Restriction map for
pFN21AE5445
(Linear) (Six-base) MAPSORT of: KIEE5445.seq Check: 695 from: 1 to: 948
KIEE5445.orf
With 182 enzymes: SgfI *
January 29, 2014 12:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 948
Size: 72 876
AflIII A'CryG_T
Cuts at: 0 529 948
Size: 529 419
ApaLI G'TGCA_C
Cuts at: 0 422 948
Size: 422 526
ApoI r'AATT_y
Cuts at: 0 536 948
Size: 536 412
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 286 319 948
Size: 286 33 629
Fragments arranged by size:
629 286 33
BanI G'GyrC_C
Cuts at: 0 721 948
Size: 721 227
BanII G_rGCy'C
Cuts at: 0 325 948
Size: 325 623
BbsI GAAGACnn'nnnn_
Cuts at: 0 78 948
Size: 78 870
BbvCI CC'TCA_GC
Cuts at: 0 684 948
Size: 684 264
BclI T'GATC_A
Cuts at: 0 140 948
Size: 140 808
BglI GCCn_nnn'nGGC
Cuts at: 0 352 948
Size: 352 596
Bme1580I G_kGCm'C
Cuts at: 0 426 726 948
Size: 426 300 222
Fragments arranged by size:
426 300 222
BmrI ACTGGGnnnn_n'
Cuts at: 0 48 948
Size: 48 900
Bpu10I CC'TnA_GC
Cuts at: 0 684 948
Size: 684 264
BsaI GGTCTCn'nnnn_
Cuts at: 0 444 948
Size: 444 504
BsaAI yAC'GTr
Cuts at: 0 530 948
Size: 530 418
BsaBI GATnn'nnATC
Cuts at: 0 145 948
Size: 145 803
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 601 948
Size: 601 347
BseYI C'CCAG_C
Cuts at: 0 909 948
Size: 909 39
BsiHKAI G_wGCw'C
Cuts at: 0 341 426 948
Size: 341 85 522
Fragments arranged by size:
522 341 85
BsmI GAATG_Cn'
Cuts at: 0 605 948
Size: 605 343
Bsp1286I G_dGCh'C
Cuts at: 0 325 341 426 726 948
Size: 325 16 85 300 222
Fragments arranged by size:
325 300 222 85 16
BspHI T'CATG_A
Cuts at: 0 404 578 948
Size: 404 174 370
Fragments arranged by size:
404 370 174
BsrBI CCG'CTC
Cuts at: 0 466 948
Size: 466 482
BsrDI GCAATG_nn'
Cuts at: 0 922 948
Size: 922 26
BsrGI T'GTAC_A
Cuts at: 0 294 948
Size: 294 654
BstXI CCAn_nnnn'nTGG
Cuts at: 0 594 948
Size: 594 354
BtgI C'CryG_G
Cuts at: 0 317 948
Size: 317 631
BtsI GCAGTG_nn'
Cuts at: 0 571 948
Size: 571 377
ClaI AT'CG_AT
Cuts at: 0 518 948
Size: 518 430
DraIII CAC_nnn'GTG
Cuts at: 0 332 948
Size: 332 616
EaeI y'GGCC_r
Cuts at: 0 344 371 948
Size: 344 27 577
Fragments arranged by size:
577 344 27
EarI CTCTTCn'nnn_
Cuts at: 0 85 948
Size: 85 863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 948
Size: 72 876
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 948
Size: 383 32 533
Fragments arranged by size:
533 383 32
MscI TGG'CCA
Cuts at: 0 373 948
Size: 373 575
MslI CAynn'nnrTG
Cuts at: 0 403 589 595 948
Size: 403 186 6 353
Fragments arranged by size:
403 353 186 6
MspA1I CmG'CkG
Cuts at: 0 387 948
Size: 387 561
NcoI C'CATG_G
Cuts at: 0 317 948
Size: 317 631
NdeI CA'TA_TG
Cuts at: 0 357 948
Size: 357 591
PasI CC'CwG_GG
Cuts at: 0 61 948
Size: 61 887
PmlI CAC'GTG
Cuts at: 0 530 948
Size: 530 418
PstI C_TGCA'G
Cuts at: 0 494 948
Size: 494 454
SfcI C'TryA_G
Cuts at: 0 490 948
Size: 490 458
StyI C'CwwG_G
Cuts at: 0 186 317 948
Size: 186 131 631
Fragments arranged by size:
631 186 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 275 435 948
Size: 275 160 513
Fragments arranged by size:
513 275 160
TatI w'GTAC_w
Cuts at: 0 176 294 948
Size: 176 118 654
Fragments arranged by size:
654 176 118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 594 948
Size: 594 354
XmnI GAAnn'nnTTC
Cuts at: 0 74 948
Size: 74 874
Enzymes that do cut:
AcuI AflIII ApaLI ApoI BaeI BanI BanII BbsI
BbvCI BclI BglI Bme1580I BmrI Bpu10I BsaI BsaAI
BsaBI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BsrBI
BsrDI BsrGI BstXI BtgI BtsI ClaI DraIII EaeI
EarI Eco57MI Hin4I MscI MslI MspA1I NcoI NdeI
PasI PmlI PstI SfcI StyI TaqII TatI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvaI AvrII BamHI BbeI BcgI BciVI BfrBI
BglII BlpI BmgBI BmtI BplI BpmI BpuEI BsaHI
BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspMI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI CspCI DraI DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmaI ZraI