(Linear) (Six-base) MAPSORT of: KIEE5476.seq  Check: 3888  from: 1  to: 987



KIEE5476.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      63     987 
   Size:         63     924
 
AflIII A'CryG_T

Cuts at:      0     647     987 
   Size:        647     340
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     512     987 
   Size:        512     475
 
ApaI G_GGCC'C

Cuts at:      0     587     987 
   Size:        587     400
 
ApoI r'AATT_y

Cuts at:      0     106     987 
   Size:        106     881
 
BanII G_rGCy'C

Cuts at:      0     125     387     587     801     987 
   Size:        125     262     200     214     186

  Fragments arranged by size: 

                 262     214     200     186     125
 
BclI T'GATC_A

Cuts at:      0     593     987 
   Size:        593     394
 
BlpI GC'TnA_GC

Cuts at:      0     371     987 
   Size:        371     616
 
Bme1580I G_kGCm'C

Cuts at:      0     587     833     987 
   Size:        587     246     154

  Fragments arranged by size: 

                 587     246     154
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     987 
   Size:        210     777
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     768     987 
   Size:        768     219
 
BsaAI yAC'GTr

Cuts at:      0     650     987 
   Size:        650     337
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     544     987 
   Size:        544     443
 
BseYI C'CCAG_C

Cuts at:      0     117     987 
   Size:        117     870
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     448     624     782     987 
   Size:         35     413     176     158     205

  Fragments arranged by size: 

                 413     205     176     158      35
 
BsiHKAI G_wGCw'C

Cuts at:      0     387     987 
   Size:        387     600
 
BsmI GAATG_Cn'

Cuts at:      0      58     537     987 
   Size:         58     479     450

  Fragments arranged by size: 

                 479     450      58
 
Bsp1286I G_dGCh'C

Cuts at:      0     125     387     587     801     833     987 
   Size:        125     262     200     214      32     154

  Fragments arranged by size: 

                 262     214     200     154     125      32
 
BspHI T'CATG_A

Cuts at:      0     479     987 
   Size:        479     508
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      63     987 
   Size:         63     924
 
BsrDI GCAATG_nn'

Cuts at:      0     317     987 
   Size:        317     670
 
EaeI y'GGCC_r

Cuts at:      0     263     446     987 
   Size:        263     183     541

  Fragments arranged by size: 

                 541     263     183
 
EarI CTCTTCn'nnn_

Cuts at:      0     944     987 
   Size:        944      43
 
EcoICRI GAG'CTC

Cuts at:      0     385     987 
   Size:        385     602
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     987 
   Size:        210     777
 
EcoO109I rG'GnC_Cy

Cuts at:      0     752     987 
   Size:        752     235
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     474     506     987 
   Size:        474      32     481

  Fragments arranged by size: 

                 481     474      32
 
MscI TGG'CCA

Cuts at:      0     265     448     987 
   Size:        265     183     539

  Fragments arranged by size: 

                 539     265     183
 
MslI CAynn'nnrTG

Cuts at:      0     424     494     987 
   Size:        424      70     493

  Fragments arranged by size: 

                 493     424      70
 
NspI r_CATG'y

Cuts at:      0     204     987 
   Size:        204     783
 
PpuMI rG'GwC_Cy

Cuts at:      0     752     987 
   Size:        752     235
 
PspOMI G'GGCC_C

Cuts at:      0     583     987 
   Size:        583     404
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     134     166     987 
   Size:        134      32     821

  Fragments arranged by size: 

                 821     134      32
 
SacI G_AGCT'C

Cuts at:      0     387     987 
   Size:        387     600
 
SfcI C'TryA_G

Cuts at:      0     872     987 
   Size:        872     115
 
SmlI C'TyrA_G

Cuts at:      0     747     987 
   Size:        747     240
 
StyI C'CwwG_G

Cuts at:      0      71     987 
   Size:         71     916
 
TatI w'GTAC_w

Cuts at:      0     740     987 
   Size:        740     247
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     234     763     987 
   Size:        234     529     224

  Fragments arranged by size: 

                 529     234     224
 

 Enzymes that do cut:

     AarI   AflIII     AlfI     ApaI     ApoI    BanII     BclI     BlpI
 Bme1580I     BpmI    BpuEI    BsaAI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrDI     EaeI     EarI  EcoICRI
  Eco57MI EcoO109I    Hin4I     MscI     MslI     NspI    PpuMI   PspOMI
     PsrI     SacI     SfcI     SmlI     StyI     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI
    BsiWI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI