 993
Size: 411 582
XmnI GAAnn'nnTTC
Cuts at: 0 526 993
Size: 526 467
Enzymes that do cut:
AarI AcuI AflIII AloI AlwNI ApaLI ApoI AvaI
BanI BanII BbsI BcgI BclI BglI BglII Bme1580I
BmgBI BpmI BpuEI BsaAI BsaXI BseRI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrFI BsrGI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI
EcoICRI Eco57MI FalI FspI FspAI MmeI MscI MslI
MspA1I NcoI NdeI PasI PflMI PfoI PpiI PstI
PvuII SacI SapI SfcI SfiI SmaI SmlI StyI
TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BciVI BfrBI BlpI BmrI BmtI
BplI Bpu10I BsaI BsaBI BsaHI BsaWI BseYI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BssHII BssSI BstBI
BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoNI EcoO109I EcoRI EcoRV FseI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XbaI XhoI ZraI