Restriction map for
pFN21AE5441
(Linear) (Six-base) MAPSORT of: KIEE5441.seq Check: 9960 from: 1 to: 951
KIEE5441.orf
With 182 enzymes: SgfI *
January 29, 2014 12:00 ..
AccI GT'mk_AC
Cuts at: 0 76 821 951
Size: 76 745 130
Fragments arranged by size:
745 130 76
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 951
Size: 613 338
AfeI AGC'GCT
Cuts at: 0 445 951
Size: 445 506
AlwNI CAG_nnn'CTG
Cuts at: 0 138 248 582 591 951
Size: 138 110 334 9 360
Fragments arranged by size:
360 334 138 110 9
ApaI G_GGCC'C
Cuts at: 0 733 909 951
Size: 733 176 42
Fragments arranged by size:
733 176 42
AvaI C'yCGr_G
Cuts at: 0 439 814 951
Size: 439 375 137
Fragments arranged by size:
439 375 137
BanI G'GyrC_C
Cuts at: 0 46 409 754 915 951
Size: 46 363 345 161 36
Fragments arranged by size:
363 345 161 46 36
BanII G_rGCy'C
Cuts at: 0 383 733 909 931 951
Size: 383 350 176 22 20
Fragments arranged by size:
383 350 176 22 20
BbeI G_GCGC'C
Cuts at: 0 758 951
Size: 758 193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 778 812 951
Size: 778 34 139
Fragments arranged by size:
778 139 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 95 951
Size: 95 856
BclI T'GATC_A
Cuts at: 0 254 951
Size: 254 697
BglI GCCn_nnn'nGGC
Cuts at: 0 913 951
Size: 913 38
Bme1580I G_kGCm'C
Cuts at: 0 733 909 918 951
Size: 733 176 9 33
Fragments arranged by size:
733 176 33 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 951
Size: 32 919
Bpu10I CC'TnA_GC
Cuts at: 0 893 951
Size: 893 58
BsaHI Gr'CG_yC
Cuts at: 0 73 755 951
Size: 73 682 196
Fragments arranged by size:
682 196 73
BseYI C'CCAG_C
Cuts at: 0 21 114 131 248 315 330 536 782
Size: 21 93 17 117 67 15 206 246
Cuts at: 782 951
Size: 169
Fragments arranged by size:
246 206 169 117 93 67 21 17
15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 895 951
Size: 437 458 56
Fragments arranged by size:
458 437 56
BsmBI CGTCTCn'nnnn_
Cuts at: 0 442 951
Size: 442 509
Bsp1286I G_dGCh'C
Cuts at: 0 383 733 909 918 931 951
Size: 383 350 176 9 13 20
Fragments arranged by size:
383 350 176 20 13 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 217 951
Size: 217 734
BsrBI CCG'CTC
Cuts at: 0 465 702 845 951
Size: 465 237 143 106
Fragments arranged by size:
465 237 143 106
BsrDI GCAATG_nn'
Cuts at: 0 422 951
Size: 422 529
BsrFI r'CCGG_y
Cuts at: 0 682 769 951
Size: 682 87 182
Fragments arranged by size:
682 182 87
BssHII G'CGCG_C
Cuts at: 0 888 943 951
Size: 888 55 8
Fragments arranged by size:
888 55 8
BstAPI GCAn_nnn'nTGC
Cuts at: 0 424 873 951
Size: 424 449 78
Fragments arranged by size:
449 424 78
BstXI CCAn_nnnn'nTGG
Cuts at: 0 208 931 951
Size: 208 723 20
Fragments arranged by size:
723 208 20
BstYI r'GATC_y
Cuts at: 0 527 951
Size: 527 424
Bsu36I CC'TnA_GG
Cuts at: 0 910 951
Size: 910 41
BtgI C'CryG_G
Cuts at: 0 882 951
Size: 882 69
BtsI GCAGTG_nn'
Cuts at: 0 219 257 519 951
Size: 219 38 262 432
Fragments arranged by size:
432 262 219 38
DraIII CAC_nnn'GTG
Cuts at: 0 390 951
Size: 390 561
DrdI GACnn_nn'nnGTC
Cuts at: 0 616 951
Size: 616 335
EaeI y'GGCC_r
Cuts at: 0 95 767 951
Size: 95 672 184
Fragments arranged by size:
672 184 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 613 951
Size: 32 581 338
Fragments arranged by size:
581 338 32
EcoO109I rG'GnC_Cy
Cuts at: 0 212 320 468 655 729 730 905 906
Size: 212 108 148 187 74 1 175 1
Cuts at: 906 951
Size: 45
Fragments arranged by size:
212 187 175 148 108 74 45 1
1
FspI TGC'GCA
Cuts at: 0 225 839 951
Size: 225 614 112
Fragments arranged by size:
614 225 112
HaeII r_GCGC'y
Cuts at: 0 19 447 758 951
Size: 19 428 311 193
Fragments arranged by size:
428 311 193 19
HincII GTy'rAC
Cuts at: 0 77 951
Size: 77 874
KasI G'GCGC_C
Cuts at: 0 754 951
Size: 754 197
MspA1I CmG'CkG
Cuts at: 0 138 248 330 782 884 951
Size: 138 110 82 452 102 67
Fragments arranged by size:
452 138 110 102 82 67
NaeI GCC'GGC
Cuts at: 0 684 771 951
Size: 684 87 180
Fragments arranged by size:
684 180 87
NarI GG'CG_CC
Cuts at: 0 755 951
Size: 755 196
NgoMIV G'CCGG_C
Cuts at: 0 682 769 951
Size: 682 87 182
Fragments arranged by size:
682 182 87
NspI r_CATG'y
Cuts at: 0 675 951
Size: 675 276
PasI CC'CwG_GG
Cuts at: 0 27 951
Size: 27 924
PflMI CCAn_nnn'nTGG
Cuts at: 0 121 248 360 951
Size: 121 127 112 591
Fragments arranged by size:
591 127 121 112
PfoI T'CCnGG_A
Cuts at: 0 397 951
Size: 397 554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 328 360 951
Size: 328 32 591
Fragments arranged by size:
591 328 32
PpuMI rG'GwC_Cy
Cuts at: 0 212 468 655 951
Size: 212 256 187 296
Fragments arranged by size:
296 256 212 187
PspOMI G'GGCC_C
Cuts at: 0 729 905 951
Size: 729 176 46
Fragments arranged by size:
729 176 46
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 280 312 951
Size: 280 32 639
Fragments arranged by size:
639 280 32
PstI C_TGCA'G
Cuts at: 0 587 625 951
Size: 587 38 326
Fragments arranged by size:
587 326 38
PvuII CAG'CTG
Cuts at: 0 138 330 782 951
Size: 138 192 452 169
Fragments arranged by size:
452 192 169 138
SacII CC_GC'GG
Cuts at: 0 885 951
Size: 885 66
SalI G'TCGA_C
Cuts at: 0 75 951
Size: 75 876
SanDI GG'GwC_CC
Cuts at: 0 212 655 951
Size: 212 443 296
Fragments arranged by size:
443 296 212
SfcI C'TryA_G
Cuts at: 0 583 621 951
Size: 583 38 330
Fragments arranged by size:
583 330 38
SfoI GGC'GCC
Cuts at: 0 756 951
Size: 756 195
SmaI CCC'GGG
Cuts at: 0 816 951
Size: 816 135
SmlI C'TyrA_G
Cuts at: 0 439 951
Size: 439 512
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 240 338 352 363 532 840 951
Size: 19 221 98 14 11 169 308 111
Fragments arranged by size:
308 221 169 111 98 19 14 11
TatI w'GTAC_w
Cuts at: 0 632 951
Size: 632 319
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 393 425 661 693 951
Size: 393 32 236 32 258
Fragments arranged by size:
393 258 236 32 32
XhoI C'TCGA_G
Cuts at: 0 439 951
Size: 439 512
XmaI C'CCGG_G
Cuts at: 0 814 951
Size: 814 137
Enzymes that do cut:
AccI AcuI AfeI AlwNI ApaI AvaI BanI BanII
BbeI BcgI BciVI BclI BglI Bme1580I BpmI Bpu10I
BsaHI BseYI BsgI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraIII DrdI EaeI Eco57MI EcoO109I FspI HaeII HincII
KasI MspA1I NaeI NarI NgoMIV NspI PasI PflMI
PfoI PpiI PpuMI PspOMI PsrI PstI PvuII SacII
SalI SanDI SfcI SfoI SmaI SmlI TaqII TatI
TstI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI BspEI
BspHI BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspAI Hin4I HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PshAI
PsiI PspXI PvuI RsrII SacI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI Tth111I XbaI XcmI XmnI
ZraI