Restriction map for
pFN21AE5474
(Linear) (Six-base) MAPSORT of: KIEE5474.seq Check: 7558 from: 1 to: 981
KIEE5474.orf
With 182 enzymes: SgfI *
January 29, 2014 12:02 ..
AleI CACnn'nnGTG
Cuts at: 0 754 981
Size: 754 227
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 464 981
Size: 464 517
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 67 100 981
Size: 67 33 881
Fragments arranged by size:
881 67 33
BamHI G'GATC_C
Cuts at: 0 262 948 981
Size: 262 686 33
Fragments arranged by size:
686 262 33
BanII G_rGCy'C
Cuts at: 0 915 981
Size: 915 66
BfrBI ATG'CAT
Cuts at: 0 305 981
Size: 305 676
BglII A'GATC_T
Cuts at: 0 212 981
Size: 212 769
BmrI ACTGGGnnnn_n'
Cuts at: 0 406 665 981
Size: 406 259 316
Fragments arranged by size:
406 316 259
BmtI G_CTAG'C
Cuts at: 0 971 981
Size: 971 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 485 981
Size: 229 256 496
Fragments arranged by size:
496 256 229
BsaI GGTCTCn'nnnn_
Cuts at: 0 840 981
Size: 840 141
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 15 45 202 232 981
Size: 15 30 157 30 749
Fragments arranged by size:
749 157 30 30 15
BseYI C'CCAG_C
Cuts at: 0 45 981
Size: 45 936
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 460 913 981
Size: 324 136 453 68
Fragments arranged by size:
453 324 136 68
Bsp1286I G_dGCh'C
Cuts at: 0 915 981
Size: 915 66
BsrDI GCAATG_nn'
Cuts at: 0 562 862 981
Size: 562 300 119
Fragments arranged by size:
562 300 119
BsrFI r'CCGG_y
Cuts at: 0 428 981
Size: 428 553
BstXI CCAn_nnnn'nTGG
Cuts at: 0 831 981
Size: 831 150
BstYI r'GATC_y
Cuts at: 0 212 262 948 981
Size: 212 50 686 33
Fragments arranged by size:
686 212 50 33
BtgI C'CryG_G
Cuts at: 0 482 981
Size: 482 499
DraIII CAC_nnn'GTG
Cuts at: 0 347 981
Size: 347 634
EaeI y'GGCC_r
Cuts at: 0 386 426 620 671 796 981
Size: 386 40 194 51 125 185
Fragments arranged by size:
386 194 185 125 51 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 24 981
Size: 24 957
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 485 981
Size: 229 256 496
Fragments arranged by size:
496 256 229
EcoO109I rG'GnC_Cy
Cuts at: 0 659 981
Size: 659 322
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 514 981
Size: 514 467
FspI TGC'GCA
Cuts at: 0 892 981
Size: 892 89
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 272 304 981
Size: 272 32 677
Fragments arranged by size:
677 272 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 981
Size: 240 741
MscI TGG'CCA
Cuts at: 0 388 622 673 981
Size: 388 234 51 308
Fragments arranged by size:
388 308 234 51
MslI CAynn'nnrTG
Cuts at: 0 139 481 586 754 981
Size: 139 342 105 168 227
Fragments arranged by size:
342 227 168 139 105
NaeI GCC'GGC
Cuts at: 0 430 981
Size: 430 551
NcoI C'CATG_G
Cuts at: 0 482 981
Size: 482 499
NgoMIV G'CCGG_C
Cuts at: 0 428 981
Size: 428 553
NheI G'CTAG_C
Cuts at: 0 967 981
Size: 967 14
NsiI A_TGCA'T
Cuts at: 0 307 981
Size: 307 674
SfcI C'TryA_G
Cuts at: 0 70 981
Size: 70 911
StuI AGG'CCT
Cuts at: 0 724 981
Size: 724 257
StyI C'CwwG_G
Cuts at: 0 327 482 981
Size: 327 155 499
Fragments arranged by size:
499 327 155
TatI w'GTAC_w
Cuts at: 0 179 840 922 981
Size: 179 661 82 59
Fragments arranged by size:
661 179 82 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3 15 35 47 981
Size: 3 12 20 12 934
Fragments arranged by size:
934 20 12 12 3
Enzymes that do cut:
AleI AlfI BaeI BamHI BanII BfrBI BglII BmrI
BmtI BpmI BsaI BsaXI BseYI BsgI Bsp1286I BsrDI
BsrFI BstXI BstYI BtgI DraIII EaeI EciI Eco57MI
EcoO109I FalI FspI Hin4I MmeI MscI MslI NaeI
NcoI NgoMIV NheI NsiI SfcI StuI StyI TatI
TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BanI BbeI BbsI
BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI
BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI
EarI EcoICRI EcoNI EcoRI EcoRV FseI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MspA1I
NarI NdeI NotI NruI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI