(Linear) (Six-base) MAPSORT of: KIEE5461.seq  Check: 1438  from: 1  to: 927



KIEE5461.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:01  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     397     927 
   Size:        397     530
 
AlwNI CAG_nnn'CTG

Cuts at:      0     413     927 
   Size:        413     514
 
ApoI r'AATT_y

Cuts at:      0     186     203     927 
   Size:        186      17     724

  Fragments arranged by size: 

                 724     186      17
 
AseI AT'TA_AT

Cuts at:      0     773     927 
   Size:        773     154
 
AvrII C'CTAG_G

Cuts at:      0     111     927 
   Size:        111     816
 
BamHI G'GATC_C

Cuts at:      0     674     927 
   Size:        674     253
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      46     927 
   Size:         46     881
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     927 
   Size:        142     785
 
BsaBI GATnn'nnATC

Cuts at:      0      76     589     733     927 
   Size:         76     513     144     194

  Fragments arranged by size: 

                 513     194     144      76
 
BsaWI w'CCGG_w

Cuts at:      0     182     927 
   Size:        182     745
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      44      74     927 
   Size:         44      30     853

  Fragments arranged by size: 

                 853      44      30
 
BseYI C'CCAG_C

Cuts at:      0     686     927 
   Size:        686     241
 
BspEI T'CCGG_A

Cuts at:      0     182     927 
   Size:        182     745
 
BsrGI T'GTAC_A

Cuts at:      0     767     927 
   Size:        767     160
 
BssSI C'ACGA_G

Cuts at:      0     544     927 
   Size:        544     383
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     413     927 
   Size:        413     514
 
BstYI r'GATC_y

Cuts at:      0     674     927 
   Size:        674     253
 
EaeI y'GGCC_r

Cuts at:      0     365     599     927 
   Size:        365     234     328

  Fragments arranged by size: 

                 365     328     234
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     927 
   Size:        142     785
 
HindIII A'AGCT_T

Cuts at:      0     890     927 
   Size:        890      37
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     530     927 
   Size:        530     397
 
MscI TGG'CCA

Cuts at:      0     367     927 
   Size:        367     560
 
MslI CAynn'nnrTG

Cuts at:      0      97     478     763     927 
   Size:         97     381     285     164

  Fragments arranged by size: 

                 381     285     164      97
 
MspA1I CmG'CkG

Cuts at:      0     755     927 
   Size:        755     172
 
NspI r_CATG'y

Cuts at:      0     102     762     927 
   Size:        102     660     165

  Fragments arranged by size: 

                 660     165     102
 
PvuII CAG'CTG

Cuts at:      0     755     927 
   Size:        755     172
 
SpeI A'CTAG_T

Cuts at:      0     423     927 
   Size:        423     504
 
StuI AGG'CCT

Cuts at:      0     703     927 
   Size:        703     224
 
StyI C'CwwG_G

Cuts at:      0     111     927 
   Size:        111     816
 
TatI w'GTAC_w

Cuts at:      0     767     819     927 
   Size:        767      52     108

  Fragments arranged by size: 

                 767     108      52
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     458     490     927 
   Size:        458      32     437

  Fragments arranged by size: 

                 458     437      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     904     927 
   Size:        904      23
 
XmnI GAAnn'nnTTC

Cuts at:      0     853     927 
   Size:        853      74
 

 Enzymes that do cut:

     AlfI    AlwNI     ApoI     AseI    AvrII    BamHI     BmrI     BpmI
    BsaBI    BsaWI    BsaXI    BseYI    BspEI    BsrGI    BssSI   BstAPI
    BstYI     EaeI  Eco57MI  HindIII     MmeI     MscI     MslI   MspA1I
     NspI    PvuII     SpeI     StuI     StyI     TatI     TstI  Tth111I
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI
    AsiSI     AvaI     BaeI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SphI     SrfI     SspI     SwaI    TaqII     XbaI     XcmI
     XhoI     XmaI     ZraI