(Linear) (Six-base) MAPSORT of: KIEE5451.seq  Check: 3873  from: 1  to: 1050



KIEE5451.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:01  ..

AatII G_ACGT'C

Cuts at:      0     202    1050 
   Size:        202     848
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     753     973    1049    1050 
   Size:        753     220      76       1

  Fragments arranged by size: 

                 753     220      76       1
 
AlwNI CAG_nnn'CTG

Cuts at:      0     307     850    1050 
   Size:        307     543     200

  Fragments arranged by size: 

                 543     307     200
 
ApaI G_GGCC'C

Cuts at:      0     499    1050 
   Size:        499     551
 
ApoI r'AATT_y

Cuts at:      0     177     688    1050 
   Size:        177     511     362

  Fragments arranged by size: 

                 511     362     177
 
BamHI G'GATC_C

Cuts at:      0     651    1050 
   Size:        651     399
 
BanII G_rGCy'C

Cuts at:      0     499    1050 
   Size:        499     551
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     441    1050 
   Size:        441     609
 
BfrBI ATG'CAT

Cuts at:      0     564     832    1050 
   Size:        564     268     218

  Fragments arranged by size: 

                 564     268     218
 
Bme1580I G_kGCm'C

Cuts at:      0     499    1050 
   Size:        499     551
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     926    1035    1050 
   Size:        926     109      15

  Fragments arranged by size: 

                 926     109      15
 
BsaHI Gr'CG_yC

Cuts at:      0       9     199    1050 
   Size:          9     190     851

  Fragments arranged by size: 

                 851     190       9
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     206    1050 
   Size:        206     844
 
Bsp1286I G_dGCh'C

Cuts at:      0     499    1050 
   Size:        499     551
 
BstYI r'GATC_y

Cuts at:      0     333     513     651    1050 
   Size:        333     180     138     399

  Fragments arranged by size: 

                 399     333     180     138
 
BtgI C'CryG_G

Cuts at:      0      32      87    1050 
   Size:         32      55     963

  Fragments arranged by size: 

                 963      55      32
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     504    1050 
   Size:        504     546
 
BtsI GCAGTG_nn'

Cuts at:      0     296     552    1050 
   Size:        296     256     498

  Fragments arranged by size: 

                 498     296     256
 
DraIII CAC_nnn'GTG

Cuts at:      0     786    1050 
   Size:        786     264
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      72    1050 
   Size:         72     978
 
EarI CTCTTCn'nnn_

Cuts at:      0     730     772    1050 
   Size:        730      42     278

  Fragments arranged by size: 

                 730     278      42
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     753     926     973    1035    1049    1050 
   Size:        753     173      47      62      14       1

  Fragments arranged by size: 

                 753     173      62      47      14       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      19     209    1050 
   Size:         19     190     841

  Fragments arranged by size: 

                 841     190      19
 
EcoO109I rG'GnC_Cy

Cuts at:      0      74     495    1050 
   Size:         74     421     555

  Fragments arranged by size: 

                 555     421      74
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     603    1050 
   Size:        603     447
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     636     668    1050 
   Size:        636      32     382

  Fragments arranged by size: 

                 636     382      32
 
HindIII A'AGCT_T

Cuts at:      0     955    1050 
   Size:        955      95
 
MspA1I CmG'CkG

Cuts at:      0      34      89    1050 
   Size:         34      55     961

  Fragments arranged by size: 

                 961      55      34
 
NsiI A_TGCA'T

Cuts at:      0     566     834    1050 
   Size:        566     268     216

  Fragments arranged by size: 

                 566     268     216
 
NspI r_CATG'y

Cuts at:      0     391    1050 
   Size:        391     659
 
PasI CC'CwG_GG

Cuts at:      0     637    1050 
   Size:        637     413
 
PfoI T'CCnGG_A

Cuts at:      0     654    1050 
   Size:        654     396
 
PpuMI rG'GwC_Cy

Cuts at:      0      74    1050 
   Size:         74     976
 
PsiI TTA'TAA

Cuts at:      0     523    1050 
   Size:        523     527
 
PspOMI G'GGCC_C

Cuts at:      0     495    1050 
   Size:        495     555
 
PstI C_TGCA'G

Cuts at:      0     303     453     513    1050 
   Size:        303     150      60     537

  Fragments arranged by size: 

                 537     303     150      60
 
SacII CC_GC'GG

Cuts at:      0      35      90    1050 
   Size:         35      55     960

  Fragments arranged by size: 

                 960      55      35
 
SbfI CC_TGCA'GG

Cuts at:      0     453    1050 
   Size:        453     597
 
SfcI C'TryA_G

Cuts at:      0     299     449     509    1050 
   Size:        299     150      60     541

  Fragments arranged by size: 

                 541     299     150      60
 
StyI C'CwwG_G

Cuts at:      0      53    1050 
   Size:         53     997
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     453     921    1050 
   Size:        453     468     129

  Fragments arranged by size: 

                 468     453     129
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     603     635    1050 
   Size:        603      32     415

  Fragments arranged by size: 

                 603     415      32
 
ZraI GAC'GTC

Cuts at:      0     200    1050 
   Size:        200     850
 

 Enzymes that do cut:

    AatII     AcuI    AlwNI     ApaI     ApoI    BamHI    BanII     BbsI
    BfrBI Bme1580I     BpmI    BsaHI    BsmBI Bsp1286I    BstYI     BtgI
    BtgZI     BtsI   DraIII     DrdI     EarI  Eco57MI    EcoNI EcoO109I
     FalI    Hin4I  HindIII   MspA1I     NsiI     NspI     PasI     PfoI
    PpuMI     PsiI   PspOMI     PstI    SacII     SbfI     SfcI     StyI
    TaqII     TstI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI
     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI