(Linear) (Six-base) MAPSORT of: KIEE5447.seq  Check: 6570  from: 1  to: 984



KIEE5447.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     943     984 
   Size:        943      41
 
AccI GT'mk_AC

Cuts at:      0     398     984 
   Size:        398     586
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     754     984 
   Size:        754     230
 
AleI CACnn'nnGTG

Cuts at:      0      81     984 
   Size:         81     903
 
AlwNI CAG_nnn'CTG

Cuts at:      0     549     984 
   Size:        549     435
 
ApoI r'AATT_y

Cuts at:      0     700     984 
   Size:        700     284
 
AseI AT'TA_AT

Cuts at:      0     713     984 
   Size:        713     271
 
BanI G'GyrC_C

Cuts at:      0     955     984 
   Size:        955      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      44     984 
   Size:         44     940
 
BbvCI CC'TCA_GC

Cuts at:      0     857     984 
   Size:        857     127
 
BclI T'GATC_A

Cuts at:      0     407     984 
   Size:        407     577
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25      34     356     484     847     984 
   Size:         25       9     322     128     363     137

  Fragments arranged by size: 

                 363     322     137     128      25       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     857     984 
   Size:        857     127
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     475     984 
   Size:        475     509
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     823     984 
   Size:        823     161
 
BsmI GAATG_Cn'

Cuts at:      0     230     462     984 
   Size:        230     232     522

  Fragments arranged by size: 

                 522     232     230
 
BspHI T'CATG_A

Cuts at:      0      68     536     984 
   Size:         68     468     448

  Fragments arranged by size: 

                 468     448      68
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     943     984 
   Size:        943      41
 
BsrDI GCAATG_nn'

Cuts at:      0     581     984 
   Size:        581     403
 
BstEII G'GTnAC_C

Cuts at:      0     756     984 
   Size:        756     228
 
BstZ17I GTA'TAC

Cuts at:      0     399     984 
   Size:        399     585
 
EarI CTCTTCn'nnn_

Cuts at:      0     391     984 
   Size:        391     593
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25      34     356     484     754     847     984 
   Size:         25       9     322     128     270      93     137

  Fragments arranged by size: 

                 322     270     137     128      93      25       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     765     984 
   Size:        765     219
 
EcoRV GAT'ATC

Cuts at:      0     750     984 
   Size:        750     234
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     171     549     984 
   Size:        171     378     435

  Fragments arranged by size: 

                 435     378     171
 
HaeII r_GCGC'y

Cuts at:      0     190     984 
   Size:        190     794
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     845     877     984 
   Size:        845      32     107

  Fragments arranged by size: 

                 845     107      32
 
HindIII A'AGCT_T

Cuts at:      0     112     984 
   Size:        112     872
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     899     984 
   Size:        124     775      85

  Fragments arranged by size: 

                 775     124      85
 
MslI CAynn'nnrTG

Cuts at:      0      81     631     984 
   Size:         81     550     353

  Fragments arranged by size: 

                 550     353      81
 
NspI r_CATG'y

Cuts at:      0      63     984 
   Size:         63     921
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     870     984 
   Size:        870     114
 
SmlI C'TyrA_G

Cuts at:      0     490     984 
   Size:        490     494
 
StuI AGG'CCT

Cuts at:      0     151     984 
   Size:        151     833
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI    AlwNI     ApoI     AseI     BanI
     BbsI    BbvCI     BclI     BpmI   Bpu10I    BpuEI     BsaI     BsmI
    BspHI    BspMI    BsrDI   BstEII  BstZ17I     EarI  Eco57MI EcoO109I
    EcoRV     FalI    HaeII    Hin4I  HindIII     MmeI     MslI     NspI
    PflMI     SmlI     StuI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BanII     BbeI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI     FseI     FspI    FspAI
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI