Restriction map for
pFN21AE5460
(Linear) (Six-base) MAPSORT of: KIEE5460.seq Check: 2147 from: 1 to: 1002
KIEE5460.orf
With 182 enzymes: SgfI *
January 29, 2014 12:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 1002
Size: 438 564
AleI CACnn'nnGTG
Cuts at: 0 676 1002
Size: 676 326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 194 1002
Size: 194 808
ApoI r'AATT_y
Cuts at: 0 664 985 1002
Size: 664 321 17
Fragments arranged by size:
664 321 17
AseI AT'TA_AT
Cuts at: 0 914 1002
Size: 914 88
BanII G_rGCy'C
Cuts at: 0 265 1002
Size: 265 737
BbsI GAAGACnn'nnnn_
Cuts at: 0 227 1002
Size: 227 775
BciVI GTATCCnnnnn_n'
Cuts at: 0 584 1002
Size: 584 418
Bme1580I G_kGCm'C
Cuts at: 0 183 1002
Size: 183 819
BmgBI CAC'GTC
Cuts at: 0 759 1002
Size: 759 243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 1002
Size: 50 952
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1002
Size: 640 362
BsaI GGTCTCn'nnnn_
Cuts at: 0 910 1002
Size: 910 92
BsaAI yAC'GTr
Cuts at: 0 928 1002
Size: 928 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 653 683 782 812 1002
Size: 653 30 99 30 190
Fragments arranged by size:
653 190 99 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 700 1002
Size: 359 341 302
Fragments arranged by size:
359 341 302
Bsp1286I G_dGCh'C
Cuts at: 0 183 265 1002
Size: 183 82 737
Fragments arranged by size:
737 183 82
BsrGI T'GTAC_A
Cuts at: 0 62 1002
Size: 62 940
BstXI CCAn_nnnn'nTGG
Cuts at: 0 258 1002
Size: 258 744
BstYI r'GATC_y
Cuts at: 0 79 939 1002
Size: 79 860 63
Fragments arranged by size:
860 79 63
BtgI C'CryG_G
Cuts at: 0 803 1002
Size: 803 199
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 189 1002
Size: 189 813
BtsI GCAGTG_nn'
Cuts at: 0 55 1002
Size: 55 947
EaeI y'GGCC_r
Cuts at: 0 248 1002
Size: 248 754
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 438 1002
Size: 50 388 564
Fragments arranged by size:
564 388 50
EcoRV GAT'ATC
Cuts at: 0 126 1002
Size: 126 876
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 468 1002
Size: 468 534
HaeII r_GCGC'y
Cuts at: 0 695 1002
Size: 695 307
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 558 590 653 685 784 811 816 843
Size: 558 32 63 32 99 27 5 27
Cuts at: 843 1002
Size: 159
Fragments arranged by size:
558 159 99 63 32 32 27 27
5
HincII GTy'rAC
Cuts at: 0 585 1002
Size: 585 417
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 1002
Size: 101 901
MscI TGG'CCA
Cuts at: 0 250 1002
Size: 250 752
MslI CAynn'nnrTG
Cuts at: 0 133 256 676 1002
Size: 133 123 420 326
Fragments arranged by size:
420 326 133 123
MspA1I CmG'CkG
Cuts at: 0 37 650 1002
Size: 37 613 352
Fragments arranged by size:
613 352 37
NcoI C'CATG_G
Cuts at: 0 803 1002
Size: 803 199
PasI CC'CwG_GG
Cuts at: 0 333 1002
Size: 333 669
PfoI T'CCnGG_A
Cuts at: 0 146 1002
Size: 146 856
PmlI CAC'GTG
Cuts at: 0 928 1002
Size: 928 74
PvuII CAG'CTG
Cuts at: 0 37 650 1002
Size: 37 613 352
Fragments arranged by size:
613 352 37
SmlI C'TyrA_G
Cuts at: 0 655 1002
Size: 655 347
StyI C'CwwG_G
Cuts at: 0 443 593 803 1002
Size: 443 150 210 199
Fragments arranged by size:
443 210 199 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 130 796 1002
Size: 130 666 206
Fragments arranged by size:
666 206 130
TatI w'GTAC_w
Cuts at: 0 62 1002
Size: 62 940
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 782 814 1002
Size: 782 32 188
Fragments arranged by size:
782 188 32
Tth111I GACn'n_nGTC
Cuts at: 0 154 757 1002
Size: 154 603 245
Fragments arranged by size:
603 245 154
XmnI GAAnn'nnTTC
Cuts at: 0 322 558 1002
Size: 322 236 444
Fragments arranged by size:
444 322 236
Enzymes that do cut:
AcuI AleI AlfI ApoI AseI BanII BbsI BciVI
Bme1580I BmgBI BpmI BpuEI BsaI BsaAI BsaXI BsgI
Bsp1286I BsrGI BstXI BstYI BtgI BtgZI BtsI EaeI
Eco57MI EcoRV FalI HaeII Hin4I HincII MmeI MscI
MslI MspA1I NcoI PasI PfoI PmlI PvuII SmlI
StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BclI BfrBI BglI BglII BlpI BmrI BmtI BplI
Bpu10I BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoNI EcoO109I EcoRI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XcmI XhoI XmaI ZraI