Restriction map for
pFN21AE5456
(Linear) (Six-base) MAPSORT of: KIEE5456.seq Check: 2253 from: 1 to: 933
KIEE5456.orf
With 182 enzymes: SgfI *
January 29, 2014 12:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 933
Size: 697 236
AflII C'TTAA_G
Cuts at: 0 552 933
Size: 552 381
AleI CACnn'nnGTG
Cuts at: 0 22 319 838 933
Size: 22 297 519 95
Fragments arranged by size:
519 297 95 22
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 447 479 933
Size: 447 32 454
Fragments arranged by size:
454 447 32
ApoI r'AATT_y
Cuts at: 0 923 933
Size: 923 10
AvrII C'CTAG_G
Cuts at: 0 282 933
Size: 282 651
BamHI G'GATC_C
Cuts at: 0 494 933
Size: 494 439
BanII G_rGCy'C
Cuts at: 0 688 919 933
Size: 688 231 14
Fragments arranged by size:
688 231 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 327 741 933
Size: 327 414 192
Fragments arranged by size:
414 327 192
BbvCI CC'TCA_GC
Cuts at: 0 187 933
Size: 187 746
BclI T'GATC_A
Cuts at: 0 635 933
Size: 635 298
BglI GCCn_nnn'nGGC
Cuts at: 0 352 933
Size: 352 581
BglII A'GATC_T
Cuts at: 0 508 933
Size: 508 425
BlpI GC'TnA_GC
Cuts at: 0 483 933
Size: 483 450
BmrI ACTGGGnnnn_n'
Cuts at: 0 779 933
Size: 779 154
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 94 933
Size: 94 839
Bpu10I CC'TnA_GC
Cuts at: 0 187 933
Size: 187 746
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 210 933
Size: 210 723
Bsp1286I G_dGCh'C
Cuts at: 0 688 919 933
Size: 688 231 14
Fragments arranged by size:
688 231 14
BspHI T'CATG_A
Cuts at: 0 632 933
Size: 632 301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 662 933
Size: 662 271
BstYI r'GATC_y
Cuts at: 0 494 508 933
Size: 494 14 425
Fragments arranged by size:
494 425 14
BtgI C'CryG_G
Cuts at: 0 753 933
Size: 753 180
BtsI GCAGTG_nn'
Cuts at: 0 589 933
Size: 589 344
EaeI y'GGCC_r
Cuts at: 0 344 371 605 818 933
Size: 344 27 234 213 115
Fragments arranged by size:
344 234 213 115 27
EarI CTCTTCn'nnn_
Cuts at: 0 85 933
Size: 85 848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 933
Size: 697 236
EcoRV GAT'ATC
Cuts at: 0 573 933
Size: 573 360
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 578 933
Size: 578 355
MscI TGG'CCA
Cuts at: 0 373 607 820 933
Size: 373 234 213 113
Fragments arranged by size:
373 234 213 113
MslI CAynn'nnrTG
Cuts at: 0 22 172 319 838 933
Size: 22 150 147 519 95
Fragments arranged by size:
519 150 147 95 22
NcoI C'CATG_G
Cuts at: 0 753 933
Size: 753 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 410 933
Size: 410 523
PstI C_TGCA'G
Cuts at: 0 693 933
Size: 693 240
SfcI C'TryA_G
Cuts at: 0 203 504 689 867 933
Size: 203 301 185 178 66
Fragments arranged by size:
301 203 185 178 66
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 352 933
Size: 352 581
SmlI C'TyrA_G
Cuts at: 0 552 933
Size: 552 381
StyI C'CwwG_G
Cuts at: 0 42 282 753 933
Size: 42 240 471 180
Fragments arranged by size:
471 240 180 42
TatI w'GTAC_w
Cuts at: 0 176 933
Size: 176 757
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 405 437 581 613 933
Size: 405 32 144 32 320
Fragments arranged by size:
405 320 144 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 678 933
Size: 678 255
Enzymes that do cut:
AcuI AflII AleI AloI ApoI AvrII BamHI BanII
BbsI BbvCI BclI BglI BglII BlpI BmrI BplI
Bpu10I BseRI Bsp1286I BspHI BspMI BstYI BtgI BtsI
EaeI EarI Eco57MI EcoRV FalI MscI MslI NcoI
PflMI PstI SfcI SfiI SmlI StyI TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BanI BbeI BcgI BciVI BfrBI Bme1580I
BmgBI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XhoI
XmaI XmnI ZraI