(Linear) (Six-base) MAPSORT of: KIEE5467.seq  Check: 889  from: 1  to: 930



KIEE5467.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     724     930 
   Size:        724     206
 
AleI CACnn'nnGTG

Cuts at:      0     115     930 
   Size:        115     815
 
ApoI r'AATT_y

Cuts at:      0      73     930 
   Size:         73     857
 
AvaI C'yCGr_G

Cuts at:      0     684     930 
   Size:        684     246
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0       8      41     930 
   Size:          8      33     889

  Fragments arranged by size: 

                 889      33       8
 
BanII G_rGCy'C

Cuts at:      0      68     930 
   Size:         68     862
 
BbvCI CC'TCA_GC

Cuts at:      0     673     930 
   Size:        673     257
 
BglII A'GATC_T

Cuts at:      0     299     930 
   Size:        299     631
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     604     930 
   Size:         52     552     326

  Fragments arranged by size: 

                 552     326      52
 
Bpu10I CC'TnA_GC

Cuts at:      0     673     930 
   Size:        673     257
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     256     930 
   Size:        256     674
 
BsaWI w'CCGG_w

Cuts at:      0     389     930 
   Size:        389     541
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     625     909     930 
   Size:        625     284      21

  Fragments arranged by size: 

                 625     284      21
 
BseYI C'CCAG_C

Cuts at:      0     380     930 
   Size:        380     550
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     549     702     930 
   Size:        549     153     228

  Fragments arranged by size: 

                 549     228     153
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     930 
   Size:         68     862
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     930 
   Size:         68     862
 
BspHI T'CATG_A

Cuts at:      0     404     593     830     930 
   Size:        404     189     237     100

  Fragments arranged by size: 

                 404     237     189     100
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     724     930 
   Size:        724     206
 
BsrFI r'CCGG_y

Cuts at:      0     158     930 
   Size:        158     772
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     117     930 
   Size:        117     813
 
BstYI r'GATC_y

Cuts at:      0     299     930 
   Size:        299     631
 
BtsI GCAGTG_nn'

Cuts at:      0     493     930 
   Size:        493     437
 
EcoICRI GAG'CTC

Cuts at:      0      66     930 
   Size:         66     864
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     604     930 
   Size:         52     552     326

  Fragments arranged by size: 

                 552     326      52
 
EcoO109I rG'GnC_Cy

Cuts at:      0     677     930 
   Size:        677     253
 
MfeI C'AATT_G

Cuts at:      0     415     930 
   Size:        415     515
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     498     626     930 
   Size:        498     128     304

  Fragments arranged by size: 

                 498     304     128
 
MslI CAynn'nnrTG

Cuts at:      0     115     172     403     664     733     736     829     930 
   Size:        115      57     231     261      69       3      93     101

  Fragments arranged by size: 

                 261     231     115     101      93      69      57       3
            
 
NaeI GCC'GGC

Cuts at:      0     160     930 
   Size:        160     770
 
NgoMIV G'CCGG_C

Cuts at:      0     158     930 
   Size:        158     772
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     318     930 
   Size:        318     612
 
PfoI T'CCnGG_A

Cuts at:      0     581     930 
   Size:        581     349
 
PpuMI rG'GwC_Cy

Cuts at:      0     677     930 
   Size:        677     253
 
SacI G_AGCT'C

Cuts at:      0      68     930 
   Size:         68     862
 
ScaI AGT'ACT

Cuts at:      0     551     930 
   Size:        551     379
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     186     435     739     930 
   Size:        186     249     304     191

  Fragments arranged by size: 

                 304     249     191     186
 
TatI w'GTAC_w

Cuts at:      0     549     930 
   Size:        549     381
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     474     930 
   Size:        474     456
 

 Enzymes that do cut:

     AarI     AleI     ApoI     AvaI     BaeI    BanII    BbvCI    BglII
     BpmI   Bpu10I     BsaI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrFI    BstXI    BstYI     BtsI  EcoICRI
  Eco57MI EcoO109I     MfeI     MmeI     MslI     NaeI   NgoMIV    PflMI
     PfoI    PpuMI     SacI     ScaI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII    BamHI     BanI     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MscI   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI