Restriction map for
pFN21AE5473
(Linear) (Six-base) MAPSORT of: KIEE5473.seq Check: 1197 from: 1 to: 981
KIEE5473.orf
With 182 enzymes: SgfI *
January 29, 2014 12:02 ..
AccI GT'mk_AC
Cuts at: 0 262 274 626 981
Size: 262 12 352 355
Fragments arranged by size:
355 352 262 12
Acc65I G'GTAC_C
Cuts at: 0 445 981
Size: 445 536
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 930 981
Size: 722 208 51
Fragments arranged by size:
722 208 51
AleI CACnn'nnGTG
Cuts at: 0 37 981
Size: 37 944
ApoI r'AATT_y
Cuts at: 0 76 981
Size: 76 905
BanI G'GyrC_C
Cuts at: 0 445 981
Size: 445 536
BanII G_rGCy'C
Cuts at: 0 342 981
Size: 342 639
BbsI GAAGACnn'nnnn_
Cuts at: 0 750 981
Size: 750 231
BclI T'GATC_A
Cuts at: 0 884 981
Size: 884 97
BlpI GC'TnA_GC
Cuts at: 0 341 981
Size: 341 640
BmrI ACTGGGnnnn_n'
Cuts at: 0 102 479 981
Size: 102 377 502
Fragments arranged by size:
502 377 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 981
Size: 654 327
BsmI GAATG_Cn'
Cuts at: 0 382 513 674 754 981
Size: 382 131 161 80 227
Fragments arranged by size:
382 227 161 131 80
Bsp1286I G_dGCh'C
Cuts at: 0 342 981
Size: 342 639
BspHI T'CATG_A
Cuts at: 0 15 981
Size: 15 966
BsrDI GCAATG_nn'
Cuts at: 0 430 981
Size: 430 551
BsrGI T'GTAC_A
Cuts at: 0 610 981
Size: 610 371
BstXI CCAn_nnnn'nTGG
Cuts at: 0 288 981
Size: 288 693
BstZ17I GTA'TAC
Cuts at: 0 263 275 981
Size: 263 12 706
Fragments arranged by size:
706 263 12
BtgI C'CryG_G
Cuts at: 0 38 395 981
Size: 38 357 586
Fragments arranged by size:
586 357 38
BtsI GCAGTG_nn'
Cuts at: 0 892 981
Size: 892 89
DrdI GACnn_nn'nnGTC
Cuts at: 0 622 981
Size: 622 359
EaeI y'GGCC_r
Cuts at: 0 290 981
Size: 290 691
EarI CTCTTCn'nnn_
Cuts at: 0 799 981
Size: 799 182
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 526 981
Size: 526 455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 722 930 981
Size: 654 68 208 51
Fragments arranged by size:
654 208 68 51
EcoRI G'AATT_C
Cuts at: 0 76 981
Size: 76 905
EcoRV GAT'ATC
Cuts at: 0 933 981
Size: 933 48
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 658 981
Size: 658 323
HincII GTy'rAC
Cuts at: 0 702 981
Size: 702 279
KpnI G_GTAC'C
Cuts at: 0 449 981
Size: 449 532
MscI TGG'CCA
Cuts at: 0 292 981
Size: 292 689
MslI CAynn'nnrTG
Cuts at: 0 37 49 548 981
Size: 37 12 499 433
Fragments arranged by size:
499 433 37 12
MspA1I CmG'CkG
Cuts at: 0 345 981
Size: 345 636
NcoI C'CATG_G
Cuts at: 0 395 981
Size: 395 586
NdeI CA'TA_TG
Cuts at: 0 402 981
Size: 402 579
PvuII CAG'CTG
Cuts at: 0 345 981
Size: 345 636
SapI GCTCTTCn'nnn_
Cuts at: 0 799 981
Size: 799 182
ScaI AGT'ACT
Cuts at: 0 665 981
Size: 665 316
SfcI C'TryA_G
Cuts at: 0 912 981
Size: 912 69
StuI AGG'CCT
Cuts at: 0 828 943 981
Size: 828 115 38
Fragments arranged by size:
828 115 38
StyI C'CwwG_G
Cuts at: 0 176 323 395 981
Size: 176 147 72 586
Fragments arranged by size:
586 176 147 72
TatI w'GTAC_w
Cuts at: 0 221 466 610 663 981
Size: 221 245 144 53 318
Fragments arranged by size:
318 245 221 144 53
XmnI GAAnn'nnTTC
Cuts at: 0 198 981
Size: 198 783
Enzymes that do cut:
AccI Acc65I AcuI AleI ApoI BanI BanII BbsI
BclI BlpI BmrI BpmI BsmI Bsp1286I BspHI BsrDI
BsrGI BstXI BstZ17I BtgI BtsI DrdI EaeI EarI
EciI Eco57MI EcoRI EcoRV FalI HincII KpnI MscI
MslI MspA1I NcoI NdeI PvuII SapI ScaI SfcI
StuI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BfrBI BglI BglII Bme1580I BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EcoICRI
EcoNI EcoO109I FseI FspI FspAI HaeII Hin4I HindIII
HpaI KasI MfeI MluI MmeI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI