(Linear) (Six-base) MAPSORT of: KIEE5442.seq  Check: 404  from: 1  to: 942



KIEE5442.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     769     942 
   Size:        769     173
 
AccI GT'mk_AC

Cuts at:      0     419     942 
   Size:        419     523
 
AflIII A'CryG_T

Cuts at:      0     718     942 
   Size:        718     224
 
AgeI A'CCGG_T

Cuts at:      0     362     942 
   Size:        362     580
 
AleI CACnn'nnGTG

Cuts at:      0     838     942 
   Size:        838     104
 
AvaI C'yCGr_G

Cuts at:      0     127     942 
   Size:        127     815
 
BclI T'GATC_A

Cuts at:      0     401     942 
   Size:        401     541
 
BfrBI ATG'CAT

Cuts at:      0     335     684     942 
   Size:        335     349     258

  Fragments arranged by size: 

                 349     335     258
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     127     942 
   Size:        127     815
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     942 
   Size:         82     860
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     774     942 
   Size:        774     168
 
BsaAI yAC'GTr

Cuts at:      0     719     942 
   Size:        719     223
 
BsaWI w'CCGG_w

Cuts at:      0     362     942 
   Size:        362     580
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     636     942 
   Size:        636     306
 
BseYI C'CCAG_C

Cuts at:      0     158     942 
   Size:        158     784
 
BsmI GAATG_Cn'

Cuts at:      0     337     684     942 
   Size:        337     347     258

  Fragments arranged by size: 

                 347     337     258
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     769     942 
   Size:        769     173
 
BsrFI r'CCGG_y

Cuts at:      0     362     942 
   Size:        362     580
 
Bsu36I CC'TnA_GG

Cuts at:      0     874     942 
   Size:        874      68
 
ClaI AT'CG_AT

Cuts at:      0     152     942 
   Size:        152     790
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     514     942 
   Size:        514     428
 
EaeI y'GGCC_r

Cuts at:      0     371     942 
   Size:        371     571
 
EarI CTCTTCn'nnn_

Cuts at:      0     670     936     942 
   Size:        670     266       6

  Fragments arranged by size: 

                 670     266       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     942 
   Size:         82     860
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     805     942 
   Size:        805     137
 
EcoO109I rG'GnC_Cy

Cuts at:      0     926     942 
   Size:        926      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     241     273     942 
   Size:        241      32     669

  Fragments arranged by size: 

                 669     241      32
 
HincII GTy'rAC

Cuts at:      0     474     942 
   Size:        474     468
 
MslI CAynn'nnrTG

Cuts at:      0     172     838     942 
   Size:        172     666     104

  Fragments arranged by size: 

                 666     172     104
 
MspA1I CmG'CkG

Cuts at:      0     387     942 
   Size:        387     555
 
NdeI CA'TA_TG

Cuts at:      0     376     942 
   Size:        376     566
 
NsiI A_TGCA'T

Cuts at:      0     337     686     942 
   Size:        337     349     256

  Fragments arranged by size: 

                 349     337     256
 
PfoI T'CCnGG_A

Cuts at:      0     409     942 
   Size:        409     533
 
PmlI CAC'GTG

Cuts at:      0     719     942 
   Size:        719     223
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     771     803     942 
   Size:        771      32     139

  Fragments arranged by size: 

                 771     139      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     926     942 
   Size:        926      16
 
PstI C_TGCA'G

Cuts at:      0     453     942 
   Size:        453     489
 
ScaI AGT'ACT

Cuts at:      0     116     942 
   Size:        116     826
 
SfcI C'TryA_G

Cuts at:      0      67     295     449     867     942 
   Size:         67     228     154     418      75

  Fragments arranged by size: 

                 418     228     154      75      67
 
SspI AAT'ATT

Cuts at:      0     618     942 
   Size:        618     324
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     186     942 
   Size:        186     756
 
TatI w'GTAC_w

Cuts at:      0     114     302     942 
   Size:        114     188     640

  Fragments arranged by size: 

                 640     188     114
 

 Enzymes that do cut:

     AarI     AccI   AflIII     AgeI     AleI     AvaI     BclI    BfrBI
     BmrI     BpmI     BsaI    BsaAI    BsaWI    BseRI    BseYI     BsmI
    BspMI    BsrFI   Bsu36I     ClaI     DrdI     EaeI     EarI  Eco57MI
    EcoNI EcoO109I    Hin4I   HincII     MslI   MspA1I     NdeI     NsiI
     PfoI     PmlI     PpiI    PpuMI     PstI     ScaI     SfcI     SspI
    TaqII     TatI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AhdI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaXI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     EagI
     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PmeI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI