Restriction map for
pFN21AE5459
(Linear) (Six-base) MAPSORT of: KIEE5459.seq Check: 5819 from: 1 to: 945
KIEE5459.orf
With 182 enzymes: SgfI *
January 29, 2014 12:01 ..
Acc65I G'GTAC_C
Cuts at: 0 631 945
Size: 631 314
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 867 945
Size: 867 78
AflIII A'CryG_T
Cuts at: 0 727 945
Size: 727 218
AlwNI CAG_nnn'CTG
Cuts at: 0 13 945
Size: 13 932
ApoI r'AATT_y
Cuts at: 0 689 833 945
Size: 689 144 112
Fragments arranged by size:
689 144 112
BanI G'GyrC_C
Cuts at: 0 66 631 945
Size: 66 565 314
Fragments arranged by size:
565 314 66
BanII G_rGCy'C
Cuts at: 0 167 945
Size: 167 778
BbeI G_GCGC'C
Cuts at: 0 70 945
Size: 70 875
BbvCI CC'TCA_GC
Cuts at: 0 136 945
Size: 136 809
BclI T'GATC_A
Cuts at: 0 527 945
Size: 527 418
BmrI ACTGGGnnnn_n'
Cuts at: 0 802 945
Size: 802 143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 561 933 945
Size: 82 479 372 12
Fragments arranged by size:
479 372 82 12
Bpu10I CC'TnA_GC
Cuts at: 0 136 945
Size: 136 809
BsaAI yAC'GTr
Cuts at: 0 669 945
Size: 669 276
BsaHI Gr'CG_yC
Cuts at: 0 67 945
Size: 67 878
BseYI C'CCAG_C
Cuts at: 0 51 305 945
Size: 51 254 640
Fragments arranged by size:
640 254 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 919 945
Size: 299 620 26
Fragments arranged by size:
620 299 26
BsiHKAI G_wGCw'C
Cuts at: 0 98 945
Size: 98 847
BsmBI CGTCTCn'nnnn_
Cuts at: 0 265 945
Size: 265 680
Bsp1286I G_dGCh'C
Cuts at: 0 98 167 945
Size: 98 69 778
Fragments arranged by size:
778 98 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 619 945
Size: 619 326
BsrDI GCAATG_nn'
Cuts at: 0 32 945
Size: 32 913
BsrFI r'CCGG_y
Cuts at: 0 389 945
Size: 389 556
BtgI C'CryG_G
Cuts at: 0 359 638 945
Size: 359 279 307
Fragments arranged by size:
359 307 279
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 495 530 945
Size: 495 35 415
Fragments arranged by size:
495 415 35
DraIII CAC_nnn'GTG
Cuts at: 0 489 945
Size: 489 456
EaeI y'GGCC_r
Cuts at: 0 380 491 945
Size: 380 111 454
Fragments arranged by size:
454 380 111
EarI CTCTTCn'nnn_
Cuts at: 0 103 316 565 945
Size: 103 213 249 380
Fragments arranged by size:
380 249 213 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 561 867 933 945
Size: 82 479 306 66 12
Fragments arranged by size:
479 306 82 66 12
EcoO109I rG'GnC_Cy
Cuts at: 0 293 945
Size: 293 652
EcoRI G'AATT_C
Cuts at: 0 689 945
Size: 689 256
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 735 945
Size: 735 210
HaeII r_GCGC'y
Cuts at: 0 70 945
Size: 70 875
HindIII A'AGCT_T
Cuts at: 0 805 945
Size: 805 140
KasI G'GCGC_C
Cuts at: 0 66 945
Size: 66 879
KpnI G_GTAC'C
Cuts at: 0 635 945
Size: 635 310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 810 945
Size: 312 498 135
Fragments arranged by size:
498 312 135
MscI TGG'CCA
Cuts at: 0 382 493 945
Size: 382 111 452
Fragments arranged by size:
452 382 111
MslI CAynn'nnrTG
Cuts at: 0 181 412 945
Size: 181 231 533
Fragments arranged by size:
533 231 181
MspA1I CmG'CkG
Cuts at: 0 151 676 945
Size: 151 525 269
Fragments arranged by size:
525 269 151
NaeI GCC'GGC
Cuts at: 0 391 945
Size: 391 554
NarI GG'CG_CC
Cuts at: 0 67 945
Size: 67 878
NcoI C'CATG_G
Cuts at: 0 359 638 945
Size: 359 279 307
Fragments arranged by size:
359 307 279
NgoMIV G'CCGG_C
Cuts at: 0 389 945
Size: 389 556
NspI r_CATG'y
Cuts at: 0 731 945
Size: 731 214
PciI A'CATG_T
Cuts at: 0 727 945
Size: 727 218
PflMI CCAn_nnn'nTGG
Cuts at: 0 13 489 945
Size: 13 476 456
Fragments arranged by size:
476 456 13
PmlI CAC'GTG
Cuts at: 0 669 945
Size: 669 276
PsiI TTA'TAA
Cuts at: 0 622 945
Size: 622 323
PstI C_TGCA'G
Cuts at: 0 80 681 945
Size: 80 601 264
Fragments arranged by size:
601 264 80
PvuII CAG'CTG
Cuts at: 0 151 676 945
Size: 151 525 269
Fragments arranged by size:
525 269 151
SapI GCTCTTCn'nnn_
Cuts at: 0 103 316 565 945
Size: 103 213 249 380
Fragments arranged by size:
380 249 213 103
SexAI A'CCwGG_T
Cuts at: 0 113 945
Size: 113 832
SfcI C'TryA_G
Cuts at: 0 76 402 677 698 876 945
Size: 76 326 275 21 178 69
Fragments arranged by size:
326 275 178 76 69 21
SfoI GGC'GCC
Cuts at: 0 68 945
Size: 68 877
StyI C'CwwG_G
Cuts at: 0 359 638 945
Size: 359 279 307
Fragments arranged by size:
359 307 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 212 386 598 945
Size: 212 174 212 347
Fragments arranged by size:
347 212 212 174
TatI w'GTAC_w
Cuts at: 0 185 945
Size: 185 760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 208 240 545 577 945
Size: 208 32 305 32 368
Fragments arranged by size:
368 305 208 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 328 945
Size: 328 617
XmnI GAAnn'nnTTC
Cuts at: 0 718 945
Size: 718 227
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI ApoI BanI BanII BbeI
BbvCI BclI BmrI BpmI Bpu10I BsaAI BsaHI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BtgI
CspCI DraIII EaeI EarI Eco57MI EcoO109I EcoRI FalI
HaeII HindIII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmlI
PsiI PstI PvuII SapI SexAI SfcI SfoI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbsI BcgI BciVI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI BpuEI
BsaI BsaBI BsaWI BsaXI BseRI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
DraI DrdI EagI EciI EcoICRI EcoNI EcoRV FseI
FspI FspAI Hin4I HincII HpaI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmaI ZraI