Restriction map for
pFN21AE5443
(Linear) (Six-base) MAPSORT of: KIEE5443.seq Check: 6546 from: 1 to: 939
KIEE5443.orf
With 182 enzymes: SgfI *
January 29, 2014 12:00 ..
AccI GT'mk_AC
Cuts at: 0 478 699 939
Size: 478 221 240
Fragments arranged by size:
478 240 221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 939
Size: 713 226
AflIII A'CryG_T
Cuts at: 0 223 718 939
Size: 223 495 221
Fragments arranged by size:
495 223 221
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 708 740 939
Size: 708 32 199
Fragments arranged by size:
708 199 32
ApoI r'AATT_y
Cuts at: 0 249 939
Size: 249 690
BanII G_rGCy'C
Cuts at: 0 614 939
Size: 614 325
BbsI GAAGACnn'nnnn_
Cuts at: 0 81 915 939
Size: 81 834 24
Fragments arranged by size:
834 81 24
BciVI GTATCCnnnnn_n'
Cuts at: 0 674 939
Size: 674 265
BglI GCCn_nnn'nGGC
Cuts at: 0 322 939
Size: 322 617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 409 475 939
Size: 88 321 66 464
Fragments arranged by size:
464 321 88 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 939
Size: 538 401
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 407 437 939
Size: 407 30 502
Fragments arranged by size:
502 407 30
BseYI C'CCAG_C
Cuts at: 0 42 296 793 939
Size: 42 254 497 146
Fragments arranged by size:
497 254 146 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 939
Size: 530 409
BsiHKAI G_wGCw'C
Cuts at: 0 614 939
Size: 614 325
BsmBI CGTCTCn'nnnn_
Cuts at: 0 771 939
Size: 771 168
Bsp1286I G_dGCh'C
Cuts at: 0 614 939
Size: 614 325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 785 939
Size: 785 154
BsrDI GCAATG_nn'
Cuts at: 0 839 939
Size: 839 100
BsrGI T'GTAC_A
Cuts at: 0 226 939
Size: 226 713
BstYI r'GATC_y
Cuts at: 0 207 939
Size: 207 732
BtsI GCAGTG_nn'
Cuts at: 0 547 939
Size: 547 392
DraI TTT'AAA
Cuts at: 0 903 939
Size: 903 36
DraIII CAC_nnn'GTG
Cuts at: 0 505 939
Size: 505 434
EaeI y'GGCC_r
Cuts at: 0 344 939
Size: 344 595
EarI CTCTTCn'nnn_
Cuts at: 0 619 832 939
Size: 619 213 107
Fragments arranged by size:
619 213 107
EcoICRI GAG'CTC
Cuts at: 0 612 939
Size: 612 327
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 409 475 713 939
Size: 88 321 66 238 226
Fragments arranged by size:
321 238 226 88 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 360 939
Size: 360 579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 915 939
Size: 915 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 407 439 807 839 939
Size: 407 32 368 32 100
Fragments arranged by size:
407 368 100 32 32
HincII GTy'rAC
Cuts at: 0 700 939
Size: 700 239
MspA1I CmG'CkG
Cuts at: 0 436 495 939
Size: 436 59 444
Fragments arranged by size:
444 436 59
NdeI CA'TA_TG
Cuts at: 0 651 939
Size: 651 288
NspI r_CATG'y
Cuts at: 0 227 722 939
Size: 227 495 217
Fragments arranged by size:
495 227 217
PciI A'CATG_T
Cuts at: 0 223 718 939
Size: 223 495 221
Fragments arranged by size:
495 223 221
PfoI T'CCnGG_A
Cuts at: 0 203 939
Size: 203 736
PvuII CAG'CTG
Cuts at: 0 436 495 939
Size: 436 59 444
Fragments arranged by size:
444 436 59
SacI G_AGCT'C
Cuts at: 0 614 939
Size: 614 325
SalI G'TCGA_C
Cuts at: 0 698 939
Size: 698 241
SapI GCTCTTCn'nnn_
Cuts at: 0 619 939
Size: 619 320
SmlI C'TyrA_G
Cuts at: 0 553 939
Size: 553 386
StyI C'CwwG_G
Cuts at: 0 61 317 800 807 939
Size: 61 256 483 7 132
Fragments arranged by size:
483 256 132 61 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 861 939
Size: 861 78
TatI w'GTAC_w
Cuts at: 0 226 757 939
Size: 226 531 182
Fragments arranged by size:
531 226 182
XmnI GAAnn'nnTTC
Cuts at: 0 335 939
Size: 335 604
Enzymes that do cut:
AccI AcuI AflIII AloI ApoI BanII BbsI BciVI
BglI BpmI BpuEI BsaXI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrDI BsrGI BstYI BtsI DraI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI FalI Hin4I HincII
MspA1I NdeI NspI PciI PfoI PvuII SacI SalI
SapI SmlI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DrdI EagI EciI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SanDI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI