Restriction map for
pFN21AE5471
(Linear) (Six-base) MAPSORT of: KIEE5471.seq Check: 5582 from: 1 to: 969
KIEE5471.orf
With 182 enzymes: SgfI *
January 29, 2014 12:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 969
Size: 450 519
AflII C'TTAA_G
Cuts at: 0 459 969
Size: 459 510
AleI CACnn'nnGTG
Cuts at: 0 64 969
Size: 64 905
AlwNI CAG_nnn'CTG
Cuts at: 0 827 966 969
Size: 827 139 3
Fragments arranged by size:
827 139 3
AvaI C'yCGr_G
Cuts at: 0 480 969
Size: 480 489
BbsI GAAGACnn'nnnn_
Cuts at: 0 342 483 969
Size: 342 141 486
Fragments arranged by size:
486 342 141
BclI T'GATC_A
Cuts at: 0 617 969
Size: 617 352
BlpI GC'TnA_GC
Cuts at: 0 187 969
Size: 187 782
BmgBI CAC'GTC
Cuts at: 0 800 969
Size: 800 169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 969
Size: 247 722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 969
Size: 643 326
BsaI GGTCTCn'nnnn_
Cuts at: 0 75 556 969
Size: 75 481 413
Fragments arranged by size:
481 413 75
BsaWI w'CCGG_w
Cuts at: 0 73 969
Size: 73 896
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 535 565 969
Size: 535 30 404
Fragments arranged by size:
535 404 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 519 969
Size: 519 450
BseYI C'CCAG_C
Cuts at: 0 709 969
Size: 709 260
BspEI T'CCGG_A
Cuts at: 0 73 969
Size: 73 896
BsrDI GCAATG_nn'
Cuts at: 0 693 969
Size: 693 276
BsrFI r'CCGG_y
Cuts at: 0 868 969
Size: 868 101
BstEII G'GTnAC_C
Cuts at: 0 51 166 969
Size: 51 115 803
Fragments arranged by size:
803 115 51
BstYI r'GATC_y
Cuts at: 0 602 969
Size: 602 367
BtgI C'CryG_G
Cuts at: 0 539 969
Size: 539 430
EaeI y'GGCC_r
Cuts at: 0 119 870 969
Size: 119 751 99
Fragments arranged by size:
751 119 99
EarI CTCTTCn'nnn_
Cuts at: 0 292 397 906 969
Size: 292 105 509 63
Fragments arranged by size:
509 292 105 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 450 969
Size: 247 203 519
Fragments arranged by size:
519 247 203
EcoO109I rG'GnC_Cy
Cuts at: 0 705 969
Size: 705 264
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 72 570 961 969
Size: 72 498 391 8
Fragments arranged by size:
498 391 72 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 639 671 969
Size: 639 32 298
Fragments arranged by size:
639 298 32
HincII GTy'rAC
Cuts at: 0 576 969
Size: 576 393
HindIII A'AGCT_T
Cuts at: 0 462 969
Size: 462 507
MfeI C'AATT_G
Cuts at: 0 779 969
Size: 779 190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 827 969
Size: 557 270 142
Fragments arranged by size:
557 270 142
MslI CAynn'nnrTG
Cuts at: 0 64 969
Size: 64 905
NaeI GCC'GGC
Cuts at: 0 870 969
Size: 870 99
NgoMIV G'CCGG_C
Cuts at: 0 868 969
Size: 868 101
PasI CC'CwG_GG
Cuts at: 0 901 969
Size: 901 68
PflMI CCAn_nnn'nTGG
Cuts at: 0 326 969
Size: 326 643
PfoI T'CCnGG_A
Cuts at: 0 826 969
Size: 826 143
PpuMI rG'GwC_Cy
Cuts at: 0 705 969
Size: 705 264
PsiI TTA'TAA
Cuts at: 0 263 969
Size: 263 706
SanDI GG'GwC_CC
Cuts at: 0 705 969
Size: 705 264
SapI GCTCTTCn'nnn_
Cuts at: 0 906 969
Size: 906 63
ScaI AGT'ACT
Cuts at: 0 553 969
Size: 553 416
SexAI A'CCwGG_T
Cuts at: 0 578 969
Size: 578 391
SfcI C'TryA_G
Cuts at: 0 208 969
Size: 208 761
SmlI C'TyrA_G
Cuts at: 0 459 480 658 969
Size: 459 21 178 311
Fragments arranged by size:
459 311 178 21
SspI AAT'ATT
Cuts at: 0 834 969
Size: 834 135
StyI C'CwwG_G
Cuts at: 0 99 427 969
Size: 99 328 542
Fragments arranged by size:
542 328 99
TatI w'GTAC_w
Cuts at: 0 551 879 969
Size: 551 328 90
Fragments arranged by size:
551 328 90
XhoI C'TCGA_G
Cuts at: 0 480 969
Size: 480 489
XmnI GAAnn'nnTTC
Cuts at: 0 235 553 969
Size: 235 318 416
Fragments arranged by size:
416 318 235
Enzymes that do cut:
AcuI AflII AleI AlwNI AvaI BbsI BclI BlpI
BmgBI BpmI BpuEI BsaI BsaWI BsaXI BseRI BseYI
BspEI BsrDI BsrFI BstEII BstYI BtgI EaeI EarI
Eco57MI EcoO109I FalI Hin4I HincII HindIII MfeI MmeI
MslI NaeI NgoMIV PasI PflMI PfoI PpuMI PsiI
SanDI SapI ScaI SexAI SfcI SmlI SspI StyI
TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BfrBI BglI BglII Bme1580I BmrI BmtI
BplI Bpu10I BsaAI BsaBI BsaHI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MluI MscI MspA1I NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TaqII TstI Tth111I XbaI XcmI XmaI
ZraI