Restriction map for
pFN21AE5466
(Linear) (Six-base) MAPSORT of: KIEE5466.seq Check: 6467 from: 1 to: 930
KIEE5466.orf
With 182 enzymes: SgfI *
January 29, 2014 12:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 930
Size: 727 203
AccI GT'mk_AC
Cuts at: 0 381 930
Size: 381 549
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 582 713 930
Size: 474 108 131 217
Fragments arranged by size:
474 217 131 108
AflII C'TTAA_G
Cuts at: 0 703 930
Size: 703 227
ApoI r'AATT_y
Cuts at: 0 619 930
Size: 619 311
BanII G_rGCy'C
Cuts at: 0 442 930
Size: 442 488
BbsI GAAGACnn'nnnn_
Cuts at: 0 81 930
Size: 81 849
BbvCI CC'TCA_GC
Cuts at: 0 222 930
Size: 222 708
BglII A'GATC_T
Cuts at: 0 587 930
Size: 587 343
BmrI ACTGGGnnnn_n'
Cuts at: 0 291 930
Size: 291 639
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 930
Size: 267 663
Bpu10I CC'TnA_GC
Cuts at: 0 222 930
Size: 222 708
BsaI GGTCTCn'nnnn_
Cuts at: 0 731 930
Size: 731 199
BsaBI GATnn'nnATC
Cuts at: 0 70 930
Size: 70 860
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 210 930
Size: 210 720
BseYI C'CCAG_C
Cuts at: 0 435 930
Size: 435 495
BsmI GAATG_Cn'
Cuts at: 0 686 930
Size: 686 244
Bsp1286I G_dGCh'C
Cuts at: 0 442 930
Size: 442 488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 405 727 930
Size: 405 322 203
Fragments arranged by size:
405 322 203
BsrDI GCAATG_nn'
Cuts at: 0 227 930
Size: 227 703
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 930
Size: 243 687
BstYI r'GATC_y
Cuts at: 0 587 930
Size: 587 343
Bsu36I CC'TnA_GG
Cuts at: 0 778 930
Size: 778 152
BtgI C'CryG_G
Cuts at: 0 350 930
Size: 350 580
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 282 317 533 568 796 831 930
Size: 282 35 216 35 228 35 99
Fragments arranged by size:
282 228 216 99 35 35 35
EaeI y'GGCC_r
Cuts at: 0 371 930
Size: 371 559
EarI CTCTTCn'nnn_
Cuts at: 0 601 754 930
Size: 601 153 176
Fragments arranged by size:
601 176 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 474 582 713 930
Size: 267 207 108 131 217
Fragments arranged by size:
267 217 207 131 108
EcoO109I rG'GnC_Cy
Cuts at: 0 57 930
Size: 57 873
EcoRI G'AATT_C
Cuts at: 0 619 930
Size: 619 311
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 718 930
Size: 718 212
HincII GTy'rAC
Cuts at: 0 207 382 930
Size: 207 175 548
Fragments arranged by size:
548 207 175
MscI TGG'CCA
Cuts at: 0 373 930
Size: 373 557
MslI CAynn'nnrTG
Cuts at: 0 172 403 679 763 769 847 930
Size: 172 231 276 84 6 78 83
Fragments arranged by size:
276 231 172 84 83 78 6
MspA1I CmG'CkG
Cuts at: 0 287 691 930
Size: 287 404 239
Fragments arranged by size:
404 287 239
NcoI C'CATG_G
Cuts at: 0 350 930
Size: 350 580
PpuMI rG'GwC_Cy
Cuts at: 0 57 930
Size: 57 873
PvuII CAG'CTG
Cuts at: 0 287 691 930
Size: 287 404 239
Fragments arranged by size:
404 287 239
SalI G'TCGA_C
Cuts at: 0 380 930
Size: 380 550
SapI GCTCTTCn'nnn_
Cuts at: 0 601 930
Size: 601 329
SexAI A'CCwGG_T
Cuts at: 0 104 930
Size: 104 826
SfcI C'TryA_G
Cuts at: 0 393 867 930
Size: 393 474 63
Fragments arranged by size:
474 393 63
SmlI C'TyrA_G
Cuts at: 0 703 930
Size: 703 227
SspI AAT'ATT
Cuts at: 0 126 930
Size: 126 804
StyI C'CwwG_G
Cuts at: 0 350 930
Size: 350 580
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 67 186 310 930
Size: 67 119 124 620
Fragments arranged by size:
620 124 119 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 316 348 930
Size: 316 32 582
Fragments arranged by size:
582 316 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 798 930
Size: 798 132
XmnI GAAnn'nnTTC
Cuts at: 0 684 930
Size: 684 246
Enzymes that do cut:
AarI AccI AcuI AflII ApoI BanII BbsI BbvCI
BglII BmrI BpmI Bpu10I BsaI BsaBI BseRI BseYI
BsmI Bsp1286I BspMI BsrDI BstXI BstYI Bsu36I BtgI
CspCI EaeI EarI Eco57MI EcoO109I EcoRI FalI HincII
MscI MslI MspA1I NcoI PpuMI PvuII SalI SapI
SexAI SfcI SmlI SspI StyI TaqII TstI XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI
BpuEI BsaAI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV
FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TatI Tth111I XbaI
XhoI XmaI ZraI