Restriction map for
pFN21AE5453
(Linear) (Six-base) MAPSORT of: KIEE5453.seq Check: 4675 from: 1 to: 1056
KIEE5453.orf
With 182 enzymes: SgfI *
January 29, 2014 12:01 ..
Acc65I G'GTAC_C
Cuts at: 0 19 1056
Size: 19 1037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 572 1056
Size: 200 372 484
Fragments arranged by size:
484 372 200
AflIII A'CryG_T
Cuts at: 0 587 1056
Size: 587 469
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 869 901 1056
Size: 869 32 155
Fragments arranged by size:
869 155 32
AlwNI CAG_nnn'CTG
Cuts at: 0 735 1056
Size: 735 321
ApoI r'AATT_y
Cuts at: 0 618 1056
Size: 618 438
AvaI C'yCGr_G
Cuts at: 0 90 389 1056
Size: 90 299 667
Fragments arranged by size:
667 299 90
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 811 844 1056
Size: 811 33 212
Fragments arranged by size:
811 212 33
BanI G'GyrC_C
Cuts at: 0 19 1056
Size: 19 1037
BbvCI CC'TCA_GC
Cuts at: 0 254 1056
Size: 254 802
BclI T'GATC_A
Cuts at: 0 336 629 1056
Size: 336 293 427
Fragments arranged by size:
427 336 293
BlpI GC'TnA_GC
Cuts at: 0 691 1056
Size: 691 365
Bme1580I G_kGCm'C
Cuts at: 0 396 1056
Size: 396 660
Bpu10I CC'TnA_GC
Cuts at: 0 254 1056
Size: 254 802
BsaAI yAC'GTr
Cuts at: 0 348 1056
Size: 348 708
BsaHI Gr'CG_yC
Cuts at: 0 4 1056
Size: 4 1052
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 869 886 899 916 1056
Size: 869 17 13 17 140
Fragments arranged by size:
869 140 17 17 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 887 907 1056
Size: 887 20 149
Fragments arranged by size:
887 149 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 1056
Size: 136 920
Bsp1286I G_dGCh'C
Cuts at: 0 396 1056
Size: 396 660
BsrBI CCG'CTC
Cuts at: 0 885 1056
Size: 885 171
BsrDI GCAATG_nn'
Cuts at: 0 953 1056
Size: 953 103
BsrFI r'CCGG_y
Cuts at: 0 22 55 1056
Size: 22 33 1001
Fragments arranged by size:
1001 33 22
BstYI r'GATC_y
Cuts at: 0 478 1056
Size: 478 578
Bsu36I CC'TnA_GG
Cuts at: 0 890 1056
Size: 890 166
BtsI GCAGTG_nn'
Cuts at: 0 998 1056
Size: 998 58
EarI CTCTTCn'nnn_
Cuts at: 0 45 916 1027 1043 1056
Size: 45 871 111 16 13
Fragments arranged by size:
871 111 45 16 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 572 1056
Size: 200 372 484
Fragments arranged by size:
484 372 200
EcoO109I rG'GnC_Cy
Cuts at: 0 897 1056
Size: 897 159
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 214 241 1056
Size: 214 27 815
Fragments arranged by size:
815 214 27
HaeII r_GCGC'y
Cuts at: 0 864 1056
Size: 864 192
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 886 918 1056
Size: 886 32 138
Fragments arranged by size:
886 138 32
KpnI G_GTAC'C
Cuts at: 0 23 1056
Size: 23 1033
MslI CAynn'nnrTG
Cuts at: 0 787 1056
Size: 787 269
MspA1I CmG'CkG
Cuts at: 0 667 1056
Size: 667 389
NaeI GCC'GGC
Cuts at: 0 57 1056
Size: 57 999
NgoMIV G'CCGG_C
Cuts at: 0 55 1056
Size: 55 1001
NspI r_CATG'y
Cuts at: 0 427 591 1056
Size: 427 164 465
Fragments arranged by size:
465 427 164
PciI A'CATG_T
Cuts at: 0 587 1056
Size: 587 469
PfoI T'CCnGG_A
Cuts at: 0 398 1056
Size: 398 658
PmlI CAC'GTG
Cuts at: 0 348 1056
Size: 348 708
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 869 901 1056
Size: 869 32 155
Fragments arranged by size:
869 155 32
PpuMI rG'GwC_Cy
Cuts at: 0 897 1056
Size: 897 159
PvuII CAG'CTG
Cuts at: 0 667 1056
Size: 667 389
SapI GCTCTTCn'nnn_
Cuts at: 0 1027 1043 1056
Size: 1027 16 13
Fragments arranged by size:
1027 16 13
SfcI C'TryA_G
Cuts at: 0 727 1056
Size: 727 329
SgrAI Cr'CCGG_yG
Cuts at: 0 55 1056
Size: 55 1001
SmaI CCC'GGG
Cuts at: 0 391 1056
Size: 391 665
SpeI A'CTAG_T
Cuts at: 0 799 1056
Size: 799 257
SphI G_CATG'C
Cuts at: 0 427 1056
Size: 427 629
StyI C'CwwG_G
Cuts at: 0 474 1056
Size: 474 582
TatI w'GTAC_w
Cuts at: 0 584 1056
Size: 584 472
XmaI C'CCGG_G
Cuts at: 0 389 1056
Size: 389 667
XmnI GAAnn'nnTTC
Cuts at: 0 185 1056
Size: 185 871
Enzymes that do cut:
Acc65I AcuI AflIII AloI AlwNI ApoI AvaI BaeI
BanI BbvCI BclI BlpI Bme1580I Bpu10I BsaAI BsaHI
BsaXI BseRI BsgI Bsp1286I BsrBI BsrDI BsrFI BstYI
Bsu36I BtsI EarI Eco57MI EcoO109I FalI HaeII Hin4I
KpnI MslI MspA1I NaeI NgoMIV NspI PciI PfoI
PmlI PpiI PpuMI PvuII SapI SfcI SgrAI SmaI
SpeI SphI StyI TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BanII BbeI BbsI BcgI BciVI BfrBI BglI
BglII BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaBI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
MfeI MluI MmeI MscI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmeI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SmlI SnaBI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI ZraI