Restriction map for
pFN21AE5452
(Linear) (Six-base) MAPSORT of: KIEE5452.seq Check: 3948 from: 1 to: 1053
KIEE5452.orf
With 182 enzymes: SgfI *
January 29, 2014 12:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 899 1053
Size: 661 238 154
Fragments arranged by size:
661 238 154
AgeI A'CCGG_T
Cuts at: 0 260 1053
Size: 260 793
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 482 1053
Size: 482 571
AlwNI CAG_nnn'CTG
Cuts at: 0 120 749 1053
Size: 120 629 304
Fragments arranged by size:
629 304 120
ApaI G_GGCC'C
Cuts at: 0 29 1053
Size: 29 1024
ApaLI G'TGCA_C
Cuts at: 0 172 1053
Size: 172 881
AseI AT'TA_AT
Cuts at: 0 434 1053
Size: 434 619
AvaI C'yCGr_G
Cuts at: 0 213 1053
Size: 213 840
BanI G'GyrC_C
Cuts at: 0 944 1053
Size: 944 109
BanII G_rGCy'C
Cuts at: 0 29 1053
Size: 29 1024
BbsI GAAGACnn'nnnn_
Cuts at: 0 234 1053
Size: 234 819
BbvCI CC'TCA_GC
Cuts at: 0 625 1053
Size: 625 428
BfrBI ATG'CAT
Cuts at: 0 401 1053
Size: 401 652
BglI GCCn_nnn'nGGC
Cuts at: 0 47 1053
Size: 47 1006
Bme1580I G_kGCm'C
Cuts at: 0 29 176 1053
Size: 29 147 877
Fragments arranged by size:
877 147 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 271 377 465 1053
Size: 207 64 106 88 588
Fragments arranged by size:
588 207 106 88 64
Bpu10I CC'TnA_GC
Cuts at: 0 625 1053
Size: 625 428
BsaAI yAC'GTr
Cuts at: 0 102 1053
Size: 102 951
BsaHI Gr'CG_yC
Cuts at: 0 201 352 844 1053
Size: 201 151 492 209
Fragments arranged by size:
492 209 201 151
BsaWI w'CCGG_w
Cuts at: 0 260 1053
Size: 260 793
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 547 577 898 928 1053
Size: 547 30 321 30 125
Fragments arranged by size:
547 321 125 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 749 1053
Size: 670 79 304
Fragments arranged by size:
670 304 79
BsiHKAI G_wGCw'C
Cuts at: 0 76 176 1053
Size: 76 100 877
Fragments arranged by size:
877 100 76
Bsp1286I G_dGCh'C
Cuts at: 0 29 76 176 1053
Size: 29 47 100 877
Fragments arranged by size:
877 100 47 29
BsrBI CCG'CTC
Cuts at: 0 47 1053
Size: 47 1006
BsrDI GCAATG_nn'
Cuts at: 0 401 464 612 872 1053
Size: 401 63 148 260 181
Fragments arranged by size:
401 260 181 148 63
BsrFI r'CCGG_y
Cuts at: 0 260 1053
Size: 260 793
BstEII G'GTnAC_C
Cuts at: 0 492 1053
Size: 492 561
BtgI C'CryG_G
Cuts at: 0 20 67 701 1053
Size: 20 47 634 352
Fragments arranged by size:
634 352 47 20
DraIII CAC_nnn'GTG
Cuts at: 0 180 1053
Size: 180 873
DrdI GACnn_nn'nnGTC
Cuts at: 0 265 1053
Size: 265 788
EaeI y'GGCC_r
Cuts at: 0 233 1053
Size: 233 820
EarI CTCTTCn'nnn_
Cuts at: 0 527 1053
Size: 527 526
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 271 377 465 661 899 1053
Size: 207 64 106 88 196 238 154
Fragments arranged by size:
238 207 196 154 106 88 64
EcoO109I rG'GnC_Cy
Cuts at: 0 25 26 218 1053
Size: 25 1 192 835
Fragments arranged by size:
835 192 25 1
HaeII r_GCGC'y
Cuts at: 0 167 1053
Size: 167 886
HincII GTy'rAC
Cuts at: 0 555 1053
Size: 555 498
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 949 1053
Size: 698 251 104
Fragments arranged by size:
698 251 104
MspA1I CmG'CkG
Cuts at: 0 13 86 1053
Size: 13 73 967
Fragments arranged by size:
967 73 13
NcoI C'CATG_G
Cuts at: 0 20 701 1053
Size: 20 681 352
Fragments arranged by size:
681 352 20
NsiI A_TGCA'T
Cuts at: 0 403 1053
Size: 403 650
PfoI T'CCnGG_A
Cuts at: 0 565 1053
Size: 565 488
PpuMI rG'GwC_Cy
Cuts at: 0 218 1053
Size: 218 835
PshAI GACnn'nnGTC
Cuts at: 0 200 1053
Size: 200 853
PspOMI G'GGCC_C
Cuts at: 0 25 1053
Size: 25 1028
PstI C_TGCA'G
Cuts at: 0 18 1053
Size: 18 1035
SapI GCTCTTCn'nnn_
Cuts at: 0 527 1053
Size: 527 526
ScaI AGT'ACT
Cuts at: 0 712 1053
Size: 712 341
SfcI C'TryA_G
Cuts at: 0 14 470 1053
Size: 14 456 583
Fragments arranged by size:
583 456 14
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 47 1053
Size: 47 1006
StuI AGG'CCT
Cuts at: 0 957 1053
Size: 957 96
StyI C'CwwG_G
Cuts at: 0 20 167 701 1053
Size: 20 147 534 352
Fragments arranged by size:
534 352 147 20
TatI w'GTAC_w
Cuts at: 0 710 1053
Size: 710 343
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 898 930 1053
Size: 898 32 123
Fragments arranged by size:
898 123 32
Enzymes that do cut:
AcuI AgeI AlfI AlwNI ApaI ApaLI AseI AvaI
BanI BanII BbsI BbvCI BfrBI BglI Bme1580I BpmI
Bpu10I BsaAI BsaHI BsaWI BsaXI BsgI BsiHKAI Bsp1286I
BsrBI BsrDI BsrFI BstEII BtgI DraIII DrdI EaeI
EarI Eco57MI EcoO109I HaeII HincII MmeI MspA1I NcoI
NsiI PfoI PpuMI PshAI PspOMI PstI SapI ScaI
SfcI SfiI StuI StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AloI ApoI AscI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BclI BglII BlpI BmgBI
BmrI BmtI BplI BpuEI BsaI BsaBI BseRI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
NaeI NarI NdeI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI SexAI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI