Restriction map for
pFN21AE5475
(Linear) (Six-base) MAPSORT of: KIEE5475.seq Check: 9433 from: 1 to: 984
KIEE5475.orf
With 182 enzymes: SgfI *
January 29, 2014 12:02 ..
AccI GT'mk_AC
Cuts at: 0 489 984
Size: 489 495
AflIII A'CryG_T
Cuts at: 0 533 984
Size: 533 451
ApaLI G'TGCA_C
Cuts at: 0 454 984
Size: 454 530
ApoI r'AATT_y
Cuts at: 0 25 984
Size: 25 959
AseI AT'TA_AT
Cuts at: 0 802 984
Size: 802 182
BanII G_rGCy'C
Cuts at: 0 163 984
Size: 163 821
BbsI GAAGACnn'nnnn_
Cuts at: 0 321 984
Size: 321 663
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 395 429 984
Size: 395 34 555
Fragments arranged by size:
555 395 34
Bme1580I G_kGCm'C
Cuts at: 0 458 984
Size: 458 526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 984
Size: 32 952
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 437 467 984
Size: 437 30 517
Fragments arranged by size:
517 437 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 984
Size: 112 872
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 367 984
Size: 341 26 617
Fragments arranged by size:
617 341 26
BsiHKAI G_wGCw'C
Cuts at: 0 458 984
Size: 458 526
Bsp1286I G_dGCh'C
Cuts at: 0 163 458 984
Size: 163 295 526
Fragments arranged by size:
526 295 163
BspHI T'CATG_A
Cuts at: 0 398 984
Size: 398 586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 718 984
Size: 718 266
BsrDI GCAATG_nn'
Cuts at: 0 21 984
Size: 21 963
BsrGI T'GTAC_A
Cuts at: 0 482 984
Size: 482 502
BstYI r'GATC_y
Cuts at: 0 51 984
Size: 51 933
BtsI GCAGTG_nn'
Cuts at: 0 215 984
Size: 215 769
DraIII CAC_nnn'GTG
Cuts at: 0 462 984
Size: 462 522
DrdI GACnn_nn'nnGTC
Cuts at: 0 523 984
Size: 523 461
EaeI y'GGCC_r
Cuts at: 0 434 984
Size: 434 550
EarI CTCTTCn'nnn_
Cuts at: 0 301 680 984
Size: 301 379 304
Fragments arranged by size:
379 304 301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 806 984
Size: 527 279 178
Fragments arranged by size:
527 279 178
MscI TGG'CCA
Cuts at: 0 436 984
Size: 436 548
MslI CAynn'nnrTG
Cuts at: 0 130 220 727 832 984
Size: 130 90 507 105 152
Fragments arranged by size:
507 152 130 105 90
MspA1I CmG'CkG
Cuts at: 0 381 984
Size: 381 603
NspI r_CATG'y
Cuts at: 0 537 984
Size: 537 447
PciI A'CATG_T
Cuts at: 0 533 984
Size: 533 451
PflMI CCAn_nnn'nTGG
Cuts at: 0 499 984
Size: 499 485
SapI GCTCTTCn'nnn_
Cuts at: 0 301 984
Size: 301 683
SmlI C'TyrA_G
Cuts at: 0 47 984
Size: 47 937
StyI C'CwwG_G
Cuts at: 0 155 984
Size: 155 829
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 120 243 984
Size: 120 123 741
Fragments arranged by size:
741 123 120
TatI w'GTAC_w
Cuts at: 0 170 482 984
Size: 170 312 502
Fragments arranged by size:
502 312 170
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 36 68 437 469 541 572 573 604
Size: 36 32 369 32 72 31 1 31
Cuts at: 604 984
Size: 380
Fragments arranged by size:
380 369 72 36 32 32 31 31
1
Enzymes that do cut:
AccI AflIII ApaLI ApoI AseI BanII BbsI BcgI
Bme1580I BpuEI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BsrGI BstYI BtsI DraIII DrdI EaeI
EarI MmeI MscI MslI MspA1I NspI PciI PflMI
SapI SmlI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI EagI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI