Restriction map for
pFN21AE5446
(Linear) (Six-base) MAPSORT of: KIEE5446.seq Check: 6667 from: 1 to: 966
KIEE5446.orf
With 182 enzymes: SgfI *
January 29, 2014 12:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 184 966
Size: 184 782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 579 966
Size: 555 24 387
Fragments arranged by size:
555 387 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 454 966
Size: 454 512
AlwNI CAG_nnn'CTG
Cuts at: 0 26 275 498 966
Size: 26 249 223 468
Fragments arranged by size:
468 249 223 26
ApoI r'AATT_y
Cuts at: 0 892 966
Size: 892 74
AvaI C'yCGr_G
Cuts at: 0 880 966
Size: 880 86
BamHI G'GATC_C
Cuts at: 0 757 966
Size: 757 209
BanI G'GyrC_C
Cuts at: 0 82 342 966
Size: 82 260 624
Fragments arranged by size:
624 260 82
BanII G_rGCy'C
Cuts at: 0 17 695 778 936 966
Size: 17 678 83 158 30
Fragments arranged by size:
678 158 83 30 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 360 369 561 966
Size: 360 9 192 405
Fragments arranged by size:
405 360 192 9
BfrBI ATG'CAT
Cuts at: 0 807 966
Size: 807 159
BglI GCCn_nnn'nGGC
Cuts at: 0 451 966
Size: 451 515
Bme1580I G_kGCm'C
Cuts at: 0 433 447 966
Size: 433 14 519
Fragments arranged by size:
519 433 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 285 966
Size: 285 681
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 609 797 966
Size: 184 425 188 169
Fragments arranged by size:
425 188 184 169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 966
Size: 689 277
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 139 966
Size: 109 30 827
Fragments arranged by size:
827 109 30
BseYI C'CCAG_C
Cuts at: 0 10 26 268 346 945 966
Size: 10 16 242 78 599 21
Fragments arranged by size:
599 242 78 21 16 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 941 966
Size: 229 712 25
Fragments arranged by size:
712 229 25
BsiHKAI G_wGCw'C
Cuts at: 0 778 966
Size: 778 188
BsmI GAATG_Cn'
Cuts at: 0 807 809 966
Size: 807 2 157
Fragments arranged by size:
807 157 2
Bsp1286I G_dGCh'C
Cuts at: 0 17 433 447 695 778 936 966
Size: 17 416 14 248 83 158 30
Fragments arranged by size:
416 248 158 83 30 17 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 80 184 201 349 966
Size: 80 104 17 148 617
Fragments arranged by size:
617 148 104 80 17
BstAPI GCAn_nnn'nTGC
Cuts at: 0 207 966
Size: 207 759
BstYI r'GATC_y
Cuts at: 0 749 757 966
Size: 749 8 209
Fragments arranged by size:
749 209 8
BtgI C'CryG_G
Cuts at: 0 194 563 726 966
Size: 194 369 163 240
Fragments arranged by size:
369 240 194 163
EaeI y'GGCC_r
Cuts at: 0 33 452 660 966
Size: 33 419 208 306
Fragments arranged by size:
419 306 208 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 130 966
Size: 130 836
EcoICRI GAG'CTC
Cuts at: 0 776 966
Size: 776 190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 555 579 609 797 966
Size: 184 371 24 30 188 169
Fragments arranged by size:
371 188 184 169 30 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 797 966
Size: 797 169
EcoO109I rG'GnC_Cy
Cuts at: 0 884 966
Size: 884 82
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 174 966
Size: 174 792
HaeII r_GCGC'y
Cuts at: 0 489 966
Size: 489 477
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 966
Size: 109 32 825
Fragments arranged by size:
825 109 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 944 966
Size: 125 819 22
Fragments arranged by size:
819 125 22
MscI TGG'CCA
Cuts at: 0 662 966
Size: 662 304
MspA1I CmG'CkG
Cuts at: 0 23 99 966
Size: 23 76 867
Fragments arranged by size:
867 76 23
NcoI C'CATG_G
Cuts at: 0 194 563 726 966
Size: 194 369 163 240
Fragments arranged by size:
369 240 194 163
NsiI A_TGCA'T
Cuts at: 0 809 966
Size: 809 157
PasI CC'CwG_GG
Cuts at: 0 531 966
Size: 531 435
PflMI CCAn_nnn'nTGG
Cuts at: 0 275 966
Size: 275 691
PfoI T'CCnGG_A
Cuts at: 0 161 966
Size: 161 805
PshAI GACnn'nnGTC
Cuts at: 0 541 966
Size: 541 425
PstI C_TGCA'G
Cuts at: 0 22 966
Size: 22 944
PvuII CAG'CTG
Cuts at: 0 23 99 966
Size: 23 76 867
Fragments arranged by size:
867 76 23
SacI G_AGCT'C
Cuts at: 0 778 966
Size: 778 188
SfcI C'TryA_G
Cuts at: 0 18 966
Size: 18 948
SmaI CCC'GGG
Cuts at: 0 882 966
Size: 882 84
SmlI C'TyrA_G
Cuts at: 0 704 966
Size: 704 262
StyI C'CwwG_G
Cuts at: 0 194 563 726 966
Size: 194 369 163 240
Fragments arranged by size:
369 240 194 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 219 360 576 966
Size: 219 141 216 390
Fragments arranged by size:
390 219 216 141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 724 966
Size: 724 242
XmaI C'CCGG_G
Cuts at: 0 880 966
Size: 880 86
XmnI GAAnn'nnTTC
Cuts at: 0 807 966
Size: 807 159
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApoI AvaI BamHI BanI
BanII BbsI BfrBI BglI Bme1580I BmrI BpmI BpuEI
BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BstAPI
BstYI BtgI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I MmeI MscI MspA1I NcoI NsiI
PasI PflMI PfoI PshAI PstI PvuII SacI SfcI
SmaI SmlI StyI TaqII XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BciVI BclI BglII
BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NspI PacI PciI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XhoI ZraI