(Linear) (Six-base) MAPSORT of: KIEE5478.seq  Check: 6941  from: 1  to: 990



KIEE5478.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     468     622     990 
   Size:        468     154     368

  Fragments arranged by size: 

                 468     368     154
 
AgeI A'CCGG_T

Cuts at:      0     410     990 
   Size:        410     580
 
AlwNI CAG_nnn'CTG

Cuts at:      0     123     990 
   Size:        123     867
 
BanII G_rGCy'C

Cuts at:      0     948     990 
   Size:        948      42
 
BglI GCCn_nnn'nGGC

Cuts at:      0     502     990 
   Size:        502     488
 
BglII A'GATC_T

Cuts at:      0     255     990 
   Size:        255     735
 
Bme1580I G_kGCm'C

Cuts at:      0     507     990 
   Size:        507     483
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     561     990 
   Size:        561     429
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     736     942     990 
   Size:        269     467     206      48

  Fragments arranged by size: 

                 467     269     206      48
 
BsaWI w'CCGG_w

Cuts at:      0     410     990 
   Size:        410     580
 
Bsp1286I G_dGCh'C

Cuts at:      0     507     948     990 
   Size:        507     441      42

  Fragments arranged by size: 

                 507     441      42
 
BspHI T'CATG_A

Cuts at:      0     452     990 
   Size:        452     538
 
BsrFI r'CCGG_y

Cuts at:      0     410     990 
   Size:        410     580
 
BsrGI T'GTAC_A

Cuts at:      0     373     990 
   Size:        373     617
 
BstYI r'GATC_y

Cuts at:      0     119     255     990 
   Size:        119     136     735

  Fragments arranged by size: 

                 735     136     119
 
BtsI GCAGTG_nn'

Cuts at:      0     401     990 
   Size:        401     589
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     638     673     990 
   Size:        638      35     317

  Fragments arranged by size: 

                 638     317      35
 
EaeI y'GGCC_r

Cuts at:      0     494     638     990 
   Size:        494     144     352

  Fragments arranged by size: 

                 494     352     144
 
EarI CTCTTCn'nnn_

Cuts at:      0     802     990 
   Size:        802     188
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     468     622     990 
   Size:        468     154     368

  Fragments arranged by size: 

                 468     368     154
 
EcoO109I rG'GnC_Cy

Cuts at:      0     488     990 
   Size:        488     502
 
EcoRV GAT'ATC

Cuts at:      0     869     990 
   Size:        869     121
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     102     990 
   Size:        102     888
 
MscI TGG'CCA

Cuts at:      0     640     990 
   Size:        640     350
 
MslI CAynn'nnrTG

Cuts at:      0     220     634     823     990 
   Size:        220     414     189     167

  Fragments arranged by size: 

                 414     220     189     167
 
MspA1I CmG'CkG

Cuts at:      0     886     990 
   Size:        886     104
 
NspI r_CATG'y

Cuts at:      0     828     990 
   Size:        828     162
 
PfoI T'CCnGG_A

Cuts at:      0     197     990 
   Size:        197     793
 
PpuMI rG'GwC_Cy

Cuts at:      0     488     990 
   Size:        488     502
 
SmlI C'TyrA_G

Cuts at:      0     284     751     921     990 
   Size:        284     467     170      69

  Fragments arranged by size: 

                 467     284     170      69
 
SpeI A'CTAG_T

Cuts at:      0     507     957     990 
   Size:        507     450      33

  Fragments arranged by size: 

                 507     450      33
 
SspI AAT'ATT

Cuts at:      0     708     815     990 
   Size:        708     107     175

  Fragments arranged by size: 

                 708     175     107
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     323     359     990 
   Size:        323      36     631

  Fragments arranged by size: 

                 631     323      36
 
TatI w'GTAC_w

Cuts at:      0     373     437     990 
   Size:        373      64     553

  Fragments arranged by size: 

                 553     373      64
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     648     733     990 
   Size:        648      85     257

  Fragments arranged by size: 

                 648     257      85
 

 Enzymes that do cut:

     AcuI     AgeI    AlwNI    BanII     BglI    BglII Bme1580I     BmrI
    BpuEI    BsaWI Bsp1286I    BspHI    BsrFI    BsrGI    BstYI     BtsI
    CspCI     EaeI     EarI  Eco57MI EcoO109I    EcoRV     FalI     MscI
     MslI   MspA1I     NspI     PfoI    PpuMI     SmlI     SpeI     SspI
    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI    BmgBI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     StuI
     StyI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI